The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation
The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where...
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ftlitinstagrecon:oai:elaba:4673214 2023-05-15T17:23:49+02:00 The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation Kisnierienė, Vilma Ditchenko, Tatiana I. Kudryashov, Anatoly P. Sakalauskas, Vidmantas Yurin, Vladimir M. Rukšėnas, Osvaldas 2012 http://vu.lvb.lt/VU:ELABAPDB4673214&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.2478/s11535-012-0085-5 http://vu.lvb.lt/VU:ELABAPDB4673214&prefLang=en_US Central European Journal of Biology, Warsaw : Versita, 2012, Vol. 7, no. 6, p. 1066-1075 ISSN 1895-104X Characeae Potassium channels Acetylcholine Plant electrical signaling Action potential info:eu-repo/semantics/article 2012 ftlitinstagrecon https://doi.org/10.2478/s11535-012-0085-5 2021-12-02T01:17:07Z The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants. Article in Journal/Newspaper Nitella flexilis LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Open Life Sciences 7 6 1066 1075 |
institution |
Open Polar |
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LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
English |
topic |
Characeae Potassium channels Acetylcholine Plant electrical signaling Action potential |
spellingShingle |
Characeae Potassium channels Acetylcholine Plant electrical signaling Action potential Kisnierienė, Vilma Ditchenko, Tatiana I. Kudryashov, Anatoly P. Sakalauskas, Vidmantas Yurin, Vladimir M. Rukšėnas, Osvaldas The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
topic_facet |
Characeae Potassium channels Acetylcholine Plant electrical signaling Action potential |
description |
The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants. |
format |
Article in Journal/Newspaper |
author |
Kisnierienė, Vilma Ditchenko, Tatiana I. Kudryashov, Anatoly P. Sakalauskas, Vidmantas Yurin, Vladimir M. Rukšėnas, Osvaldas |
author_facet |
Kisnierienė, Vilma Ditchenko, Tatiana I. Kudryashov, Anatoly P. Sakalauskas, Vidmantas Yurin, Vladimir M. Rukšėnas, Osvaldas |
author_sort |
Kisnierienė, Vilma |
title |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_short |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_full |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_fullStr |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_full_unstemmed |
The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation |
title_sort |
effect of acetylcholine on characeae k+ channels at rest and during action potential generation |
publishDate |
2012 |
url |
http://vu.lvb.lt/VU:ELABAPDB4673214&prefLang=en_US |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_source |
Central European Journal of Biology, Warsaw : Versita, 2012, Vol. 7, no. 6, p. 1066-1075 ISSN 1895-104X |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.2478/s11535-012-0085-5 http://vu.lvb.lt/VU:ELABAPDB4673214&prefLang=en_US |
op_doi |
https://doi.org/10.2478/s11535-012-0085-5 |
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Open Life Sciences |
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7 |
container_issue |
6 |
container_start_page |
1066 |
op_container_end_page |
1075 |
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1766114280881520640 |