Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence
Health impact of metal mixture at environment realistic concentrations are difficult to predict especially for long-term effects where cause-and-effect relationships may not be directly obvious. This study was aimed to evaluate metal mixture (Zn—0.1, Cu—0.01, Ni—0.01, Cr—0.01, Pb—0.005 and Cd—0.005...
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ftlitinstagrecon:oai:elaba:56018585 2023-05-15T15:31:54+02:00 Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence Stankevičiūtė, Milda Sauliutė, Gintarė Svecevičius, Gintaras Kazlauskienė, Nijolė Baršienė, Janina 2017 http://gtc.lvb.lt/GTC:ELABAPDB56018585&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10646-017-1833-0 http://gtc.lvb.lt/GTC:ELABAPDB56018585&prefLang=en_US Ecotoxicology, Dordrecht : Springer New York LLC, 2017, vol. 26, iss. 8, p. 1051-1064 ISSN 0963-9292 eISSN 1573-3017 Bioaccumulation Cytotoxicity Genotoxicity Metal mixture Salmo salar Steady-state info:eu-repo/semantics/article 2017 ftlitinstagrecon https://doi.org/10.1007/s10646-017-1833-0 2021-12-02T00:45:15Z Health impact of metal mixture at environment realistic concentrations are difficult to predict especially for long-term effects where cause-and-effect relationships may not be directly obvious. This study was aimed to evaluate metal mixture (Zn—0.1, Cu—0.01, Ni—0.01, Cr—0.01, Pb—0.005 and Cd—0.005 mg/L, respectively for 1, 2, 4, 7, 14 and 28 days at concentrations accepted for the inland waters in EU) genotoxicity (micronuclei, nuclear buds, nuclear buds on filament), cytotoxicity (8-shaped nuclei, fragmented-apoptotic erythrocytes), bioaccumulation, steady-state and the reference level of geno-cytotoxicity in hatchery-reared Atlantic salmon tissues. Metals accumulated mostly in gills and kidneys, to the lesser extent in the muscle. Uptake of metals from an entire mixture in the fish for 14 days is sufficient to reach steady-state Cr, Pb concentrations in all tissues; Zn, Cu—in kidneys and muscle, Ni—in liver, kidneys, muscle and Cd—in muscle. Treatment with metal mixture significantly increased summed genotoxicity levels at 7 days of exposure in peripheral blood and liver erythrocytes, at 14 days of exposure in gills and kidney erythrocytes. Significant elevation of cytotoxicity was detected after 2 and 14 days of exposure in gills erythrocytes and after 28 days—in peripheral blood erythrocytes. The amount of Cu, Cr, Pb and Cd accumulated in tissues was dependent upon duration of exposure; nuclear buds, 8-shaped nuclei frequencies also were dependent upon duration of exposure. This study indicates that metals at low levels when existing in mixture causes significant geno-cytotoxicity responses and metals bioaccumulation in salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Ecotoxicology 26 8 1051 1064 |
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Open Polar |
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LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
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ftlitinstagrecon |
language |
English |
topic |
Bioaccumulation Cytotoxicity Genotoxicity Metal mixture Salmo salar Steady-state |
spellingShingle |
Bioaccumulation Cytotoxicity Genotoxicity Metal mixture Salmo salar Steady-state Stankevičiūtė, Milda Sauliutė, Gintarė Svecevičius, Gintaras Kazlauskienė, Nijolė Baršienė, Janina Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
topic_facet |
Bioaccumulation Cytotoxicity Genotoxicity Metal mixture Salmo salar Steady-state |
description |
Health impact of metal mixture at environment realistic concentrations are difficult to predict especially for long-term effects where cause-and-effect relationships may not be directly obvious. This study was aimed to evaluate metal mixture (Zn—0.1, Cu—0.01, Ni—0.01, Cr—0.01, Pb—0.005 and Cd—0.005 mg/L, respectively for 1, 2, 4, 7, 14 and 28 days at concentrations accepted for the inland waters in EU) genotoxicity (micronuclei, nuclear buds, nuclear buds on filament), cytotoxicity (8-shaped nuclei, fragmented-apoptotic erythrocytes), bioaccumulation, steady-state and the reference level of geno-cytotoxicity in hatchery-reared Atlantic salmon tissues. Metals accumulated mostly in gills and kidneys, to the lesser extent in the muscle. Uptake of metals from an entire mixture in the fish for 14 days is sufficient to reach steady-state Cr, Pb concentrations in all tissues; Zn, Cu—in kidneys and muscle, Ni—in liver, kidneys, muscle and Cd—in muscle. Treatment with metal mixture significantly increased summed genotoxicity levels at 7 days of exposure in peripheral blood and liver erythrocytes, at 14 days of exposure in gills and kidney erythrocytes. Significant elevation of cytotoxicity was detected after 2 and 14 days of exposure in gills erythrocytes and after 28 days—in peripheral blood erythrocytes. The amount of Cu, Cr, Pb and Cd accumulated in tissues was dependent upon duration of exposure; nuclear buds, 8-shaped nuclei frequencies also were dependent upon duration of exposure. This study indicates that metals at low levels when existing in mixture causes significant geno-cytotoxicity responses and metals bioaccumulation in salmon. |
format |
Article in Journal/Newspaper |
author |
Stankevičiūtė, Milda Sauliutė, Gintarė Svecevičius, Gintaras Kazlauskienė, Nijolė Baršienė, Janina |
author_facet |
Stankevičiūtė, Milda Sauliutė, Gintarė Svecevičius, Gintaras Kazlauskienė, Nijolė Baršienė, Janina |
author_sort |
Stankevičiūtė, Milda |
title |
Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
title_short |
Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
title_full |
Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
title_fullStr |
Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
title_full_unstemmed |
Genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (Zn, Cu, Ni, Cr, Pb, Cd) accumulated in Atlantic salmon (Salmo salar). Part I: importance of exposure time and tissue dependence |
title_sort |
genotoxicity and cytotoxicity response to environmentally relevant complex metal mixture (zn, cu, ni, cr, pb, cd) accumulated in atlantic salmon (salmo salar). part i: importance of exposure time and tissue dependence |
publishDate |
2017 |
url |
http://gtc.lvb.lt/GTC:ELABAPDB56018585&prefLang=en_US |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Ecotoxicology, Dordrecht : Springer New York LLC, 2017, vol. 26, iss. 8, p. 1051-1064 ISSN 0963-9292 eISSN 1573-3017 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10646-017-1833-0 http://gtc.lvb.lt/GTC:ELABAPDB56018585&prefLang=en_US |
op_doi |
https://doi.org/10.1007/s10646-017-1833-0 |
container_title |
Ecotoxicology |
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26 |
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8 |
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1051 |
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1064 |
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1766362403093610496 |