Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia
Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocyst...
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ftlitinstagrecon:oai:elaba:53519997 2023-05-15T18:05:02+02:00 Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia Prakas, Petras Kirillova, Viktorija Gavarāne, Inese Grāvele, Evita Butkauskas, Dalius Rudaitytė-Lukošienė, Eglė Kirjušina, Muza 2019 http://gtc.lvb.lt/GTC:ELABAPDB53519997&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-019-06393-9 http://gtc.lvb.lt/GTC:ELABAPDB53519997&prefLang=en_US Parasitology research, New York : Springer, 2019, vol. 118, iss. 9, p. 2689-2694 ISSN 0932-0113 eISSN 1432-1955 Black rat Sarcocystis ratti Transmission electron microscopy rRNA cox1 Phylogeny info:eu-repo/semantics/article 2019 ftlitinstagrecon https://doi.org/10.1007/s00436-019-06393-9 2021-12-02T00:43:19Z Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. sp. Under a light microscope, sarcocysts were ribbon-shaped, 0.9-1.3 x 0.09-0.14 mm in size and had a thin (0.8-1.3 mu m) and smooth cyst wall. The lancet-shaped bradyzoites were 8.3 x 4.3 (7.5-9.3 x 3.9-4.8) mu m. Under a transmission electron microscope, the cyst wall was up to 1.3 mu m thick, wavy, the ground substance appeared smooth, type 1a-like. Morphologically, sarcocysts of S. ratti were somewhat similar to those of S. cymruensis, S. rodentifelis, and S. dispersa-like previously identified in the brown rat (Rattus norvegicus). On the basis of 18S rDNA, 28S rDNA, and cox1, significant genetic differences (at least 2.3, 4.5, and 5.8%, respectively) were observed when comparing S. ratti with other Sarcocystis species using rodents as intermediate hosts. While ITS1 sequences of S. ratti were highly distinct from other Sarcocystis species available in GenBank. Phylogenetic and ecological data suggest that predatory mammals living near households are definitive hosts of S. ratti. Article in Journal/Newspaper Rattus rattus LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Parasitology Research 118 9 2689 2694 |
institution |
Open Polar |
collection |
LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
English |
topic |
Black rat Sarcocystis ratti Transmission electron microscopy rRNA cox1 Phylogeny |
spellingShingle |
Black rat Sarcocystis ratti Transmission electron microscopy rRNA cox1 Phylogeny Prakas, Petras Kirillova, Viktorija Gavarāne, Inese Grāvele, Evita Butkauskas, Dalius Rudaitytė-Lukošienė, Eglė Kirjušina, Muza Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
topic_facet |
Black rat Sarcocystis ratti Transmission electron microscopy rRNA cox1 Phylogeny |
description |
Rodents have been widely studied as intermediate hosts of Sarcocystis; however, only a few reports on these parasites in the black rat (Rattus rattus) are known. Having examined 13 black rats captured in Latvia, sarcocysts were found in skeletal muscles of two mammals and were described as Sarcocystis ratti n. sp. Under a light microscope, sarcocysts were ribbon-shaped, 0.9-1.3 x 0.09-0.14 mm in size and had a thin (0.8-1.3 mu m) and smooth cyst wall. The lancet-shaped bradyzoites were 8.3 x 4.3 (7.5-9.3 x 3.9-4.8) mu m. Under a transmission electron microscope, the cyst wall was up to 1.3 mu m thick, wavy, the ground substance appeared smooth, type 1a-like. Morphologically, sarcocysts of S. ratti were somewhat similar to those of S. cymruensis, S. rodentifelis, and S. dispersa-like previously identified in the brown rat (Rattus norvegicus). On the basis of 18S rDNA, 28S rDNA, and cox1, significant genetic differences (at least 2.3, 4.5, and 5.8%, respectively) were observed when comparing S. ratti with other Sarcocystis species using rodents as intermediate hosts. While ITS1 sequences of S. ratti were highly distinct from other Sarcocystis species available in GenBank. Phylogenetic and ecological data suggest that predatory mammals living near households are definitive hosts of S. ratti. |
format |
Article in Journal/Newspaper |
author |
Prakas, Petras Kirillova, Viktorija Gavarāne, Inese Grāvele, Evita Butkauskas, Dalius Rudaitytė-Lukošienė, Eglė Kirjušina, Muza |
author_facet |
Prakas, Petras Kirillova, Viktorija Gavarāne, Inese Grāvele, Evita Butkauskas, Dalius Rudaitytė-Lukošienė, Eglė Kirjušina, Muza |
author_sort |
Prakas, Petras |
title |
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
title_short |
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
title_full |
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
title_fullStr |
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
title_full_unstemmed |
Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia |
title_sort |
morphological and molecular description of sarcocystis ratti n. sp. from the black rat (rattus rattus) in latvia |
publishDate |
2019 |
url |
http://gtc.lvb.lt/GTC:ELABAPDB53519997&prefLang=en_US |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Parasitology research, New York : Springer, 2019, vol. 118, iss. 9, p. 2689-2694 ISSN 0932-0113 eISSN 1432-1955 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-019-06393-9 http://gtc.lvb.lt/GTC:ELABAPDB53519997&prefLang=en_US |
op_doi |
https://doi.org/10.1007/s00436-019-06393-9 |
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Parasitology Research |
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118 |
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9 |
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2689 |
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2694 |
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1766176460133892096 |