Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia,...
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ftlitinstagrecon:oai:elaba:33583292 2023-05-15T18:05:30+02:00 Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania Simanavičius, Martynas Juškaitė, Karolina Verbickaitė, Arūnė Jasiulionis, Marius Tamošiūnas, Paulius Lukas Petraitytė-Burneikienė, Rasa Žvirblienė, Aurelija Ulrich, Rainer G. Kučinskaitė-Kodzė, Indrė 2018 http://vu.lvb.lt/VU:ELABAPDB33583292&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2018.06.014 http://vu.lvb.lt/VU:ELABAPDB33583292&prefLang=en_US Veterinary microbiology, Amsterdam : Elsevier, 2018, vol. 221, p. 129-133 ISSN 0378-1135 Hepatitis E virus (HEV) Rat HEV Genotype C1 info:eu-repo/semantics/article 2018 ftlitinstagrecon https://doi.org/10.1016/j.vetmic.2018.06.014 2021-12-02T00:10:57Z Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1-4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1-4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non persistent nature of HEV infection. Article in Journal/Newspaper Rattus rattus LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Norway Veterinary Microbiology 221 129 133 |
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Open Polar |
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LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
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ftlitinstagrecon |
language |
English |
topic |
Hepatitis E virus (HEV) Rat HEV Genotype C1 |
spellingShingle |
Hepatitis E virus (HEV) Rat HEV Genotype C1 Simanavičius, Martynas Juškaitė, Karolina Verbickaitė, Arūnė Jasiulionis, Marius Tamošiūnas, Paulius Lukas Petraitytė-Burneikienė, Rasa Žvirblienė, Aurelija Ulrich, Rainer G. Kučinskaitė-Kodzė, Indrė Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
topic_facet |
Hepatitis E virus (HEV) Rat HEV Genotype C1 |
description |
Rat hepatitis E virus (HEV) is an orthohepevirus which is related to other HEV found in humans and other mammals. It was first identified in Norway rats (Rattus norvegicus) from Germany in 2010, and later it has been detected in Black rats (Rattus rattus) and Norway rats from USA, China, Indonesia, Vietnam and many European countries. In this study, we describe molecular and serological investigations of Black and Norway rats trapped in Lithuania, Eastern Europe, for infections with rat HEV and human HEV genotypes 1-4. Rat HEV-specific real-time reverse transcription-PCR (RT-qPCR) analysis of rat liver samples revealed the presence of rat HEV in 9 of 109 (8.3%) samples. In contrast, a RT-qPCR specific for HEV genotypes 1-4 did not reveal any positive samples. A nested broad spectrum RT-PCR was used for a confirmation of rat HEV infection with a subsequent sequencing of the amplified rat HEV genome fragment. Phylogenetic analysis revealed a clustering of all newly identified rat HEV sequences with Norway rat-derived rat HEV sequences from Germany within the species Orthohepevirus C. An indirect ELISA using a yeast-expressed truncated rat HEV capsid protein variant revealed 31.2% seropositive samples indicating a high rate of rat HEV circulation in the rat population examined. In conclusion, the current investigation confirms rat HEV infections in Norway and Black rats in Lithuania, Eastern Europe, and the non persistent nature of HEV infection. |
format |
Article in Journal/Newspaper |
author |
Simanavičius, Martynas Juškaitė, Karolina Verbickaitė, Arūnė Jasiulionis, Marius Tamošiūnas, Paulius Lukas Petraitytė-Burneikienė, Rasa Žvirblienė, Aurelija Ulrich, Rainer G. Kučinskaitė-Kodzė, Indrė |
author_facet |
Simanavičius, Martynas Juškaitė, Karolina Verbickaitė, Arūnė Jasiulionis, Marius Tamošiūnas, Paulius Lukas Petraitytė-Burneikienė, Rasa Žvirblienė, Aurelija Ulrich, Rainer G. Kučinskaitė-Kodzė, Indrė |
author_sort |
Simanavičius, Martynas |
title |
Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
title_short |
Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
title_full |
Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
title_fullStr |
Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
title_full_unstemmed |
Detection of rat hepatitis E virus, but not human pathogenic hepatitis E virus genotype 1-4 infections in wild rats from Lithuania |
title_sort |
detection of rat hepatitis e virus, but not human pathogenic hepatitis e virus genotype 1-4 infections in wild rats from lithuania |
publishDate |
2018 |
url |
http://vu.lvb.lt/VU:ELABAPDB33583292&prefLang=en_US |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Veterinary microbiology, Amsterdam : Elsevier, 2018, vol. 221, p. 129-133 ISSN 0378-1135 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2018.06.014 http://vu.lvb.lt/VU:ELABAPDB33583292&prefLang=en_US |
op_doi |
https://doi.org/10.1016/j.vetmic.2018.06.014 |
container_title |
Veterinary Microbiology |
container_volume |
221 |
container_start_page |
129 |
op_container_end_page |
133 |
_version_ |
1766176969733439488 |