Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains
The pentose-assimilating capacity of yeasts from the genus Pseudozyma, P. antarctica PYCC 5048T, P.aphidis PYCC 5535Tand P. rugulosa PYCC 5537T, was exploited towards the production of mannosylery-thritol lipids (MEL), a glycolipid with biosurfactant properties. The three strains tested were able to...
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ftlneg:oai:repositorio.lneg.pt:10400.9/2580 2023-05-15T14:02:32+02:00 Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains Faria, Nuno Torres Santos, Marisa V. Fernandes, Pedro Fonseca, Luís Lopes Fonseca, César Ferreira, Frederico Castelo 2015-01-22T11:33:00Z http://hdl.handle.net/10400.9/2580 eng eng Elsevier http://dx.doi.org/10.1016/j.procbio.2014.08.004 Faria, N.T.; Santos, M.V.; Fernandes, P.; Fonseca, L.L.; Fonseca, C.; Ferreira, F.C. - Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains. In: Process Biochemistry, 2014, Vol. 49, p. 1790-1799 1359-5113 http://hdl.handle.net/10400.9/2580 openAccess Lipids Biosurfactants Pentoses Xylose Pseudozyma spp article 2015 ftlneg https://doi.org/10.1016/j.procbio.2014.08.004 2022-08-03T00:02:51Z The pentose-assimilating capacity of yeasts from the genus Pseudozyma, P. antarctica PYCC 5048T, P.aphidis PYCC 5535Tand P. rugulosa PYCC 5537T, was exploited towards the production of mannosylery-thritol lipids (MEL), a glycolipid with biosurfactant properties. The three strains tested were able togrow on d-xylose and l-arabinose with similar maximum specific growth rates to those estimated on d-glucose (around 0.2 h-1). The highest MEL titres (4.8–5.4 g/l) and yields (0.11–0.14 g/g) from d-xylosewere found in P. antarctica PYCC 5048T, which presented similar values to those estimated on D-glucose and on D-xylose/D-glucose mixtures. P. rugulosa PYCC 5537T showed a pattern of sugar conversion into MEL similar to P. antarctica, but at 40% lower titres. P. aphidis PYCC 5535T presented lower MEL titres from D-xylose (1.2 g/l) than from D-glucose (3.4 g/l). Nitrate supply increased sugar consumption rate and, when accompanied by D-glucose or D-xylose feeding, additional biomass production. In this case, sugar was completely consumed before sugar feeding at day 7, but not when feeding is performed at day 4. Higher MEL titres where obtained for the later condition reaching 7.3 g/l and 5.8 g/l, in fed-atchcultures with glucose and xylose, respectively. Article in Journal/Newspaper Antarc* Antarctica Laboratório Nacional de Energia e Geologia: Repositório Científico do LNEG Process Biochemistry 49 11 1790 1799 |
institution |
Open Polar |
collection |
Laboratório Nacional de Energia e Geologia: Repositório Científico do LNEG |
op_collection_id |
ftlneg |
language |
English |
topic |
Lipids Biosurfactants Pentoses Xylose Pseudozyma spp |
spellingShingle |
Lipids Biosurfactants Pentoses Xylose Pseudozyma spp Faria, Nuno Torres Santos, Marisa V. Fernandes, Pedro Fonseca, Luís Lopes Fonseca, César Ferreira, Frederico Castelo Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
topic_facet |
Lipids Biosurfactants Pentoses Xylose Pseudozyma spp |
description |
The pentose-assimilating capacity of yeasts from the genus Pseudozyma, P. antarctica PYCC 5048T, P.aphidis PYCC 5535Tand P. rugulosa PYCC 5537T, was exploited towards the production of mannosylery-thritol lipids (MEL), a glycolipid with biosurfactant properties. The three strains tested were able togrow on d-xylose and l-arabinose with similar maximum specific growth rates to those estimated on d-glucose (around 0.2 h-1). The highest MEL titres (4.8–5.4 g/l) and yields (0.11–0.14 g/g) from d-xylosewere found in P. antarctica PYCC 5048T, which presented similar values to those estimated on D-glucose and on D-xylose/D-glucose mixtures. P. rugulosa PYCC 5537T showed a pattern of sugar conversion into MEL similar to P. antarctica, but at 40% lower titres. P. aphidis PYCC 5535T presented lower MEL titres from D-xylose (1.2 g/l) than from D-glucose (3.4 g/l). Nitrate supply increased sugar consumption rate and, when accompanied by D-glucose or D-xylose feeding, additional biomass production. In this case, sugar was completely consumed before sugar feeding at day 7, but not when feeding is performed at day 4. Higher MEL titres where obtained for the later condition reaching 7.3 g/l and 5.8 g/l, in fed-atchcultures with glucose and xylose, respectively. |
format |
Article in Journal/Newspaper |
author |
Faria, Nuno Torres Santos, Marisa V. Fernandes, Pedro Fonseca, Luís Lopes Fonseca, César Ferreira, Frederico Castelo |
author_facet |
Faria, Nuno Torres Santos, Marisa V. Fernandes, Pedro Fonseca, Luís Lopes Fonseca, César Ferreira, Frederico Castelo |
author_sort |
Faria, Nuno Torres |
title |
Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
title_short |
Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
title_full |
Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
title_fullStr |
Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
title_full_unstemmed |
Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains |
title_sort |
production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and d-glucose/d-xylose mixtures by pseudozyma yeast strains |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/10400.9/2580 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://dx.doi.org/10.1016/j.procbio.2014.08.004 Faria, N.T.; Santos, M.V.; Fernandes, P.; Fonseca, L.L.; Fonseca, C.; Ferreira, F.C. - Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentoses and D-glucose/D-xylose mixtures by Pseudozyma yeast strains. In: Process Biochemistry, 2014, Vol. 49, p. 1790-1799 1359-5113 http://hdl.handle.net/10400.9/2580 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1016/j.procbio.2014.08.004 |
container_title |
Process Biochemistry |
container_volume |
49 |
container_issue |
11 |
container_start_page |
1790 |
op_container_end_page |
1799 |
_version_ |
1766272845723205632 |