Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters
The plasma of eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters were compared for levels of inhibitory activities against a variety of proteases. Representatives of the serine, cysteine, metallo and aspartic protease mechanistic classes were analyzed, including extracellular pr...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | unknown |
Published: |
LSU Digital Commons
1998
|
Subjects: | |
Online Access: | https://digitalcommons.lsu.edu/animalsciences_pubs/841 https://doi.org/10.1016/S0305-0491(98)10084-6 |
id |
ftlouisianastuir:oai:digitalcommons.lsu.edu:animalsciences_pubs-1840 |
---|---|
record_format |
openpolar |
spelling |
ftlouisianastuir:oai:digitalcommons.lsu.edu:animalsciences_pubs-1840 2023-06-11T04:11:04+02:00 Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters Faisal, M. MacIntyre, E. A. Adham, K. G. Tall, B. D. Kothary, M. H. La Peyre, J. F. 1998-01-01T08:00:00Z https://digitalcommons.lsu.edu/animalsciences_pubs/841 https://doi.org/10.1016/S0305-0491(98)10084-6 unknown LSU Digital Commons https://digitalcommons.lsu.edu/animalsciences_pubs/841 doi:10.1016/S0305-0491(98)10084-6 Faculty Publications Oyster Perkinsus marinus Protease inhibitors Vibrio vulnificus text 1998 ftlouisianastuir https://doi.org/10.1016/S0305-0491(98)10084-6 2023-05-28T18:12:50Z The plasma of eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters were compared for levels of inhibitory activities against a variety of proteases. Representatives of the serine, cysteine, metallo and aspartic protease mechanistic classes were analyzed, including extracellular proteases produced by two oyster-associated pathogens; Perkinsus marinus and Vibrio vulnificus. In comparison to C. virginica, C. gigas plasma exhibited significantly higher specific inhibition levels (ng protease inhibited/μg plasma protein) for papain (P<0.001), pepsin (P<0.001), P. marinus protease (P<0.001), trypsin (P=0.015), and V. vulnificus protease (P<0.001). Plasma of C. gigas did not inhibit the metalloprotease thermolysin. Instead, a significant increase in substrate hydrolysis was seen in wells containing plasma and thermolysin in comparison to wells containing thermolysin only. A similar trend was noted for thermolysin with the eastern oyster samples. These studies indicate the presence of protease inhibitors in the plasma of Crassostrea spp., which may have an impact upon host defense mechanisms, in addition to other physiological roles. Copyright (C) 1998 Elsevier Science Inc. Text Crassostrea gigas LSU Digital Commons (Louisiana State University) Pacific Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 121 2 161 168 |
institution |
Open Polar |
collection |
LSU Digital Commons (Louisiana State University) |
op_collection_id |
ftlouisianastuir |
language |
unknown |
topic |
Oyster Perkinsus marinus Protease inhibitors Vibrio vulnificus |
spellingShingle |
Oyster Perkinsus marinus Protease inhibitors Vibrio vulnificus Faisal, M. MacIntyre, E. A. Adham, K. G. Tall, B. D. Kothary, M. H. La Peyre, J. F. Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
topic_facet |
Oyster Perkinsus marinus Protease inhibitors Vibrio vulnificus |
description |
The plasma of eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters were compared for levels of inhibitory activities against a variety of proteases. Representatives of the serine, cysteine, metallo and aspartic protease mechanistic classes were analyzed, including extracellular proteases produced by two oyster-associated pathogens; Perkinsus marinus and Vibrio vulnificus. In comparison to C. virginica, C. gigas plasma exhibited significantly higher specific inhibition levels (ng protease inhibited/μg plasma protein) for papain (P<0.001), pepsin (P<0.001), P. marinus protease (P<0.001), trypsin (P=0.015), and V. vulnificus protease (P<0.001). Plasma of C. gigas did not inhibit the metalloprotease thermolysin. Instead, a significant increase in substrate hydrolysis was seen in wells containing plasma and thermolysin in comparison to wells containing thermolysin only. A similar trend was noted for thermolysin with the eastern oyster samples. These studies indicate the presence of protease inhibitors in the plasma of Crassostrea spp., which may have an impact upon host defense mechanisms, in addition to other physiological roles. Copyright (C) 1998 Elsevier Science Inc. |
format |
Text |
author |
Faisal, M. MacIntyre, E. A. Adham, K. G. Tall, B. D. Kothary, M. H. La Peyre, J. F. |
author_facet |
Faisal, M. MacIntyre, E. A. Adham, K. G. Tall, B. D. Kothary, M. H. La Peyre, J. F. |
author_sort |
Faisal, M. |
title |
Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
title_short |
Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
title_full |
Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
title_fullStr |
Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
title_full_unstemmed |
Evidence for the presence of protease inhibitors in eastern (Crassostrea virginica) and Pacific (Crassostrea gigas) oysters |
title_sort |
evidence for the presence of protease inhibitors in eastern (crassostrea virginica) and pacific (crassostrea gigas) oysters |
publisher |
LSU Digital Commons |
publishDate |
1998 |
url |
https://digitalcommons.lsu.edu/animalsciences_pubs/841 https://doi.org/10.1016/S0305-0491(98)10084-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Faculty Publications |
op_relation |
https://digitalcommons.lsu.edu/animalsciences_pubs/841 doi:10.1016/S0305-0491(98)10084-6 |
op_doi |
https://doi.org/10.1016/S0305-0491(98)10084-6 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
121 |
container_issue |
2 |
container_start_page |
161 |
op_container_end_page |
168 |
_version_ |
1768385906795872256 |