Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments
Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area...
Published in: | European Journal of Wildlife Research |
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ftluke:oai:jukuri.luke.fi:10024/554952 2024-06-09T07:47:25+00:00 Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund 4100110810 Luonnonvarakeskus true https://jukuri.luke.fi/handle/10024/554952 https://doi.org/10.1007/s10344-024-01808-0 en eng Springer Science and Business Media LLC European Journal of Wildlife Research 10.1007/s10344-024-01808-0 1612-4642 1439-0574 3 70 53 Cite this article: Kopatz, A., Norman, A.J., Spong, G. et al. Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments. Eur J Wildl Res 70, 53 (2024). https://doi.org/10.1007/s10344-024-01808-0 https://jukuri.luke.fi/handle/10024/554952 URN:NBN:fi-fe2024051530864 http://dx.doi.org/10.1007/s10344-024-01808-0 CC BY 4.0 noninvasive sampling single-nucleotide sampling DNA-based transborder monitoring genetic methods microsatellites publication fi=A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä|sv=A1 Originalartikel i en vetenskaplig tidskrift|en=A1 Journal article (refereed), original research| fi=Publisher's version|sv=Publisher's version|en=Publisher's version| ftluke https://doi.org/10.1007/s10344-024-01808-0 2024-05-15T23:38:57Z Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area before being deployed. Around 4,500 brown bears (Ursus arctos) live in Norway, Sweden, and Finland and they are divided into the western (Scandinavian) and eastern (Karelian) population. Both populations have recovered and are connected via asymmetric migration. DNA-based population monitoring in Norway and Sweden uses the same set of genetic markers. With Finland aiming to implement monitoring, we tested the available SNP-panel developed to assess brown bears in Norway and Sweden, on tissue samples from a representative set of 93 legally harvested individuals from Finland. The aim was to test for ascertainment bias and evaluate its suitability for DNA-based transnational-monitoring covering all three countries. We compared results to the performance of microsatellite genotypes of the same individuals in Finland and against SNP-genotypes from individuals sampled in Sweden (N = 95) and Norway (N = 27). In Finland, a higher resolution for individual identification was obtained for SNPs (PI = 1.18E-27) compared to microsatellites (PI = 4.2E-11). Compared to Norway and Sweden, probability of identity of the SNP-panel was slightly higher and expected heterozygosity lower in Finland indicating ascertainment bias. Yet, our evaluation show that the available SNP-panel outperforms the microsatellite panel currently applied in Norway and Sweden. The SNP-panel represents a powerful tool that could aid improving transnational DNA-based monitoring of brown bears across these three countries. Article in Journal/Newspaper karelia* karelian Ursus arctos Natural Resources Institute Finland: Jukuri Norway European Journal of Wildlife Research 70 3 |
institution |
Open Polar |
collection |
Natural Resources Institute Finland: Jukuri |
op_collection_id |
ftluke |
language |
English |
topic |
noninvasive sampling single-nucleotide sampling DNA-based transborder monitoring genetic methods microsatellites |
spellingShingle |
noninvasive sampling single-nucleotide sampling DNA-based transborder monitoring genetic methods microsatellites Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
topic_facet |
noninvasive sampling single-nucleotide sampling DNA-based transborder monitoring genetic methods microsatellites |
description |
Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area before being deployed. Around 4,500 brown bears (Ursus arctos) live in Norway, Sweden, and Finland and they are divided into the western (Scandinavian) and eastern (Karelian) population. Both populations have recovered and are connected via asymmetric migration. DNA-based population monitoring in Norway and Sweden uses the same set of genetic markers. With Finland aiming to implement monitoring, we tested the available SNP-panel developed to assess brown bears in Norway and Sweden, on tissue samples from a representative set of 93 legally harvested individuals from Finland. The aim was to test for ascertainment bias and evaluate its suitability for DNA-based transnational-monitoring covering all three countries. We compared results to the performance of microsatellite genotypes of the same individuals in Finland and against SNP-genotypes from individuals sampled in Sweden (N = 95) and Norway (N = 27). In Finland, a higher resolution for individual identification was obtained for SNPs (PI = 1.18E-27) compared to microsatellites (PI = 4.2E-11). Compared to Norway and Sweden, probability of identity of the SNP-panel was slightly higher and expected heterozygosity lower in Finland indicating ascertainment bias. Yet, our evaluation show that the available SNP-panel outperforms the microsatellite panel currently applied in Norway and Sweden. The SNP-panel represents a powerful tool that could aid improving transnational DNA-based monitoring of brown bears across these three countries. |
author2 |
4100110810 Luonnonvarakeskus |
format |
Article in Journal/Newspaper |
author |
Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund |
author_facet |
Kopatz, Alexander Norman, Anita J. Spong, Göran Valtonen, Mia Kojola, Ilpo Aspi, Jouni Kindberg, Jonas Flagstad, Øystein Kleven, Oddmund |
author_sort |
Kopatz, Alexander |
title |
Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
title_short |
Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
title_full |
Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
title_fullStr |
Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
title_full_unstemmed |
Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments |
title_sort |
expanding the spatial scale in dna-based monitoring schemes: ascertainment bias in transnational assessments |
publisher |
Springer Science and Business Media LLC |
url |
https://jukuri.luke.fi/handle/10024/554952 https://doi.org/10.1007/s10344-024-01808-0 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
karelia* karelian Ursus arctos |
genre_facet |
karelia* karelian Ursus arctos |
op_relation |
European Journal of Wildlife Research 10.1007/s10344-024-01808-0 1612-4642 1439-0574 3 70 53 Cite this article: Kopatz, A., Norman, A.J., Spong, G. et al. Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments. Eur J Wildl Res 70, 53 (2024). https://doi.org/10.1007/s10344-024-01808-0 https://jukuri.luke.fi/handle/10024/554952 URN:NBN:fi-fe2024051530864 http://dx.doi.org/10.1007/s10344-024-01808-0 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.1007/s10344-024-01808-0 |
container_title |
European Journal of Wildlife Research |
container_volume |
70 |
container_issue |
3 |
_version_ |
1801378493155508224 |