The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor.
Infections by the bacterium Aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. In this study, we showed that AsaP1, a toxic 19-kDa metallopeptidase produced by A. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (Aeromona...
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American Society for Microbiology
2009
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ftlshtm:oai:researchonline.lshtm.ac.uk:4003 2024-09-15T17:52:25+00:00 The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. Arnadottir, Helga Hvanndal, Iris Andresdottir, Valgerdur Burr, Sarah E Frey, Joachim Gudmundsdottir, Bjarnheidur K 2009 https://researchonline.lshtm.ac.uk/id/eprint/4003/ unknown American Society for Microbiology Arnadottir, Helga; Hvanndal, Iris; Andresdottir, Valgerdur; Burr, Sarah E <https://researchonline.lshtm.ac.uk/view/creators/icrusbur.html>; Frey, Joachim; Gudmundsdottir, Bjarnheidur K; (2009) The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. Journal of bacteriology, 191 (1). pp. 403-410. ISSN 0021-9193 DOI: https://doi.org/10.1128/JB.00847-08 <https://doi.org/10.1128/JB.00847-08> Article PeerReviewed 2009 ftlshtm https://doi.org/10.1128/JB.00847-08 2024-07-19T04:44:16Z Infections by the bacterium Aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. In this study, we showed that AsaP1, a toxic 19-kDa metallopeptidase produced by A. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (Aeromonas type) (MEROPS ID M35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases. The structural gene of AsaP1 was sequenced and found to be highly conserved among gram-negative bacteria. An isogenic Delta asaP1 A. salmonicida subsp. achromogenes strain was constructed, and its ability to infect fish was compared with that of the wild-type (wt) strain. The Delta asaP1 strain was found to infect Arctic charr, Atlantic salmon, and Atlantic cod, but its virulence was decreased relative to that of the wt strain. The 50% lethal dose of the AsaP1 mutant was 10-fold higher in charr and 5-fold higher in salmon than that of the wt strain. The pathology induced by the AsaP1-deficient strain was also different from that of the wt strain. Furthermore, the mutant established significant bacterial colonization in all observed organs without any signs of a host response in the infected tissue. AsaP1 is therefore the first member of the M35 family that has been shown to be a bacterial virulence factor. Article in Journal/Newspaper Arctic charr atlantic cod Atlantic salmon London School of Hygiene & Tropical Medicine: LSHTM Research Online Journal of Bacteriology 191 1 403 410 |
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London School of Hygiene & Tropical Medicine: LSHTM Research Online |
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ftlshtm |
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description |
Infections by the bacterium Aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. In this study, we showed that AsaP1, a toxic 19-kDa metallopeptidase produced by A. salmonicida subsp. achromogenes, belongs to the group of extracellular peptidases (Aeromonas type) (MEROPS ID M35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases. The structural gene of AsaP1 was sequenced and found to be highly conserved among gram-negative bacteria. An isogenic Delta asaP1 A. salmonicida subsp. achromogenes strain was constructed, and its ability to infect fish was compared with that of the wild-type (wt) strain. The Delta asaP1 strain was found to infect Arctic charr, Atlantic salmon, and Atlantic cod, but its virulence was decreased relative to that of the wt strain. The 50% lethal dose of the AsaP1 mutant was 10-fold higher in charr and 5-fold higher in salmon than that of the wt strain. The pathology induced by the AsaP1-deficient strain was also different from that of the wt strain. Furthermore, the mutant established significant bacterial colonization in all observed organs without any signs of a host response in the infected tissue. AsaP1 is therefore the first member of the M35 family that has been shown to be a bacterial virulence factor. |
format |
Article in Journal/Newspaper |
author |
Arnadottir, Helga Hvanndal, Iris Andresdottir, Valgerdur Burr, Sarah E Frey, Joachim Gudmundsdottir, Bjarnheidur K |
spellingShingle |
Arnadottir, Helga Hvanndal, Iris Andresdottir, Valgerdur Burr, Sarah E Frey, Joachim Gudmundsdottir, Bjarnheidur K The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
author_facet |
Arnadottir, Helga Hvanndal, Iris Andresdottir, Valgerdur Burr, Sarah E Frey, Joachim Gudmundsdottir, Bjarnheidur K |
author_sort |
Arnadottir, Helga |
title |
The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
title_short |
The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
title_full |
The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
title_fullStr |
The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
title_full_unstemmed |
The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. |
title_sort |
asap1 peptidase of aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family m35) and a major virulence factor. |
publisher |
American Society for Microbiology |
publishDate |
2009 |
url |
https://researchonline.lshtm.ac.uk/id/eprint/4003/ |
genre |
Arctic charr atlantic cod Atlantic salmon |
genre_facet |
Arctic charr atlantic cod Atlantic salmon |
op_relation |
Arnadottir, Helga; Hvanndal, Iris; Andresdottir, Valgerdur; Burr, Sarah E <https://researchonline.lshtm.ac.uk/view/creators/icrusbur.html>; Frey, Joachim; Gudmundsdottir, Bjarnheidur K; (2009) The AsaP1 peptidase of Aeromonas salmonicida subsp. achromogenes is a highly conserved deuterolysin metalloprotease (family M35) and a major virulence factor. Journal of bacteriology, 191 (1). pp. 403-410. ISSN 0021-9193 DOI: https://doi.org/10.1128/JB.00847-08 <https://doi.org/10.1128/JB.00847-08> |
op_doi |
https://doi.org/10.1128/JB.00847-08 |
container_title |
Journal of Bacteriology |
container_volume |
191 |
container_issue |
1 |
container_start_page |
403 |
op_container_end_page |
410 |
_version_ |
1810294450152275968 |