Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T
Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet....
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Online Access: | http://hdl.handle.net/10400.9/2925 https://doi.org/10.1016/j.enzmictec.2014.10.008 |
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ftlneg:oai:repositorio.lneg.pt:10400.9/2925 2023-05-15T14:02:32+02:00 Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo 2016-04-11T10:16:12Z http://hdl.handle.net/10400.9/2925 https://doi.org/10.1016/j.enzmictec.2014.10.008 eng eng Elsevier FCOMP-01-0124-FEDER-041191 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/133632/PT http://dx.doi.org/10.1016/j.enzmictec.2014.10.008 Faria, N.T.; Marques, S.; Fonseca, C.; Ferreira, F.C. - Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T. In: Enzyme and Microbial Technology, 2015, Vol. 71, p. 58-65 0141-0229 http://hdl.handle.net/10400.9/2925 doi:10.1016/j.enzmictec.2014.10.008 openAccess Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp Cellulosic materials article 2016 ftlneg https://doi.org/10.1016/j.enzmictec.2014.10.008 2022-12-14T01:03:13Z Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production. Article in Journal/Newspaper Antarc* Antarctica Laboratório Nacional de Energia e Geologia: Repositório Científico do LNEG Enzyme and Microbial Technology 71 58 65 |
institution |
Open Polar |
collection |
Laboratório Nacional de Energia e Geologia: Repositório Científico do LNEG |
op_collection_id |
ftlneg |
language |
English |
topic |
Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp Cellulosic materials |
spellingShingle |
Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp Cellulosic materials Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
topic_facet |
Consolidated bioprocessing Lipids Xylan Cellulose Pseudozyma spp Cellulosic materials |
description |
Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and xylan (beechwood) as carbon and energy source has been assessed along with their capacity of producing cellulolytic and hemicellulolytic enzymes, toward a consolidated bioprocess (CBP) for MEL production. The yeasts assessed were neither able to grow in medium containing Avicel® nor produce cellulolytic enzymes under the conditions tested. On contrary, both yeasts were able to efficiently grow in xylan, but MEL production was only detected in P. antarctica PYCC 5048T cultures. MEL titers reached 1.3 g/l after 10 days in batch cultures with 40 g/l xylan, and 2.0 g/l in fed-batch cultures with xylan feeding (additional 40 g/l) at day 4. High levels of xylanase activities were detected in xylan cultures, reaching 47–62 U/ml (31–32 U/mg) at 50 °C, and still exhibiting more than 10 U/ml under physiological temperature (28 °C). Total ß-xylosidase activities, displayed mainly as wall-bounded and extracellular activity, accounted for 0.154 and 0.176 U/ml in P. antarctica PYCC 5048T and P. aphidis PYCC 5535T cultures, respectively. The present results demonstrate the potential of Pseudozyma spp. for using directly a fraction of lignocellulosic biomass, xylan, and combining in the same bioprocess the production of xylanolytic enzymes with MEL production. |
format |
Article in Journal/Newspaper |
author |
Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo |
author_facet |
Faria, Nuno Torres Marques, Susana Fonseca, César Ferreira, Frederico Castelo |
author_sort |
Faria, Nuno Torres |
title |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_short |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_full |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_fullStr |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_full_unstemmed |
Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T |
title_sort |
direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by pseudozyma antarctica pycc 5048t |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/10400.9/2925 https://doi.org/10.1016/j.enzmictec.2014.10.008 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
FCOMP-01-0124-FEDER-041191 info:eu-repo/grantAgreement/FCT/5876-PPCDTI/133632/PT http://dx.doi.org/10.1016/j.enzmictec.2014.10.008 Faria, N.T.; Marques, S.; Fonseca, C.; Ferreira, F.C. - Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipids, by Pseudozyma antarctica PYCC 5048T. In: Enzyme and Microbial Technology, 2015, Vol. 71, p. 58-65 0141-0229 http://hdl.handle.net/10400.9/2925 doi:10.1016/j.enzmictec.2014.10.008 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.enzmictec.2014.10.008 |
container_title |
Enzyme and Microbial Technology |
container_volume |
71 |
container_start_page |
58 |
op_container_end_page |
65 |
_version_ |
1766272846232813568 |