Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation
Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. There...
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ftlitinstagrecon:oai:elaba:5864410 2023-05-15T17:10:55+02:00 Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas 2008 http://lmavb.lvb.lt/LMAVB:ELABAPDB5864410&prefLang=en_US eng eng http://lmavb.lvb.lt/LMAVB:ELABAPDB5864410&prefLang=en_US Proceedings of the Latvian Academy of Sciences. Section B, 2008, Vol. 62, no. 6, p. 215-218 ISSN 1407-009X Anseriformes Sea ducks Microsatellites Genetic variability info:eu-repo/semantics/article 2008 ftlitinstagrecon 2021-12-02T00:37:18Z Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. Therefore, the objective of our work was to examine whether specific primers used previously for Anatidae, Phasianidae and Laridae taxons could amplify microsatellite loci of sea duck species: Long-tailed duck (Clangula hyemalis), Goosander (Mergus merganser) and Velvet Scoter (Melanitta fusca). Tissue samples were collected and DNA was extracted by rapid salt extraction method. Amplification of DNA fragments was carried out using specific microsatellite primers of APH21, Aalmu1, Sfimu4, Sfimu5 (Anatidae), ADL209, ADL115 (Phasianidae) and K71, RGB28 (Laridae). Four primer pairs (APH21, Aalmu1, K71, and nSfimu4) were suitable for investigation of interspecies genetic variability among Long-tailed duck and Velvet Scoter. Intraspecies specificity has been detected for primer pair ADL 209 in all three duck species. The primer pair APH21 was selected as most promising for investigation of intraspecies variability of Long-tailed duck and Velvet Scoter. Article in Journal/Newspaper Melanitta fusca velvet scoter LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
institution |
Open Polar |
collection |
LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
English |
topic |
Anseriformes Sea ducks Microsatellites Genetic variability |
spellingShingle |
Anseriformes Sea ducks Microsatellites Genetic variability Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
topic_facet |
Anseriformes Sea ducks Microsatellites Genetic variability |
description |
Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. Therefore, the objective of our work was to examine whether specific primers used previously for Anatidae, Phasianidae and Laridae taxons could amplify microsatellite loci of sea duck species: Long-tailed duck (Clangula hyemalis), Goosander (Mergus merganser) and Velvet Scoter (Melanitta fusca). Tissue samples were collected and DNA was extracted by rapid salt extraction method. Amplification of DNA fragments was carried out using specific microsatellite primers of APH21, Aalmu1, Sfimu4, Sfimu5 (Anatidae), ADL209, ADL115 (Phasianidae) and K71, RGB28 (Laridae). Four primer pairs (APH21, Aalmu1, K71, and nSfimu4) were suitable for investigation of interspecies genetic variability among Long-tailed duck and Velvet Scoter. Intraspecies specificity has been detected for primer pair ADL 209 in all three duck species. The primer pair APH21 was selected as most promising for investigation of intraspecies variability of Long-tailed duck and Velvet Scoter. |
format |
Article in Journal/Newspaper |
author |
Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas |
author_facet |
Sruoga, Aniolas Slavėnaitė, Sigita Butkauskas, Dalius Gražulevičius, Gediminas |
author_sort |
Sruoga, Aniolas |
title |
Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
title_short |
Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
title_full |
Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
title_fullStr |
Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
title_full_unstemmed |
Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation |
title_sort |
cross-species applicability of microsatellite markers for investigation of sea ducks (mergini) genetic differentiation |
publishDate |
2008 |
url |
http://lmavb.lvb.lt/LMAVB:ELABAPDB5864410&prefLang=en_US |
genre |
Melanitta fusca velvet scoter |
genre_facet |
Melanitta fusca velvet scoter |
op_source |
Proceedings of the Latvian Academy of Sciences. Section B, 2008, Vol. 62, no. 6, p. 215-218 ISSN 1407-009X |
op_relation |
http://lmavb.lvb.lt/LMAVB:ELABAPDB5864410&prefLang=en_US |
_version_ |
1766067581198794752 |