Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus
Lichen is a symbiotic association composed of photosynthetic partner such as green alga or cyanobacterium, and a fungal partner (Nash, 1996). Cetraria islandica (L.) Ach. (Iceland moss) is a foliaceous lichen growing mostly in central Europe, North America and Siberia (Evans, 1994). Iceland moss hel...
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ftlitinstagrecon:oai:elaba:5707791 2023-05-15T16:48:43+02:00 Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus Juodsnukytė, Jovita Savickienė, Nijolė Savickas, Arūnas Žvirblytė, Gitana Kandelinskaya, Olga Grischenko, Helena 2014 http://lsmu.lvb.lt/LSMU:ELABAPDB5707791&prefLang=en_US eng eng http://lsmu.lvb.lt/LSMU:ELABAPDB5707791&prefLang=en_US The 5th International Conference On Pharmaceutical Sciences and Pharmacy Practice dedicated to 145th Anniversary of prof. Petras Raudonikis : November 22, 2014 Kaunas, Lithuania : Book of abstracts / Faculty of Pharmacy of Lithuanian university of Health Sciences. LSMU FF Alumni Association. Lithuanian Pharmaceutical Association. Lithuanian Pharmacists’ Society Edited by: Indrė Šveikauskaitė, Kaunas : Lietuvos sveikatos mokslų universitetas, 2014, p. 41-43 : ISBN 9789955153726 Lichens Chemistry Plant proteins Analysis Chromatography gel Methods info:eu-repo/semantics/conferencePaper 2014 ftlitinstagrecon 2021-12-02T00:28:40Z Lichen is a symbiotic association composed of photosynthetic partner such as green alga or cyanobacterium, and a fungal partner (Nash, 1996). Cetraria islandica (L.) Ach. (Iceland moss) is a foliaceous lichen growing mostly in central Europe, North America and Siberia (Evans, 1994). Iceland moss helps to relief dry cough, improves digestion and has antiemetic as well as immunostimulatory and anti-inflammatory effects. Cetraria islandica (L.) Ach. is a plant rich of polysaccharides, lichen acids (Ingolfsdottir et al., 1998). Cetraria islandica (L.) Ach. is also enriched with polypeptides, lichen acids and water-soluble carbohydrates. The most important of them is lichenan, (1→3,1→4) β – D – glucan (Puodžiūnienė el al., 2005; Abbott and Boraston, 2009). Proteins binding lichenan and izolichenan can be determined using Bradford dyeing method (Freysdotirr et al. 2008). For the determination of other proteins are often used Bradford and SDSPAGE assays (Vergara-Barberán et al., 2014). Aim of experiment: To determine the quantity and molecular mass of proteins isolated from Cetraria islandica (L.) Ach. thallus. Experiment tasks: 1. To separate proteins into fraction, using Size Exclusion Chromatography; 2. To estimate the quantity and molecular mass of proteins. Materials and methods: 1. Protein fractions isolated from Cetraria islandica (L.) Ach. thallus were achieved from V.F.Kuprevich Institute of Experimental Botany of Belarus Academy of Sciences. 2. Size Exclusion Chromatography was used for the proteinseparation into fractions. Size Exclusion Chromatography was performed with AKTA Purifier system, using Superdex 200 10/300 GL column. 3. The quantity of protein was measured by Bradford method using bovine serum albumin BSA (Sigma –Aldrich, USA) protein standards. 4. The molecular mass of proteins was estimated by using of Sodium dodecyl sulfate polyakrylamide gel electrophoresis SDS – PAGE. 5. Results were analyzed with MS Excel. [.]. Conference Object Iceland Siberia LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Nash ENVELOPE(-62.350,-62.350,-74.233,-74.233) Abbott ENVELOPE(-62.133,-62.133,-64.100,-64.100) Aldrich ENVELOPE(158.217,158.217,-80.117,-80.117) |
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Open Polar |
collection |
LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
English |
topic |
Lichens Chemistry Plant proteins Analysis Chromatography gel Methods |
spellingShingle |
