Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica
© 2018 Elsevier Ltd Lake Vida, located in a closed basin in the McMurdo Dry Valleys, East Antarctica, permanently encapsulates an interstitial anoxic, aphotic, cold (−13 °C), brine ecosystem within 27+ m of ice. Metabolically active, but cold-limited, slow-growing bacteria were detected in the brine...
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ftlouisianastuir:oai:digitalcommons.lsu.edu:geo_pubs-1587 2023-06-11T04:04:40+02:00 Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica Chou, Luoth Kenig, Fabien Murray, Alison E. Fritsen, Christian H. Doran, Peter T. 2018-08-01T07:00:00Z https://digitalcommons.lsu.edu/geo_pubs/588 https://doi.org/10.1016/j.orggeochem.2018.05.002 unknown LSU Digital Commons https://digitalcommons.lsu.edu/geo_pubs/588 doi:10.1016/j.orggeochem.2018.05.002 Faculty Publications Brine Cryosphere Environmental metabolomics Geomicrobiology Legacy Limnology Paleometabolites text 2018 ftlouisianastuir https://doi.org/10.1016/j.orggeochem.2018.05.002 2023-05-28T18:16:59Z © 2018 Elsevier Ltd Lake Vida, located in a closed basin in the McMurdo Dry Valleys, East Antarctica, permanently encapsulates an interstitial anoxic, aphotic, cold (−13 °C), brine ecosystem within 27+ m of ice. Metabolically active, but cold-limited, slow-growing bacteria were detected in the brine. Lake Vida brine is derived from the evaporation of a body of water that occupied the same basin prior to ∼2800 years ago. The characteristics of this body of water changed over time and, at one point, likely resembled other modern well-studied perennial ice-covered lakes of the Dry Valleys. We characterized the dichloromethane-extractable fraction of the environmental metabolome of Lake Vida brine in order to constrain current and ancient biogeochemical processes. Analysis of the dichloromethane-extract of Lake Vida brine by gas chromatography-mass spectrometry and comprehensive multidimensional gas chromatography-time of flight-mass spectrometry reveals the presence of legacy compounds (i.e. diagenetic products of chlorophylls and carotenoids) deriving from photosynthetic algae and anaerobic, anoxygenic photosynthetic bacteria. This legacy component dilutes the environmental signal of metabolites deriving from the extant bacterial community. The persistence of legacy metabolites (paleometabolites), apparent in Lake Vida brine, is a result of the slow turnover rates of the extant bacterial population due to low metabolic activities caused by the cold limitation. Such paleometabolites may also be preserved in other cold-limited or nutrient-depleted slow-growing ecosystems. When analyzing ecosystems with low metabolic rates, the presence of legacy metabolites must first be addressed in order to confidently recognize and interpret the environmental metabolome of the extant ecosystem. Text Antarc* Antarctica East Antarctica McMurdo Dry Valleys LSU Digital Commons (Louisiana State University) East Antarctica McMurdo Dry Valleys Lake Vida ENVELOPE(161.950,161.950,-77.383,-77.383) Organic Geochemistry 122 161 170 |
institution |
Open Polar |
collection |
LSU Digital Commons (Louisiana State University) |
op_collection_id |
ftlouisianastuir |
language |
unknown |
topic |
Brine Cryosphere Environmental metabolomics Geomicrobiology Legacy Limnology Paleometabolites |
spellingShingle |
Brine Cryosphere Environmental metabolomics Geomicrobiology Legacy Limnology Paleometabolites Chou, Luoth Kenig, Fabien Murray, Alison E. Fritsen, Christian H. Doran, Peter T. Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
topic_facet |
Brine Cryosphere Environmental metabolomics Geomicrobiology Legacy Limnology Paleometabolites |
description |
© 2018 Elsevier Ltd Lake Vida, located in a closed basin in the McMurdo Dry Valleys, East Antarctica, permanently encapsulates an interstitial anoxic, aphotic, cold (−13 °C), brine ecosystem within 27+ m of ice. Metabolically active, but cold-limited, slow-growing bacteria were detected in the brine. Lake Vida brine is derived from the evaporation of a body of water that occupied the same basin prior to ∼2800 years ago. The characteristics of this body of water changed over time and, at one point, likely resembled other modern well-studied perennial ice-covered lakes of the Dry Valleys. We characterized the dichloromethane-extractable fraction of the environmental metabolome of Lake Vida brine in order to constrain current and ancient biogeochemical processes. Analysis of the dichloromethane-extract of Lake Vida brine by gas chromatography-mass spectrometry and comprehensive multidimensional gas chromatography-time of flight-mass spectrometry reveals the presence of legacy compounds (i.e. diagenetic products of chlorophylls and carotenoids) deriving from photosynthetic algae and anaerobic, anoxygenic photosynthetic bacteria. This legacy component dilutes the environmental signal of metabolites deriving from the extant bacterial community. The persistence of legacy metabolites (paleometabolites), apparent in Lake Vida brine, is a result of the slow turnover rates of the extant bacterial population due to low metabolic activities caused by the cold limitation. Such paleometabolites may also be preserved in other cold-limited or nutrient-depleted slow-growing ecosystems. When analyzing ecosystems with low metabolic rates, the presence of legacy metabolites must first be addressed in order to confidently recognize and interpret the environmental metabolome of the extant ecosystem. |
format |
Text |
author |
Chou, Luoth Kenig, Fabien Murray, Alison E. Fritsen, Christian H. Doran, Peter T. |
author_facet |
Chou, Luoth Kenig, Fabien Murray, Alison E. Fritsen, Christian H. Doran, Peter T. |
author_sort |
Chou, Luoth |
title |
Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
title_short |
Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
title_full |
Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
title_fullStr |
Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
title_full_unstemmed |
Effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of Lake Vida, East Antarctica |
title_sort |
effects of legacy metabolites from previous ecosystems on the environmental metabolomics of the brine of lake vida, east antarctica |
publisher |
LSU Digital Commons |
publishDate |
2018 |
url |
https://digitalcommons.lsu.edu/geo_pubs/588 https://doi.org/10.1016/j.orggeochem.2018.05.002 |
long_lat |
ENVELOPE(161.950,161.950,-77.383,-77.383) |
geographic |
East Antarctica McMurdo Dry Valleys Lake Vida |
geographic_facet |
East Antarctica McMurdo Dry Valleys Lake Vida |
genre |
Antarc* Antarctica East Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctica East Antarctica McMurdo Dry Valleys |
op_source |
Faculty Publications |
op_relation |
https://digitalcommons.lsu.edu/geo_pubs/588 doi:10.1016/j.orggeochem.2018.05.002 |
op_doi |
https://doi.org/10.1016/j.orggeochem.2018.05.002 |
container_title |
Organic Geochemistry |
container_volume |
122 |
container_start_page |
161 |
op_container_end_page |
170 |
_version_ |
1768389584445505536 |