The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus
We successfully isolated a lipase gene (designated lipPA) directly from the genomic DNA of an Antarctic isolate of Penicillium allii using PCR and a suite of degenerate primers specifically designed to target two conserved regions of fungal lipase genes. We applied the biolistic transformation syste...
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2003
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/0f90bbbd-5aa5-4a78-aa64-0c267ea66d45 2023-05-15T13:58:30+02:00 The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus Bradner, J. Ron Bell, Philip J L Te'o, V. S Junior Nevalainen, K. M Helena 2003-12 https://researchers.mq.edu.au/en/publications/0f90bbbd-5aa5-4a78-aa64-0c267ea66d45 https://doi.org/10.1007/s00294-003-0440-1 http://www.scopus.com/inward/record.url?scp=0347513481&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Bradner , J R , Bell , P J L , Te'o , V S J & Nevalainen , K M H 2003 , ' The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus ' , Current Genetics , vol. 44 , no. 4 , pp. 224-230 . https://doi.org/10.1007/s00294-003-0440-1 article 2003 ftmacquarieunicr https://doi.org/10.1007/s00294-003-0440-1 2022-11-06T06:32:13Z We successfully isolated a lipase gene (designated lipPA) directly from the genomic DNA of an Antarctic isolate of Penicillium allii using PCR and a suite of degenerate primers specifically designed to target two conserved regions of fungal lipase genes. We applied the biolistic transformation system to successfully integrate the lipPA gene into a heterologous fungal host, Trichoderma reesei, one of the most powerful secretors of extracellular proteins, and induced the transformant to secrete an active lipase into the growth medium. The recombinant lipase had a temperature optimum of 25 °C at pH 7.9 and retained greater than 50% of the maximum activity from 10 °C to 35 °C and over a pH range from 4.0 to 8.5. Article in Journal/Newspaper Antarc* Antarctic Macquarie University Research Portal Antarctic Current Genetics 44 4 224 230 |
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Open Polar |
collection |
Macquarie University Research Portal |
op_collection_id |
ftmacquarieunicr |
language |
English |
description |
We successfully isolated a lipase gene (designated lipPA) directly from the genomic DNA of an Antarctic isolate of Penicillium allii using PCR and a suite of degenerate primers specifically designed to target two conserved regions of fungal lipase genes. We applied the biolistic transformation system to successfully integrate the lipPA gene into a heterologous fungal host, Trichoderma reesei, one of the most powerful secretors of extracellular proteins, and induced the transformant to secrete an active lipase into the growth medium. The recombinant lipase had a temperature optimum of 25 °C at pH 7.9 and retained greater than 50% of the maximum activity from 10 °C to 35 °C and over a pH range from 4.0 to 8.5. |
format |
Article in Journal/Newspaper |
author |
Bradner, J. Ron Bell, Philip J L Te'o, V. S Junior Nevalainen, K. M Helena |
spellingShingle |
Bradner, J. Ron Bell, Philip J L Te'o, V. S Junior Nevalainen, K. M Helena The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
author_facet |
Bradner, J. Ron Bell, Philip J L Te'o, V. S Junior Nevalainen, K. M Helena |
author_sort |
Bradner, J. Ron |
title |
The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
title_short |
The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
title_full |
The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
title_fullStr |
The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
title_full_unstemmed |
The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus |
title_sort |
application of pcr for the isolation of a lipase gene from the genomic dna of an antarctic microfungus |
publishDate |
2003 |
url |
https://researchers.mq.edu.au/en/publications/0f90bbbd-5aa5-4a78-aa64-0c267ea66d45 https://doi.org/10.1007/s00294-003-0440-1 http://www.scopus.com/inward/record.url?scp=0347513481&partnerID=8YFLogxK |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Bradner , J R , Bell , P J L , Te'o , V S J & Nevalainen , K M H 2003 , ' The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus ' , Current Genetics , vol. 44 , no. 4 , pp. 224-230 . https://doi.org/10.1007/s00294-003-0440-1 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1007/s00294-003-0440-1 |
container_title |
Current Genetics |
container_volume |
44 |
container_issue |
4 |
container_start_page |
224 |
op_container_end_page |
230 |
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1766266825542205440 |