Double stranded RNA is processed differently in two oyster species
Ostreid herpes virus causes serious disease in the Pacific oyster ( Crassostrea gigas ), but not in the Sydney Rock Oyster ( Saccostrea glomerata ). To investigate differences in disease progression, we injected oysters with double stranded RNA (dsRNA). dsRNA is known to mimic viral infection, and c...
Published in: | Developmental & Comparative Immunology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2017
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Online Access: | https://researchers.mq.edu.au/en/publications/35727286-cf49-4d4a-b0b1-0f44eea79284 https://doi.org/10.1016/j.dci.2017.06.017 http://www.scopus.com/inward/record.url?scp=85021803817&partnerID=8YFLogxK |
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/35727286-cf49-4d4a-b0b1-0f44eea79284 2023-05-15T15:57:43+02:00 Double stranded RNA is processed differently in two oyster species Masood, Muhammad Herberstein, Marie E. Raftos, David A. Nair, Sham V. 2017-11-01 https://researchers.mq.edu.au/en/publications/35727286-cf49-4d4a-b0b1-0f44eea79284 https://doi.org/10.1016/j.dci.2017.06.017 http://www.scopus.com/inward/record.url?scp=85021803817&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Masood , M , Herberstein , M E , Raftos , D A & Nair , S V 2017 , ' Double stranded RNA is processed differently in two oyster species ' , Developmental and Comparative Immunology , vol. 76 , pp. 285-291 . https://doi.org/10.1016/j.dci.2017.06.017 invertebrate immune response virus oyster poly (I:C) article 2017 ftmacquarieunicr https://doi.org/10.1016/j.dci.2017.06.017 2022-11-06T06:56:11Z Ostreid herpes virus causes serious disease in the Pacific oyster ( Crassostrea gigas ), but not in the Sydney Rock Oyster ( Saccostrea glomerata ). To investigate differences in disease progression, we injected oysters with double stranded RNA (dsRNA). dsRNA is known to mimic viral infection, and can evoke immune responses when Toll-like receptors detect the dsRNA, leading to the production of type 1 interferon and inflammation cytokines. The uptake and processing of dsRNA was tracked in gill and mantle tissue of Crassostrea gigas and Saccostrea glomerata after injection of fluorochrome labelled poly (I:C) dsRNA. The two species showed significant differences in tissue uptake and clearance, and differences in immune responses confirmed by real time PCR. These results showed that S. glomerata was more efficient in processing dsRNA than C. gigas , and that the gill tissue is an important site of dsRNA processing and response. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Macquarie University Research Portal Pacific Developmental & Comparative Immunology 76 285 291 |
institution |
Open Polar |
collection |
Macquarie University Research Portal |
op_collection_id |
ftmacquarieunicr |
language |
English |
topic |
invertebrate immune response virus oyster poly (I:C) |
spellingShingle |
invertebrate immune response virus oyster poly (I:C) Masood, Muhammad Herberstein, Marie E. Raftos, David A. Nair, Sham V. Double stranded RNA is processed differently in two oyster species |
topic_facet |
invertebrate immune response virus oyster poly (I:C) |
description |
Ostreid herpes virus causes serious disease in the Pacific oyster ( Crassostrea gigas ), but not in the Sydney Rock Oyster ( Saccostrea glomerata ). To investigate differences in disease progression, we injected oysters with double stranded RNA (dsRNA). dsRNA is known to mimic viral infection, and can evoke immune responses when Toll-like receptors detect the dsRNA, leading to the production of type 1 interferon and inflammation cytokines. The uptake and processing of dsRNA was tracked in gill and mantle tissue of Crassostrea gigas and Saccostrea glomerata after injection of fluorochrome labelled poly (I:C) dsRNA. The two species showed significant differences in tissue uptake and clearance, and differences in immune responses confirmed by real time PCR. These results showed that S. glomerata was more efficient in processing dsRNA than C. gigas , and that the gill tissue is an important site of dsRNA processing and response. |
format |
Article in Journal/Newspaper |
author |
Masood, Muhammad Herberstein, Marie E. Raftos, David A. Nair, Sham V. |
author_facet |
Masood, Muhammad Herberstein, Marie E. Raftos, David A. Nair, Sham V. |
author_sort |
Masood, Muhammad |
title |
Double stranded RNA is processed differently in two oyster species |
title_short |
Double stranded RNA is processed differently in two oyster species |
title_full |
Double stranded RNA is processed differently in two oyster species |
title_fullStr |
Double stranded RNA is processed differently in two oyster species |
title_full_unstemmed |
Double stranded RNA is processed differently in two oyster species |
title_sort |
double stranded rna is processed differently in two oyster species |
publishDate |
2017 |
url |
https://researchers.mq.edu.au/en/publications/35727286-cf49-4d4a-b0b1-0f44eea79284 https://doi.org/10.1016/j.dci.2017.06.017 http://www.scopus.com/inward/record.url?scp=85021803817&partnerID=8YFLogxK |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Masood , M , Herberstein , M E , Raftos , D A & Nair , S V 2017 , ' Double stranded RNA is processed differently in two oyster species ' , Developmental and Comparative Immunology , vol. 76 , pp. 285-291 . https://doi.org/10.1016/j.dci.2017.06.017 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.dci.2017.06.017 |
container_title |
Developmental & Comparative Immunology |
container_volume |
76 |
container_start_page |
285 |
op_container_end_page |
291 |
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1766393409982955520 |