Lichens Chemistry Plant proteins Analysis Chromatography gel Methods Juodsnukytė, Jovita Savickienė, Nijolė Savickas, Arūnas Žvirblytė, Gitana Kandelinskaya, Olga Grischenko, Helena Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
topic_facet |
Lichens Chemistry Plant proteins Analysis Chromatography gel Methods |
description |
Lichen is a symbiotic association composed of photosynthetic partner such as green alga or cyanobacterium, and a fungal partner (Nash, 1996). Cetraria islandica (L.) Ach. (Iceland moss) is a foliaceous lichen growing mostly in central Europe, North America and Siberia (Evans, 1994). Iceland moss helps to relief dry cough, improves digestion and has antiemetic as well as immunostimulatory and anti-inflammatory effects. Cetraria islandica (L.) Ach. is a plant rich of polysaccharides, lichen acids (Ingolfsdottir et al., 1998). Cetraria islandica (L.) Ach. is also enriched with polypeptides, lichen acids and water-soluble carbohydrates. The most important of them is lichenan, (1→3,1→4) β – D – glucan (Puodžiūnienė el al., 2005; Abbott and Boraston, 2009). Proteins binding lichenan and izolichenan can be determined using Bradford dyeing method (Freysdotirr et al. 2008). For the determination of other proteins are often used Bradford and SDSPAGE assays (Vergara-Barberán et al., 2014). Aim of experiment: To determine the quantity and molecular mass of proteins isolated from Cetraria islandica (L.) Ach. thallus. Experiment tasks: 1. To separate proteins into fraction, using Size Exclusion Chromatography; 2. To estimate the quantity and molecular mass of proteins. Materials and methods: 1. Protein fractions isolated from Cetraria islandica (L.) Ach. thallus were achieved from V.F.Kuprevich Institute of Experimental Botany of Belarus Academy of Sciences. 2. Size Exclusion Chromatography was used for the proteinseparation into fractions. Size Exclusion Chromatography was performed with AKTA Purifier system, using Superdex 200 10/300 GL column. 3. The quantity of protein was measured by Bradford method using bovine serum albumin BSA (Sigma –Aldrich, USA) protein standards. 4. The molecular mass of proteins was estimated by using of Sodium dodecyl sulfate polyakrylamide gel electrophoresis SDS – PAGE. 5. Results were analyzed with MS Excel. [.]. |
format |
Conference Object |
author |
Juodsnukytė, Jovita Savickienė, Nijolė Savickas, Arūnas Žvirblytė, Gitana Kandelinskaya, Olga Grischenko, Helena |
author_facet |
Juodsnukytė, Jovita Savickienė, Nijolė Savickas, Arūnas Žvirblytė, Gitana Kandelinskaya, Olga Grischenko, Helena |
author_sort |
Juodsnukytė, Jovita |
title |
Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
title_short |
Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
title_full |
Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
title_fullStr |
Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
title_full_unstemmed |
Fractionation and determination of proteins isolated from Cetraria islandica (L.) Ach. thallus |
title_sort |
fractionation and determination of proteins isolated from cetraria islandica (l.) ach. thallus |
publishDate |
2014 |
url |
http://lsmu.lvb.lt/LSMU:ELABAPDB5707791&prefLang=en_US |
long_lat |
ENVELOPE(-62.350,-62.350,-74.233,-74.233) ENVELOPE(-62.133,-62.133,-64.100,-64.100) ENVELOPE(158.217,158.217,-80.117,-80.117) |
geographic |
Nash Abbott Aldrich |
geographic_facet |
Nash Abbott Aldrich |
genre |
Iceland Siberia |
genre_facet |
Iceland Siberia |
op_source |
The 5th International Conference On Pharmaceutical Sciences and Pharmacy Practice dedicated to 145th Anniversary of prof. Petras Raudonikis : November 22, 2014 Kaunas, Lithuania : Book of abstracts / Faculty of Pharmacy of Lithuanian university of Health Sciences. LSMU FF Alumni Association. Lithuanian Pharmaceutical Association. Lithuanian Pharmacists’ Society Edited by: Indrė Šveikauskaitė, Kaunas : Lietuvos sveikatos mokslų universitetas, 2014, p. 41-43 : ISBN 9789955153726 |
op_relation |
http://lsmu.lvb.lt/LSMU:ELABAPDB5707791&prefLang=en_US |
_version_ |
1766038796918325248 |