Anguilla anguilla ir A. japonica individų rūšinės priklausomybės nustatymas molekuliniu metodu, pagrįstu mtDNR D-kilpos regiono homologinių sekų analize

• Main Authors: Ragauskas, Adomas, Butkauskas, Dalius, Sruoga, Aniolas
• Format: Article in Journal/Newspaper
• Language: Lithuanian; English
• Published: 2011
• Subjects: Anguilla sp.; Molecular technique; D-loop; Species identification; info:eu-repo/classification/udc/57; Anguilla anguilla
• Online Access: http://lmavb.lvb.lt/LMAVB:ELABAPDB5924089&prefLang=en_US

In order to clarify whether declared eels belong to A. japonica species or not, molecular technique which is based on PCR with Ang1 primer pair and alignment of newly obtained sequences with homologous A. anguilla, A. rostrata and A. japonica sequences from the GenBank, was used. It was shown that identification of A. anguilla, A. rostrata and A. japonica species based on analysis of 450-455 bp homologous mtDNA D-loop region sequences is reliable. After the study of 31 eels of uncertain origin it became clear that all investigated mtDNA D-loop region sequences belong to A. japonica species. Since this molecular technique is a powerful tool for A. anguilla and A. japonica discrimination, thus it could be used as an alternative to other methods.