Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges
Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high temporal and spatial precision. However, the use of historical eDNA...
Published in: | Molecular Ecology Resources |
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Online Access: | http://researchonline.ljmu.ac.uk/id/eprint/23933/ https://researchonline.ljmu.ac.uk/id/eprint/23933/1/Unlocking%20Antarctic%20molecular%20time%20capsules%20Recovering%20historical%20environmental%20DNA%20from%20museum%20preserved%20sponges.pdf https://doi.org/10.1111/1755-0998.14001 |
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ftliverpooljmu:oai:researchonline.ljmu.ac.uk:23933 2024-09-09T19:10:25+00:00 Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges Jeunen, GJ Mills, S Lamare, M Duffy, GA Knapp, M Stanton, JAL Mariani, S Treece, J Ferreira, S Durán-Vinet, B Zavodna, M Gemmell, NJ 2024-07-25 text http://researchonline.ljmu.ac.uk/id/eprint/23933/ https://researchonline.ljmu.ac.uk/id/eprint/23933/1/Unlocking%20Antarctic%20molecular%20time%20capsules%20Recovering%20historical%20environmental%20DNA%20from%20museum%20preserved%20sponges.pdf https://doi.org/10.1111/1755-0998.14001 en eng Wiley https://researchonline.ljmu.ac.uk/id/eprint/23933/1/Unlocking%20Antarctic%20molecular%20time%20capsules%20Recovering%20historical%20environmental%20DNA%20from%20museum%20preserved%20sponges.pdf Jeunen, GJ, Mills, S, Lamare, M, Duffy, GA, Knapp, M, Stanton, JAL, Mariani, S, Treece, J, Ferreira, S, Durán-Vinet, B, Zavodna, M and Gemmell, NJ (2024) Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges. Molecular Ecology Resources. e14001. ISSN 1755-098X doi:10.1111/1755-0998.14001 cc_by_nc_nd GE Environmental Sciences Article PeerReviewed 2024 ftliverpooljmu https://doi.org/10.1111/1755-0998.14001 2024-08-14T23:40:13Z Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high temporal and spatial precision. However, the use of historical eDNA, trapped within the vast number of specimens stored in scientific collections, opens up the opportunity to begin to reconstruct the communities and ecosystems of the past. Here, we define the term ‘heDNA’ to denote the historical environmental DNA that can be obtained from the recent past with high spatial and temporal accuracy. Using a variety of Antarctic sponge specimens stored in an extensive marine invertebrate collection, we were able to recover information on Antarctic fish biodiversity from specimens up to 20 years old. We successfully recovered 64 fish heDNA signals from 27 sponge specimens. Alpha diversity measures did not differ among preservation methods, but sponges stored frozen had a significantly different fish community composition compared to those stored dry or in ethanol. Our results show that we were consistently and reliably able to extract the heDNA trapped within marine sponge specimens, thereby enabling the reconstruction and investigation of communities and ecosystems of the recent past with a spatial and temporal resolution previously unattainable. Future research into heDNA extraction from other preservation methods, as well as the impact of specimen age and collection method, will strengthen and expand the opportunities for this novel resource to access new knowledge on ecological change during the last century. Article in Journal/Newspaper Antarc* Antarctic Liverpool John Moores University: LJMU Research Online Antarctic Molecular Ecology Resources 24 7 |
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Open Polar |
collection |
Liverpool John Moores University: LJMU Research Online |
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ftliverpooljmu |
language |
English |
topic |
GE Environmental Sciences |
spellingShingle |
GE Environmental Sciences Jeunen, GJ Mills, S Lamare, M Duffy, GA Knapp, M Stanton, JAL Mariani, S Treece, J Ferreira, S Durán-Vinet, B Zavodna, M Gemmell, NJ Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
topic_facet |
GE Environmental Sciences |
description |
Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high temporal and spatial precision. However, the use of historical eDNA, trapped within the vast number of specimens stored in scientific collections, opens up the opportunity to begin to reconstruct the communities and ecosystems of the past. Here, we define the term ‘heDNA’ to denote the historical environmental DNA that can be obtained from the recent past with high spatial and temporal accuracy. Using a variety of Antarctic sponge specimens stored in an extensive marine invertebrate collection, we were able to recover information on Antarctic fish biodiversity from specimens up to 20 years old. We successfully recovered 64 fish heDNA signals from 27 sponge specimens. Alpha diversity measures did not differ among preservation methods, but sponges stored frozen had a significantly different fish community composition compared to those stored dry or in ethanol. Our results show that we were consistently and reliably able to extract the heDNA trapped within marine sponge specimens, thereby enabling the reconstruction and investigation of communities and ecosystems of the recent past with a spatial and temporal resolution previously unattainable. Future research into heDNA extraction from other preservation methods, as well as the impact of specimen age and collection method, will strengthen and expand the opportunities for this novel resource to access new knowledge on ecological change during the last century. |
format |
Article in Journal/Newspaper |
author |
Jeunen, GJ Mills, S Lamare, M Duffy, GA Knapp, M Stanton, JAL Mariani, S Treece, J Ferreira, S Durán-Vinet, B Zavodna, M Gemmell, NJ |
author_facet |
Jeunen, GJ Mills, S Lamare, M Duffy, GA Knapp, M Stanton, JAL Mariani, S Treece, J Ferreira, S Durán-Vinet, B Zavodna, M Gemmell, NJ |
author_sort |
Jeunen, GJ |
title |
Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
title_short |
Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
title_full |
Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
title_fullStr |
Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
title_full_unstemmed |
Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges |
title_sort |
unlocking antarctic molecular time-capsules – recovering historical environmental dna from museum-preserved sponges |
publisher |
Wiley |
publishDate |
2024 |
url |
http://researchonline.ljmu.ac.uk/id/eprint/23933/ https://researchonline.ljmu.ac.uk/id/eprint/23933/1/Unlocking%20Antarctic%20molecular%20time%20capsules%20Recovering%20historical%20environmental%20DNA%20from%20museum%20preserved%20sponges.pdf https://doi.org/10.1111/1755-0998.14001 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://researchonline.ljmu.ac.uk/id/eprint/23933/1/Unlocking%20Antarctic%20molecular%20time%20capsules%20Recovering%20historical%20environmental%20DNA%20from%20museum%20preserved%20sponges.pdf Jeunen, GJ, Mills, S, Lamare, M, Duffy, GA, Knapp, M, Stanton, JAL, Mariani, S, Treece, J, Ferreira, S, Durán-Vinet, B, Zavodna, M and Gemmell, NJ (2024) Unlocking Antarctic molecular time-capsules – Recovering historical environmental DNA from museum-preserved sponges. Molecular Ecology Resources. e14001. ISSN 1755-098X doi:10.1111/1755-0998.14001 |
op_rights |
cc_by_nc_nd |
op_doi |
https://doi.org/10.1111/1755-0998.14001 |
container_title |
Molecular Ecology Resources |
container_volume |
24 |
container_issue |
7 |
_version_ |
1809825472923566080 |