Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica
The anoxic and freezing brine that permeates Lake Vida's perennial ice below 16mcontains an abundance of very small (≤0.2-μm) particles mixed with a less abundant population of microbial cells ranging from>0.2 to 1.5 μmin length. Fluorescent DNA staining, electron microscopy (EM) observation...
Published in: | Applied and Environmental Microbiology |
---|---|
Main Authors: | , , , , , , |
Format: | Text |
Language: | unknown |
Published: |
LSU Digital Commons
2014
|
Subjects: | |
Online Access: | https://digitalcommons.lsu.edu/geo_pubs/616 https://doi.org/10.1128/AEM.00276-14 https://digitalcommons.lsu.edu/context/geo_pubs/article/1615/viewcontent/616.pdf |
id |
ftlouisianastuir:oai:digitalcommons.lsu.edu:geo_pubs-1615 |
---|---|
record_format |
openpolar |
spelling |
ftlouisianastuir:oai:digitalcommons.lsu.edu:geo_pubs-1615 2023-06-11T04:04:40+02:00 Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica Kuhn, Emanuele Ichimura, Andrew S. Peng, Vivian Fritsen, Christian H. Trubl, Gareth Doran, Peter T. Murray, Alison E. 2014-01-01T08:00:00Z application/pdf https://digitalcommons.lsu.edu/geo_pubs/616 https://doi.org/10.1128/AEM.00276-14 https://digitalcommons.lsu.edu/context/geo_pubs/article/1615/viewcontent/616.pdf unknown LSU Digital Commons https://digitalcommons.lsu.edu/geo_pubs/616 doi:10.1128/AEM.00276-14 https://digitalcommons.lsu.edu/context/geo_pubs/article/1615/viewcontent/616.pdf Faculty Publications text 2014 ftlouisianastuir https://doi.org/10.1128/AEM.00276-14 2023-05-28T18:24:25Z The anoxic and freezing brine that permeates Lake Vida's perennial ice below 16mcontains an abundance of very small (≤0.2-μm) particles mixed with a less abundant population of microbial cells ranging from>0.2 to 1.5 μmin length. Fluorescent DNA staining, electron microscopy (EM) observations, elemental analysis, and extraction of high-molecular-weight genomic DNA indicated that a significant portion of these ultrasmall particles are cells. A continuous electron-dense layer surrounding a less electron-dense region was observed by EM, indicating the presence of a biological membrane surrounding a cytoplasm. The ultrasmall cells are 0.192±0.065 μ, with morphology characteristic of coccoid and diplococcic bacterial cells, often surrounded by iron-rich capsular structures. EM observations also detected the presence of smaller unidentified nanoparticles of 0.020 to 0.140 μmamong the brine cells. A 16S rRNA gene clone library from the brine 0.1- to 0.2-μm-size fraction revealed a relatively low-diversity assemblage of Bacteria sequences distinct from the previously reported>0.2-μm-cell-size Lake Vida brine assemblage. The brine 0.1- to 0.2-μm-size fraction was dominated by the Proteobacteria-affiliated genera Herbaspirillum, Pseudoalteromonas, and Marinobacter. Cultivation efforts of the 0.1- to 0.2-μm-size fraction led to the isolation of Actinobacteria-affiliated genera Microbacterium and Kocuria. Based on phylogenetic relatedness and microscopic observations, we hypothesize that the ultrasmall cells in Lake Vida brine are ultramicrocells that are likely in a reduced size state as a result of environmental stress or life cycle-related conditions. © 2014, American Society for Microbiology. Text Antarc* Antarctica LSU Digital Commons (Louisiana State University) Lake Vida ENVELOPE(161.950,161.950,-77.383,-77.383) Applied and Environmental Microbiology 80 12 3687 3698 |
institution |
Open Polar |
collection |
LSU Digital Commons (Louisiana State University) |
op_collection_id |
ftlouisianastuir |
language |
unknown |
description |
The anoxic and freezing brine that permeates Lake Vida's perennial ice below 16mcontains an abundance of very small (≤0.2-μm) particles mixed with a less abundant population of microbial cells ranging from>0.2 to 1.5 μmin length. Fluorescent DNA staining, electron microscopy (EM) observations, elemental analysis, and extraction of high-molecular-weight genomic DNA indicated that a significant portion of these ultrasmall particles are cells. A continuous electron-dense layer surrounding a less electron-dense region was observed by EM, indicating the presence of a biological membrane surrounding a cytoplasm. The ultrasmall cells are 0.192±0.065 μ, with morphology characteristic of coccoid and diplococcic bacterial cells, often surrounded by iron-rich capsular structures. EM observations also detected the presence of smaller unidentified nanoparticles of 0.020 to 0.140 μmamong the brine cells. A 16S rRNA gene clone library from the brine 0.1- to 0.2-μm-size fraction revealed a relatively low-diversity assemblage of Bacteria sequences distinct from the previously reported>0.2-μm-cell-size Lake Vida brine assemblage. The brine 0.1- to 0.2-μm-size fraction was dominated by the Proteobacteria-affiliated genera Herbaspirillum, Pseudoalteromonas, and Marinobacter. Cultivation efforts of the 0.1- to 0.2-μm-size fraction led to the isolation of Actinobacteria-affiliated genera Microbacterium and Kocuria. Based on phylogenetic relatedness and microscopic observations, we hypothesize that the ultrasmall cells in Lake Vida brine are ultramicrocells that are likely in a reduced size state as a result of environmental stress or life cycle-related conditions. © 2014, American Society for Microbiology. |
format |
Text |
author |
Kuhn, Emanuele Ichimura, Andrew S. Peng, Vivian Fritsen, Christian H. Trubl, Gareth Doran, Peter T. Murray, Alison E. |
spellingShingle |
Kuhn, Emanuele Ichimura, Andrew S. Peng, Vivian Fritsen, Christian H. Trubl, Gareth Doran, Peter T. Murray, Alison E. Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
author_facet |
Kuhn, Emanuele Ichimura, Andrew S. Peng, Vivian Fritsen, Christian H. Trubl, Gareth Doran, Peter T. Murray, Alison E. |
author_sort |
Kuhn, Emanuele |
title |
Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
title_short |
Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
title_full |
Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
title_fullStr |
Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
title_full_unstemmed |
Brine assemblages of ultrasmall microbial cells within the ice cover of Lake Vida, Antarctica |
title_sort |
brine assemblages of ultrasmall microbial cells within the ice cover of lake vida, antarctica |
publisher |
LSU Digital Commons |
publishDate |
2014 |
url |
https://digitalcommons.lsu.edu/geo_pubs/616 https://doi.org/10.1128/AEM.00276-14 https://digitalcommons.lsu.edu/context/geo_pubs/article/1615/viewcontent/616.pdf |
long_lat |
ENVELOPE(161.950,161.950,-77.383,-77.383) |
geographic |
Lake Vida |
geographic_facet |
Lake Vida |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Faculty Publications |
op_relation |
https://digitalcommons.lsu.edu/geo_pubs/616 doi:10.1128/AEM.00276-14 https://digitalcommons.lsu.edu/context/geo_pubs/article/1615/viewcontent/616.pdf |
op_doi |
https://doi.org/10.1128/AEM.00276-14 |
container_title |
Applied and Environmental Microbiology |
container_volume |
80 |
container_issue |
12 |
container_start_page |
3687 |
op_container_end_page |
3698 |
_version_ |
1768389596678193152 |