Induction of micronuclei in Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus) after treatment with bisphenol A, diallyl phthalate and tetrabromodiphenyl ether-47

• Main Authors: Baršienė, Janina, Dedonytė, Veronika, Rybakovas, Aleksandras, Andreikėnaitė, Laura, Andersen, Odd-Ketil
• Format: Article in Journal/Newspaper
• Language: Lithuanian; English
• Published: 2005
• Subjects: Micronuclei; Cod; Turbot; Bisphenol A; Diallyl phthalate; atlantic cod; Gadus morhua; Scophthalmus maximus
• Online Access: http://lmavb.lvb.lt/LMAVB:ELABAPDB5554691&prefLang=en_US

An in vivo study on the genotoxic effects of endocrine disruptors and flame retardant was carried out with marine fish Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus). The fish were exposed for 3 weeks to a sublethal concentration of bisphenol A, diallyl phthalate (50 ppb concentration for both) and to tetrabromodiphenyl ether BDE-47 (nominal concentration 5 ppb). Micronuclei (MN) were analyzed in blood (mature erythrocytes) and in immature erythrocytes of cephalic kidney. The mean MN frequencies in the cod ranged from 0.17 to 1.37 MN/1000 erythrocytes and in turbot from 0.60 to 1.18 MN/1000 erythrocytes. A significant increase of micronuclei was observed in turbot blood (P < 0.0001; Mann-Whitney U-test) and cod cephalic kidney after treatment with all three compounds (P values ranged from 0.0401 to 0.0027). In turbot blood, MN incidence increased 6.7-fold after exposure to bisphenol A, 9.4-fold after exposure to diallyl phthalate, and an 11.8-fold elevation was detected after treatment with tetrabromodiphenyl ether BDE-47. Treatment with either of the three compounds did not elevate MN formation in the blood of Atlantic cod and in the cephalic kidney of turbot. A species-specific response to bisphenol A and diallyl phthalate was observed in both types of erythrocytes, and tissue-specific differences in MN induction were found in both species after exposure to BDE-47.