Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coatin...
Published in: | Colloids and Surfaces B: Biointerfaces |
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/69f69d65-b862-4423-b3d7-98973889a544 2023-05-15T14:01:24+02:00 Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy Jayawardena, Menuk B. Yee, Lachlan H. Rainbow, Ian J. Bergquist, Peter Such, Christopher Steinberg, Peter D. Kjelleberg, Staffan J. 2011-02-01 https://researchers.mq.edu.au/en/publications/69f69d65-b862-4423-b3d7-98973889a544 https://doi.org/10.1016/j.colsurfb.2010.08.042 eng eng info:eu-repo/semantics/closedAccess Jayawardena , M B , Yee , L H , Rainbow , I J , Bergquist , P , Such , C , Steinberg , P D & Kjelleberg , S J 2011 , ' Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy ' , Colloids and Surfaces B: Biointerfaces , vol. 82 , no. 2 , pp. 291-296 . https://doi.org/10.1016/j.colsurfb.2010.08.042 Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA article 2011 ftmacquarieunicr https://doi.org/10.1016/j.colsurfb.2010.08.042 2022-11-06T06:47:36Z Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Antarc* Antarctica Macquarie University Research Portal Colloids and Surfaces B: Biointerfaces 82 2 291 296 |
institution |
Open Polar |
collection |
Macquarie University Research Portal |
op_collection_id |
ftmacquarieunicr |
language |
English |
topic |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA |
spellingShingle |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA Jayawardena, Menuk B. Yee, Lachlan H. Rainbow, Ian J. Bergquist, Peter Such, Christopher Steinberg, Peter D. Kjelleberg, Staffan J. Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
topic_facet |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA |
description |
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple (TM), was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIP(HLL)) and Candida antarctica lipase A (LIP(CA)) within a transparent latex coating. Addition of Teric Surfactants (C(16) non-ionic Teric 475, 1.8% (w/w) or C(10) non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIP(HLL) and LIP(CA) to the surface of a latex coating. An alpha-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 mu m of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. (C) 2010 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Jayawardena, Menuk B. Yee, Lachlan H. Rainbow, Ian J. Bergquist, Peter Such, Christopher Steinberg, Peter D. Kjelleberg, Staffan J. |
author_facet |
Jayawardena, Menuk B. Yee, Lachlan H. Rainbow, Ian J. Bergquist, Peter Such, Christopher Steinberg, Peter D. Kjelleberg, Staffan J. |
author_sort |
Jayawardena, Menuk B. |
title |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_short |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_fullStr |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full_unstemmed |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_sort |
surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
publishDate |
2011 |
url |
https://researchers.mq.edu.au/en/publications/69f69d65-b862-4423-b3d7-98973889a544 https://doi.org/10.1016/j.colsurfb.2010.08.042 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Jayawardena , M B , Yee , L H , Rainbow , I J , Bergquist , P , Such , C , Steinberg , P D & Kjelleberg , S J 2011 , ' Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy ' , Colloids and Surfaces B: Biointerfaces , vol. 82 , no. 2 , pp. 291-296 . https://doi.org/10.1016/j.colsurfb.2010.08.042 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.colsurfb.2010.08.042 |
container_title |
Colloids and Surfaces B: Biointerfaces |
container_volume |
82 |
container_issue |
2 |
container_start_page |
291 |
op_container_end_page |
296 |
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