Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas
In the present study, a shape-independent differential scanning calorimeter (DSC) technique was used to measure the dehydration response during freezing of sperm cells from diploid and tetraploid Pacific oysters, Crassostrea gigas. This represents the first application of the DSC technique to sperm...
Published in: | Biology of Reproduction |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | unknown |
Published: |
LSU Digital Commons
2004
|
Subjects: | |
Online Access: | https://digitalcommons.lsu.edu/mechanical_engineering_pubs/665 https://doi.org/10.1095/biolreprod.103.025296 |
id |
ftlouisianastuir:oai:digitalcommons.lsu.edu:mechanical_engineering_pubs-1664 |
---|---|
record_format |
openpolar |
spelling |
ftlouisianastuir:oai:digitalcommons.lsu.edu:mechanical_engineering_pubs-1664 2023-06-11T04:11:08+02:00 Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas He, Yimeng Dong, Qiaoxiang Tiersch, Terrence R Devireddy, Ram V 2004-05-01T07:00:00Z https://digitalcommons.lsu.edu/mechanical_engineering_pubs/665 https://doi.org/10.1095/biolreprod.103.025296 unknown LSU Digital Commons https://digitalcommons.lsu.edu/mechanical_engineering_pubs/665 doi:10.1095/biolreprod.103.025296 Faculty Publications text 2004 ftlouisianastuir https://doi.org/10.1095/biolreprod.103.025296 2023-05-28T18:53:05Z In the present study, a shape-independent differential scanning calorimeter (DSC) technique was used to measure the dehydration response during freezing of sperm cells from diploid and tetraploid Pacific oysters, Crassostrea gigas. This represents the first application of the DSC technique to sperm cells from nonmammalian species. Volumetric shrinkage during freezing of oyster sperm cell suspensions was obtained at cooling rates of 5 and 20 degrees C/min in the presence of extracellular ice and 8% (v/v) concentration of dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA). Using previously published data, sperm cells from diploid oysters were modeled as a two-compartment "ball-on-stick" model with a "ball" 1.66 microm in diameter and a "stick" 41 microm in length and 0.14 microm wide. Similarly, sperm cells of tetraploid oysters were modeled with a "ball" 2.14 microm in diameter and a "stick" 53 microm in length and 0.17 microm wide. Sperm cells of both ploidy levels were assumed to have an osmotically inactive cell volume, Vb, of 0.6 Vo, where Vo is the isotonic (or initial) cell volume. By fitting a model of water transport to the experimentally obtained volumetric shrinkage data, the best-fit membrane permeability parameters (Lpg and ELp) were determined. The combined-best-fit membrane permeability parameters at 5 and 20 degrees C/min for haploid sperm cells (or cells from diploid Pacific oysters) in the absence of CPAs were: Lpg = 0.30 x 10(-15) m(3)/Ns (0.0017 microm/min-atm) and ELp = 41.0 kJ/mole (9.8 kcal/mole). The corresponding parameters in the presence of 8% DMSO were: Lpg[cpa] = 0.27 x 10(-15) m(3)/Ns (0.0015 microm/min-atm) and ELp[cpa] = 38.0 kJ/mole (9.1 kcal/mole). Similarly, the combined-best-fit membrane permeability parameters at 5 and 20 degrees C/min for diploid sperm cells (or cells from tetraploid Pacific oysters) in the absence of CPAs were: Lpg = 0.34 x 10(-15) m(3)/Ns (0.0019 microm/min-atm) and ELp = 29.7 kJ/mole (7.1 kcal/mole). The corresponding parameters in the ... Text Crassostrea gigas Pacific oyster LSU Digital Commons (Louisiana State University) Pacific Biology of Reproduction 70 5 1428 1437 |
institution |
Open Polar |
collection |
LSU Digital Commons (Louisiana State University) |
op_collection_id |
ftlouisianastuir |
language |
unknown |
description |
In the present study, a shape-independent differential scanning calorimeter (DSC) technique was used to measure the dehydration response during freezing of sperm cells from diploid and tetraploid Pacific oysters, Crassostrea gigas. This represents the first application of the DSC technique to sperm cells from nonmammalian species. Volumetric shrinkage during freezing of oyster sperm cell suspensions was obtained at cooling rates of 5 and 20 degrees C/min in the presence of extracellular ice and 8% (v/v) concentration of dimethyl sulfoxide (DMSO), a commonly used cryoprotective agent (CPA). Using previously published data, sperm cells from diploid oysters were modeled as a two-compartment "ball-on-stick" model with a "ball" 1.66 microm in diameter and a "stick" 41 microm in length and 0.14 microm wide. Similarly, sperm cells of tetraploid oysters were modeled with a "ball" 2.14 microm in diameter and a "stick" 53 microm in length and 0.17 microm wide. Sperm cells of both ploidy levels were assumed to have an osmotically inactive cell volume, Vb, of 0.6 Vo, where Vo is the isotonic (or initial) cell volume. By fitting a model of water transport to the experimentally obtained volumetric shrinkage data, the best-fit membrane permeability parameters (Lpg and ELp) were determined. The combined-best-fit membrane permeability parameters at 5 and 20 degrees C/min for haploid sperm cells (or cells from diploid Pacific oysters) in the absence of CPAs were: Lpg = 0.30 x 10(-15) m(3)/Ns (0.0017 microm/min-atm) and ELp = 41.0 kJ/mole (9.8 kcal/mole). The corresponding parameters in the presence of 8% DMSO were: Lpg[cpa] = 0.27 x 10(-15) m(3)/Ns (0.0015 microm/min-atm) and ELp[cpa] = 38.0 kJ/mole (9.1 kcal/mole). Similarly, the combined-best-fit membrane permeability parameters at 5 and 20 degrees C/min for diploid sperm cells (or cells from tetraploid Pacific oysters) in the absence of CPAs were: Lpg = 0.34 x 10(-15) m(3)/Ns (0.0019 microm/min-atm) and ELp = 29.7 kJ/mole (7.1 kcal/mole). The corresponding parameters in the ... |
format |
Text |
author |
He, Yimeng Dong, Qiaoxiang Tiersch, Terrence R Devireddy, Ram V |
spellingShingle |
He, Yimeng Dong, Qiaoxiang Tiersch, Terrence R Devireddy, Ram V Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
author_facet |
He, Yimeng Dong, Qiaoxiang Tiersch, Terrence R Devireddy, Ram V |
author_sort |
He, Yimeng |
title |
Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
title_short |
Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
title_full |
Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
title_fullStr |
Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid Pacific oyster, Crassostrea gigas |
title_sort |
variation in the membrane transport properties and predicted optimal rates of freezing for spermatozoa of diploid and tetraploid pacific oyster, crassostrea gigas |
publisher |
LSU Digital Commons |
publishDate |
2004 |
url |
https://digitalcommons.lsu.edu/mechanical_engineering_pubs/665 https://doi.org/10.1095/biolreprod.103.025296 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Faculty Publications |
op_relation |
https://digitalcommons.lsu.edu/mechanical_engineering_pubs/665 doi:10.1095/biolreprod.103.025296 |
op_doi |
https://doi.org/10.1095/biolreprod.103.025296 |
container_title |
Biology of Reproduction |
container_volume |
70 |
container_issue |
5 |
container_start_page |
1428 |
op_container_end_page |
1437 |
_version_ |
1768386016348995584 |