Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses
Viruses belonging to the family Malacoherpesviridae currently pose a serious threat to global production of the Pacific oyster, Crassostrea gigas. Hemolymph extracts from C. gigas are known to have potent antiviral activity. The compound(s) responsible for this broad-spectrum antiviral activity in o...
Published in: | Antiviral Research |
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2014
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Online Access: | https://researchers.mq.edu.au/en/publications/061efc26-e1af-4fce-985b-47364c4fbe70 https://doi.org/10.1016/j.antiviral.2014.08.010 http://www.scopus.com/inward/record.url?scp=84907808176&partnerID=8YFLogxK |
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/061efc26-e1af-4fce-985b-47364c4fbe70 2024-06-02T08:05:36+00:00 Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses Green, Timothy J. Robinson, Nick Chataway, Tim Benkendorff, Kirsten O'Connor, Wayne Speck, Peter 2014-10 https://researchers.mq.edu.au/en/publications/061efc26-e1af-4fce-985b-47364c4fbe70 https://doi.org/10.1016/j.antiviral.2014.08.010 http://www.scopus.com/inward/record.url?scp=84907808176&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Green , T J , Robinson , N , Chataway , T , Benkendorff , K , O'Connor , W & Speck , P 2014 , ' Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses ' , Antiviral Research , vol. 110 , pp. 168-174 . https://doi.org/10.1016/j.antiviral.2014.08.010 Antiviral Cavortin Crassostrea Extracellular superoxide dismutase Herpesvirus article 2014 ftmacquarieunicr https://doi.org/10.1016/j.antiviral.2014.08.010 2024-05-07T03:23:29Z Viruses belonging to the family Malacoherpesviridae currently pose a serious threat to global production of the Pacific oyster, Crassostrea gigas. Hemolymph extracts from C. gigas are known to have potent antiviral activity. The compound(s) responsible for this broad-spectrum antiviral activity in oyster hemolymph have not been identified. The objective of this study was to identify these antiviral compound(s) and establish whether hemolymph antiviral activity is under genetic control in the Australian C. gigas population. Hemolymph antiviral activity of 18 family lines of C. gigas were assayed using a herpes simplex virus type 1 (HSV-1) and Vero cell plaque reduction assay. Differences in anti-HSV-1 activity between the family lines were observed (p < 0.001) with heritability estimated to be low (h 2 = 0.21). A glycoprotein that inhibits HSV-1 replication was identified by resolving oyster hemolymph by native-polyacrylamide gel electrophoresis (PAGE) and assaying extracted protein fractions using the HSV-1 and Vero cell plaque assay. Highest anti-HSV-1 activity corresponded with an N-linked glycoprotein with an estimated molecular mass of 21 kDa under non-reducing SDS-PAGE conditions. Amino acid sequencing by tandem mass spectrometry revealed this protein matched the major hemolymph protein, termed cavortin. Our results provide further evidence that cavortin is a multifunctional protein involved in immunity and that assays associated with its activity might be useful for marker-assisted selection of disease resistant oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Macquarie University Research Portal Pacific Antiviral Research 110 168 174 |
institution |
Open Polar |
collection |
Macquarie University Research Portal |
op_collection_id |
ftmacquarieunicr |
language |
English |
topic |
Antiviral Cavortin Crassostrea Extracellular superoxide dismutase Herpesvirus |
spellingShingle |
Antiviral Cavortin Crassostrea Extracellular superoxide dismutase Herpesvirus Green, Timothy J. Robinson, Nick Chataway, Tim Benkendorff, Kirsten O'Connor, Wayne Speck, Peter Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
topic_facet |
Antiviral Cavortin Crassostrea Extracellular superoxide dismutase Herpesvirus |
description |
Viruses belonging to the family Malacoherpesviridae currently pose a serious threat to global production of the Pacific oyster, Crassostrea gigas. Hemolymph extracts from C. gigas are known to have potent antiviral activity. The compound(s) responsible for this broad-spectrum antiviral activity in oyster hemolymph have not been identified. The objective of this study was to identify these antiviral compound(s) and establish whether hemolymph antiviral activity is under genetic control in the Australian C. gigas population. Hemolymph antiviral activity of 18 family lines of C. gigas were assayed using a herpes simplex virus type 1 (HSV-1) and Vero cell plaque reduction assay. Differences in anti-HSV-1 activity between the family lines were observed (p < 0.001) with heritability estimated to be low (h 2 = 0.21). A glycoprotein that inhibits HSV-1 replication was identified by resolving oyster hemolymph by native-polyacrylamide gel electrophoresis (PAGE) and assaying extracted protein fractions using the HSV-1 and Vero cell plaque assay. Highest anti-HSV-1 activity corresponded with an N-linked glycoprotein with an estimated molecular mass of 21 kDa under non-reducing SDS-PAGE conditions. Amino acid sequencing by tandem mass spectrometry revealed this protein matched the major hemolymph protein, termed cavortin. Our results provide further evidence that cavortin is a multifunctional protein involved in immunity and that assays associated with its activity might be useful for marker-assisted selection of disease resistant oysters. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy J. Robinson, Nick Chataway, Tim Benkendorff, Kirsten O'Connor, Wayne Speck, Peter |
author_facet |
Green, Timothy J. Robinson, Nick Chataway, Tim Benkendorff, Kirsten O'Connor, Wayne Speck, Peter |
author_sort |
Green, Timothy J. |
title |
Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
title_short |
Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
title_full |
Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
title_fullStr |
Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
title_full_unstemmed |
Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses |
title_sort |
evidence that the major hemolymph protein of the pacific oyster, crassostrea gigas , has antiviral activity against herpesviruses |
publishDate |
2014 |
url |
https://researchers.mq.edu.au/en/publications/061efc26-e1af-4fce-985b-47364c4fbe70 https://doi.org/10.1016/j.antiviral.2014.08.010 http://www.scopus.com/inward/record.url?scp=84907808176&partnerID=8YFLogxK |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Green , T J , Robinson , N , Chataway , T , Benkendorff , K , O'Connor , W & Speck , P 2014 , ' Evidence that the major hemolymph protein of the Pacific oyster, Crassostrea gigas , has antiviral activity against herpesviruses ' , Antiviral Research , vol. 110 , pp. 168-174 . https://doi.org/10.1016/j.antiviral.2014.08.010 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.antiviral.2014.08.010 |
container_title |
Antiviral Research |
container_volume |
110 |
container_start_page |
168 |
op_container_end_page |
174 |
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1800750455570038784 |