Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum
Southern Ocean temperatures are rising due to climate change. Increasing temperature can alter diatom physiology and survival, subsequently affecting primary productivity and distributions. Diatoms are important primary producers and their composition mediates energy and nutrient transfer to higher...
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ftmacquariefig:oai:figshare.com:article/19435721 2023-05-15T13:41:11+02:00 Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum Peta L. Vine 2019-08-02T00:00:00Z https://doi.org/10.25949/19435721.v1 https://figshare.com/articles/thesis/Effects_of_temperature_on_macromolecular_composition_of_the_Antarctic_diatom_Corethron_pennatum/19435721 unknown doi:10.25949/19435721.v1 https://figshare.com/articles/thesis/Effects_of_temperature_on_macromolecular_composition_of_the_Antarctic_diatom_Corethron_pennatum/19435721 In Copyright Other education not elsewhere classified climate change macromolecular composition Ocean temperature Environmental aspects Pennales Climatic factors food web primary productivity phoyophysiology Attenuated Total Reflectance-Fourier Transform Infrares Spectroscopy (ATR-FTIR) Text Thesis 2019 ftmacquariefig https://doi.org/10.25949/19435721.v1 2022-12-28T08:39:55Z Southern Ocean temperatures are rising due to climate change. Increasing temperature can alter diatom physiology and survival, subsequently affecting primary productivity and distributions. Diatoms are important primary producers and their composition mediates energy and nutrient transfer to higher trophic levels. Diatom physiology and macromolecular composition are useful indicators for demonstrating and modelling microalgal response to climate change. Using pulse amplitude modulated fluorometry and Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) spectroscopy, I characterised the physiological response and macromolecular composition of the Antarctic diatom, Corethron pennatum cultured at 0 °C to 5°C. The ATR-FTIR generated data were used to create spectroscopy-based predictive models. The photosynthetic capacity of C. pennatum decreased as temperatures increased, while all cultures eventually failed at 5 °C. As growth temperature increased, unsaturated fatty acid concentrations generally increased, and protein levels decreased slightly. Lipid levels were lowest at the coldest growth temperatures. These findings, particularly the unusual lipid unsaturation at the highest temperatures, show that C. pennatum physiology may differ from many diatoms. Additionally, the model demonstrated a high predictive power (R2 = 0.98), showing that macromolecular composition of C. pennatum is a useful intracellular marker that could be used to model microalgal response to climate change. Thesis Antarc* Antarctic Southern Ocean Research from Macquarie University Antarctic Southern Ocean The Antarctic |
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Research from Macquarie University |
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ftmacquariefig |
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topic |
Other education not elsewhere classified climate change macromolecular composition Ocean temperature Environmental aspects Pennales Climatic factors food web primary productivity phoyophysiology Attenuated Total Reflectance-Fourier Transform Infrares Spectroscopy (ATR-FTIR) |
spellingShingle |
Other education not elsewhere classified climate change macromolecular composition Ocean temperature Environmental aspects Pennales Climatic factors food web primary productivity phoyophysiology Attenuated Total Reflectance-Fourier Transform Infrares Spectroscopy (ATR-FTIR) Peta L. Vine Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
topic_facet |
Other education not elsewhere classified climate change macromolecular composition Ocean temperature Environmental aspects Pennales Climatic factors food web primary productivity phoyophysiology Attenuated Total Reflectance-Fourier Transform Infrares Spectroscopy (ATR-FTIR) |
description |
Southern Ocean temperatures are rising due to climate change. Increasing temperature can alter diatom physiology and survival, subsequently affecting primary productivity and distributions. Diatoms are important primary producers and their composition mediates energy and nutrient transfer to higher trophic levels. Diatom physiology and macromolecular composition are useful indicators for demonstrating and modelling microalgal response to climate change. Using pulse amplitude modulated fluorometry and Attenuated Total Reflectance - Fourier Transform Infrared (ATR-FTIR) spectroscopy, I characterised the physiological response and macromolecular composition of the Antarctic diatom, Corethron pennatum cultured at 0 °C to 5°C. The ATR-FTIR generated data were used to create spectroscopy-based predictive models. The photosynthetic capacity of C. pennatum decreased as temperatures increased, while all cultures eventually failed at 5 °C. As growth temperature increased, unsaturated fatty acid concentrations generally increased, and protein levels decreased slightly. Lipid levels were lowest at the coldest growth temperatures. These findings, particularly the unusual lipid unsaturation at the highest temperatures, show that C. pennatum physiology may differ from many diatoms. Additionally, the model demonstrated a high predictive power (R2 = 0.98), showing that macromolecular composition of C. pennatum is a useful intracellular marker that could be used to model microalgal response to climate change. |
format |
Thesis |
author |
Peta L. Vine |
author_facet |
Peta L. Vine |
author_sort |
Peta L. Vine |
title |
Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
title_short |
Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
title_full |
Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
title_fullStr |
Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
title_full_unstemmed |
Effects of temperature on macromolecular composition of the Antarctic diatom, Corethron pennatum |
title_sort |
effects of temperature on macromolecular composition of the antarctic diatom, corethron pennatum |
publishDate |
2019 |
url |
https://doi.org/10.25949/19435721.v1 https://figshare.com/articles/thesis/Effects_of_temperature_on_macromolecular_composition_of_the_Antarctic_diatom_Corethron_pennatum/19435721 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
doi:10.25949/19435721.v1 https://figshare.com/articles/thesis/Effects_of_temperature_on_macromolecular_composition_of_the_Antarctic_diatom_Corethron_pennatum/19435721 |
op_rights |
In Copyright |
op_doi |
https://doi.org/10.25949/19435721.v1 |
_version_ |
1766146547155730432 |