The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process
Atlantic cod (Gadus morhua) is a commercially important species of white fish, and one of three species legally identifiable as cod in the UK. Mislabelling of G. morhua does occur, as does the substitution of G. morhua for less expensive species. Sensitive molecular tests based on PCR have been deve...
Published in: | Food Control |
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Online Access: | http://researchonline.ljmu.ac.uk/id/eprint/7559/ https://researchonline.ljmu.ac.uk/id/eprint/7559/3/The%20detection%20of%20Atlantic%20cod%20%28Gadus%20morhua%29%20using%20loop%20mediated%20isothermal%20amplification%20in%20conjunction%20with%20a%20simplified%20DNA%20extraction%20process.pdf https://doi.org/10.1016/j.foodcont.2015.05.038 |
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ftliverpooljmu:oai:researchonline.ljmu.ac.uk:7559 2023-05-15T15:27:14+02:00 The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process Saull, J Duggan, C Hobbs, G Edwards, T 2015-06-03 text http://researchonline.ljmu.ac.uk/id/eprint/7559/ https://researchonline.ljmu.ac.uk/id/eprint/7559/3/The%20detection%20of%20Atlantic%20cod%20%28Gadus%20morhua%29%20using%20loop%20mediated%20isothermal%20amplification%20in%20conjunction%20with%20a%20simplified%20DNA%20extraction%20process.pdf https://doi.org/10.1016/j.foodcont.2015.05.038 en eng Elsevier https://researchonline.ljmu.ac.uk/id/eprint/7559/3/The%20detection%20of%20Atlantic%20cod%20%28Gadus%20morhua%29%20using%20loop%20mediated%20isothermal%20amplification%20in%20conjunction%20with%20a%20simplified%20DNA%20extraction%20process.pdf Saull, J, Duggan, C, Hobbs, G and Edwards, T (2015) The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process. Food Control, 59. pp. 306-313. ISSN 0956-7135 doi:10.1016/j.foodcont.2015.05.038 cc_by_nc_nd CC-BY-NC-ND TX341 Nutrition. Foods and food supply QH426 Genetics SH Aquaculture. Fisheries. Angling Article PeerReviewed 2015 ftliverpooljmu https://doi.org/10.1016/j.foodcont.2015.05.038 2022-01-09T06:53:30Z Atlantic cod (Gadus morhua) is a commercially important species of white fish, and one of three species legally identifiable as cod in the UK. Mislabelling of G. morhua does occur, as does the substitution of G. morhua for less expensive species. Sensitive molecular tests based on PCR have been developed for this species, but they have limitations, including the need for expensive thermal cycling equipment, and complex DNA extraction procedures. A loop mediated isothermal amplification (LAMP) assay was designed for the G. morhua cytochrome b gene, which was capable of detecting 0.1% w/w G. morhua in a homogenised raw fish mix. The LAMP assay was also able to detect G. morhua DNA when a rapid sample preparation was used, involving heating 100 mg of fish in a 1 ml aliquot of water and testing the supernatant, showing a higher tolerance of amplification inhibitors than a PCR assay. The LAMP assay did not generate a positive result when challenged with a range of non-target species, including Gadus macrocephalus, and Gadus chalcogrammus, indicating a high level of specificity. Direct detection of a positive reaction using propidium iodide was also demonstrated. Article in Journal/Newspaper atlantic cod Gadus morhua Liverpool John Moores University: LJMU Research Online Food Control 59 306 313 |
institution |
Open Polar |
collection |
Liverpool John Moores University: LJMU Research Online |
op_collection_id |
ftliverpooljmu |
language |
English |
topic |
TX341 Nutrition. Foods and food supply QH426 Genetics SH Aquaculture. Fisheries. Angling |
spellingShingle |
TX341 Nutrition. Foods and food supply QH426 Genetics SH Aquaculture. Fisheries. Angling Saull, J Duggan, C Hobbs, G Edwards, T The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
topic_facet |
TX341 Nutrition. Foods and food supply QH426 Genetics SH Aquaculture. Fisheries. Angling |
description |
Atlantic cod (Gadus morhua) is a commercially important species of white fish, and one of three species legally identifiable as cod in the UK. Mislabelling of G. morhua does occur, as does the substitution of G. morhua for less expensive species. Sensitive molecular tests based on PCR have been developed for this species, but they have limitations, including the need for expensive thermal cycling equipment, and complex DNA extraction procedures. A loop mediated isothermal amplification (LAMP) assay was designed for the G. morhua cytochrome b gene, which was capable of detecting 0.1% w/w G. morhua in a homogenised raw fish mix. The LAMP assay was also able to detect G. morhua DNA when a rapid sample preparation was used, involving heating 100 mg of fish in a 1 ml aliquot of water and testing the supernatant, showing a higher tolerance of amplification inhibitors than a PCR assay. The LAMP assay did not generate a positive result when challenged with a range of non-target species, including Gadus macrocephalus, and Gadus chalcogrammus, indicating a high level of specificity. Direct detection of a positive reaction using propidium iodide was also demonstrated. |
format |
Article in Journal/Newspaper |
author |
Saull, J Duggan, C Hobbs, G Edwards, T |
author_facet |
Saull, J Duggan, C Hobbs, G Edwards, T |
author_sort |
Saull, J |
title |
The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
title_short |
The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
title_full |
The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
title_fullStr |
The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
title_full_unstemmed |
The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process |
title_sort |
detection of atlantic cod (gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified dna extraction process |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://researchonline.ljmu.ac.uk/id/eprint/7559/ https://researchonline.ljmu.ac.uk/id/eprint/7559/3/The%20detection%20of%20Atlantic%20cod%20%28Gadus%20morhua%29%20using%20loop%20mediated%20isothermal%20amplification%20in%20conjunction%20with%20a%20simplified%20DNA%20extraction%20process.pdf https://doi.org/10.1016/j.foodcont.2015.05.038 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://researchonline.ljmu.ac.uk/id/eprint/7559/3/The%20detection%20of%20Atlantic%20cod%20%28Gadus%20morhua%29%20using%20loop%20mediated%20isothermal%20amplification%20in%20conjunction%20with%20a%20simplified%20DNA%20extraction%20process.pdf Saull, J, Duggan, C, Hobbs, G and Edwards, T (2015) The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process. Food Control, 59. pp. 306-313. ISSN 0956-7135 doi:10.1016/j.foodcont.2015.05.038 |
op_rights |
cc_by_nc_nd |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.foodcont.2015.05.038 |
container_title |
Food Control |
container_volume |
59 |
container_start_page |
306 |
op_container_end_page |
313 |
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1766357682820743168 |