Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus).
A growth hormone (GH) gene was isolated from nuclear DNA of the red drum (Sciaenops ocellatus). Synthetic oligonucleotides were synthesized based on published cDNA sequences of other perciform fishes. Polymerase chain reaction (PCR) methodology was employed to amplify a nucleotide sequence extending...
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1991
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ftlouisianastuir:oai:digitalcommons.lsu.edu:gradschool_disstheses-6114 2023-06-11T04:16:16+02:00 Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). Dorworth, Carol Marie 1991-01-01T08:00:00Z application/pdf https://digitalcommons.lsu.edu/gradschool_disstheses/5115 https://doi.org/10.31390/gradschool_disstheses.5115 https://digitalcommons.lsu.edu/context/gradschool_disstheses/article/6114/viewcontent/9200058.pdf English eng LSU Digital Commons https://digitalcommons.lsu.edu/gradschool_disstheses/5115 doi:10.31390/gradschool_disstheses.5115 https://digitalcommons.lsu.edu/context/gradschool_disstheses/article/6114/viewcontent/9200058.pdf LSU Historical Dissertations and Theses Agriculture forestry and wildlife fisheries and aquaculture text 1991 ftlouisianastuir https://doi.org/10.31390/gradschool_disstheses.5115 2023-05-28T19:14:20Z A growth hormone (GH) gene was isolated from nuclear DNA of the red drum (Sciaenops ocellatus). Synthetic oligonucleotides were synthesized based on published cDNA sequences of other perciform fishes. Polymerase chain reaction (PCR) methodology was employed to amplify a nucleotide sequence extending from the 5$\sp\prime$ end of intron I to 15 bases upstream of the 3$\sp\prime$ end of exon VI of the GH gene. Amplified DNA was cloned into a phagemid sequencing vector. Clones were sequenced. Computer analysis of sequence data was used to determine the internal gene arrangement of exons and introns. Homologies of the nucleotide and deduced amino acid sequences with published GH sequences were determined. The GH nucleotide sequence was examined for the presence of regulatory elements. The red drum GH gene is comprised of six exons and five introns, an arrangement like that of the salmonid GH genes but unlike the five exon, four intron arrangement of carp (Cyprinus carpio) and mammalian GH genes. Protein coding regions of the red drum GH gene show a high degree of homology with GH cDNA sequences of other perciform fishes. The 3$\sp\prime$ terminus of exon V of the red drum GH gene shows an 86% similarity with a region of exon V of the human chorionic somatomammotropin gene. A putative glucocorticoid receptor element is present in intron I of the red drum GH gene. Text Red drum Sciaenops ocellatus LSU Digital Commons (Louisiana State University) |
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LSU Digital Commons (Louisiana State University) |
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English |
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Agriculture forestry and wildlife fisheries and aquaculture |
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Agriculture forestry and wildlife fisheries and aquaculture Dorworth, Carol Marie Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
topic_facet |
Agriculture forestry and wildlife fisheries and aquaculture |
description |
A growth hormone (GH) gene was isolated from nuclear DNA of the red drum (Sciaenops ocellatus). Synthetic oligonucleotides were synthesized based on published cDNA sequences of other perciform fishes. Polymerase chain reaction (PCR) methodology was employed to amplify a nucleotide sequence extending from the 5$\sp\prime$ end of intron I to 15 bases upstream of the 3$\sp\prime$ end of exon VI of the GH gene. Amplified DNA was cloned into a phagemid sequencing vector. Clones were sequenced. Computer analysis of sequence data was used to determine the internal gene arrangement of exons and introns. Homologies of the nucleotide and deduced amino acid sequences with published GH sequences were determined. The GH nucleotide sequence was examined for the presence of regulatory elements. The red drum GH gene is comprised of six exons and five introns, an arrangement like that of the salmonid GH genes but unlike the five exon, four intron arrangement of carp (Cyprinus carpio) and mammalian GH genes. Protein coding regions of the red drum GH gene show a high degree of homology with GH cDNA sequences of other perciform fishes. The 3$\sp\prime$ terminus of exon V of the red drum GH gene shows an 86% similarity with a region of exon V of the human chorionic somatomammotropin gene. A putative glucocorticoid receptor element is present in intron I of the red drum GH gene. |
format |
Text |
author |
Dorworth, Carol Marie |
author_facet |
Dorworth, Carol Marie |
author_sort |
Dorworth, Carol Marie |
title |
Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
title_short |
Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
title_full |
Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
title_fullStr |
Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
title_full_unstemmed |
Partial Sequence and Characterization of a Growth Hormone Gene of the Red Drum (Sciaenops Ocellatus). |
title_sort |
partial sequence and characterization of a growth hormone gene of the red drum (sciaenops ocellatus). |
publisher |
LSU Digital Commons |
publishDate |
1991 |
url |
https://digitalcommons.lsu.edu/gradschool_disstheses/5115 https://doi.org/10.31390/gradschool_disstheses.5115 https://digitalcommons.lsu.edu/context/gradschool_disstheses/article/6114/viewcontent/9200058.pdf |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
LSU Historical Dissertations and Theses |
op_relation |
https://digitalcommons.lsu.edu/gradschool_disstheses/5115 doi:10.31390/gradschool_disstheses.5115 https://digitalcommons.lsu.edu/context/gradschool_disstheses/article/6114/viewcontent/9200058.pdf |
op_doi |
https://doi.org/10.31390/gradschool_disstheses.5115 |
_version_ |
1768373862613909504 |