Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2
Previous work suggests that larvae from Sydney rock oysters that have been selectively bred for fast growth and disease resistance are more resilient to the impacts of ocean acidification than nonselected, wild-type oysters. In this study, we used proteomics to investigate the molecular differences...
Published in: | Molecular Ecology |
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Online Access: | https://researchers.mq.edu.au/en/publications/01814ffe-6491-4e00-9c83-7f62f257a9c3 https://doi.org/10.1111/mec.13111 http://www.scopus.com/inward/record.url?scp=84924981536&partnerID=8YFLogxK http://purl.org/au-research/grants/arc/DP120101946 |
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ftmacquarieunicr:oai:https://researchers.mq.edu.au:publications/01814ffe-6491-4e00-9c83-7f62f257a9c3 2024-04-28T08:34:55+00:00 Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 Thompson, E. L. O'Connor, W. Parker, L. Ross, P. Raftos, D. A. 2015-03-01 https://researchers.mq.edu.au/en/publications/01814ffe-6491-4e00-9c83-7f62f257a9c3 https://doi.org/10.1111/mec.13111 http://www.scopus.com/inward/record.url?scp=84924981536&partnerID=8YFLogxK http://purl.org/au-research/grants/arc/DP120101946 eng eng info:eu-repo/semantics/closedAccess Thompson , E L , O'Connor , W , Parker , L , Ross , P & Raftos , D A 2015 , ' Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 ' , Molecular Ecology , vol. 24 , no. 6 , pp. 1248-1262 . https://doi.org/10.1111/mec.13111 carbon dioxide CO2 environmental proteomics Saccostrea glomerata selective breeding Sydney rock oyster article 2015 ftmacquarieunicr https://doi.org/10.1111/mec.13111 2024-04-02T17:10:09Z Previous work suggests that larvae from Sydney rock oysters that have been selectively bred for fast growth and disease resistance are more resilient to the impacts of ocean acidification than nonselected, wild-type oysters. In this study, we used proteomics to investigate the molecular differences between oyster populations in adult Sydney rock oysters and to identify whether these form the basis for observations seen in larvae. Adult oysters from a selective breeding line (B2) and nonselected wild types (WT) were exposed for 4 weeks to elevated pCO 2 (856 μatm) before their proteomes were compared to those of oysters held under ambient conditions (375 μatm pCO 2 ). Exposure to elevated pCO 2 resulted in substantial changes in the proteomes of oysters from both the selectively bred and wild-type populations. When biological functions were assigned, these differential proteins fell into five broad, potentially interrelated categories of subcellular functions, in both oyster populations. These functional categories were energy production, cellular stress responses, the cytoskeleton, protein synthesis and cell signalling. In the wild-type population, proteins were predominantly upregulated. However, unexpectedly, these cellular systems were downregulated in the selectively bred oyster population, indicating cellular dysfunction. We argue that this reflects a trade-off, whereby an adaptive capacity for enhanced mitochondrial energy production in the selectively bred population may help to protect larvae from the effects of elevated CO 2 , whilst being deleterious to adult oysters. Article in Journal/Newspaper Ocean acidification Macquarie University Research Portal Molecular Ecology 24 6 1248 1262 |
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Open Polar |
collection |
Macquarie University Research Portal |
op_collection_id |
ftmacquarieunicr |
language |
English |
topic |
carbon dioxide CO2 environmental proteomics Saccostrea glomerata selective breeding Sydney rock oyster |
spellingShingle |
carbon dioxide CO2 environmental proteomics Saccostrea glomerata selective breeding Sydney rock oyster Thompson, E. L. O'Connor, W. Parker, L. Ross, P. Raftos, D. A. Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
topic_facet |
carbon dioxide CO2 environmental proteomics Saccostrea glomerata selective breeding Sydney rock oyster |
description |
Previous work suggests that larvae from Sydney rock oysters that have been selectively bred for fast growth and disease resistance are more resilient to the impacts of ocean acidification than nonselected, wild-type oysters. In this study, we used proteomics to investigate the molecular differences between oyster populations in adult Sydney rock oysters and to identify whether these form the basis for observations seen in larvae. Adult oysters from a selective breeding line (B2) and nonselected wild types (WT) were exposed for 4 weeks to elevated pCO 2 (856 μatm) before their proteomes were compared to those of oysters held under ambient conditions (375 μatm pCO 2 ). Exposure to elevated pCO 2 resulted in substantial changes in the proteomes of oysters from both the selectively bred and wild-type populations. When biological functions were assigned, these differential proteins fell into five broad, potentially interrelated categories of subcellular functions, in both oyster populations. These functional categories were energy production, cellular stress responses, the cytoskeleton, protein synthesis and cell signalling. In the wild-type population, proteins were predominantly upregulated. However, unexpectedly, these cellular systems were downregulated in the selectively bred oyster population, indicating cellular dysfunction. We argue that this reflects a trade-off, whereby an adaptive capacity for enhanced mitochondrial energy production in the selectively bred population may help to protect larvae from the effects of elevated CO 2 , whilst being deleterious to adult oysters. |
format |
Article in Journal/Newspaper |
author |
Thompson, E. L. O'Connor, W. Parker, L. Ross, P. Raftos, D. A. |
author_facet |
Thompson, E. L. O'Connor, W. Parker, L. Ross, P. Raftos, D. A. |
author_sort |
Thompson, E. L. |
title |
Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
title_short |
Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
title_full |
Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
title_fullStr |
Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
title_full_unstemmed |
Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 |
title_sort |
differential proteomic responses of selectively bred and wild-type sydney rock oyster populations exposed to elevated co 2 |
publishDate |
2015 |
url |
https://researchers.mq.edu.au/en/publications/01814ffe-6491-4e00-9c83-7f62f257a9c3 https://doi.org/10.1111/mec.13111 http://www.scopus.com/inward/record.url?scp=84924981536&partnerID=8YFLogxK http://purl.org/au-research/grants/arc/DP120101946 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Thompson , E L , O'Connor , W , Parker , L , Ross , P & Raftos , D A 2015 , ' Differential proteomic responses of selectively bred and wild-type Sydney rock oyster populations exposed to elevated CO 2 ' , Molecular Ecology , vol. 24 , no. 6 , pp. 1248-1262 . https://doi.org/10.1111/mec.13111 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/mec.13111 |
container_title |
Molecular Ecology |
container_volume |
24 |
container_issue |
6 |
container_start_page |
1248 |
op_container_end_page |
1262 |
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1797591434081075200 |