A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas
In a world of declining biodiversity, monitoring is becoming crucial. Molecular methods, such as metabarcoding, have the potential to rapidly expand our knowledge of biodiversity, supporting assessment, management, and conservation. In the marine environment, where hard substrata are more difficult...
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ftlitinstagrecon:oai:elaba:31202685 2023-05-15T17:41:36+02:00 A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas Cahill, Abigail E. Pearman, John K. Borja, Angel Carugati, Laura Carvalho, Susana Danovaro, Roberto Dashfield, Sarah David, Romain Féral, Jean-Pierre Olenin, Sergej Šiaulys, Andrius Somerfield, Paul J. Trayanova, Antoaneta Uyarra, Maria C. Chenuil, Anne 2018 application/pdf http://ku.oai.elaba.lt/documents/31202685.pdf http://ku.lvb.lt/KU:ELABAPDB31202685&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.4283 http://ku.oai.elaba.lt/documents/31202685.pdf http://ku.lvb.lt/KU:ELABAPDB31202685&prefLang=en_US info:eu-repo/semantics/openAccess Ecology and evolution, Hoboken : Wiley, 2018, vol. 8, no. 17, p. 8908-8920 eISSN 2045-7758 Artificial Substrate Unit (ASU) COI Innovative monitoring Marine invertebrates Metabarcoding info:eu-repo/semantics/article 2018 ftlitinstagrecon https://doi.org/10.1002/ece3.4283 2021-12-02T00:58:30Z In a world of declining biodiversity, monitoring is becoming crucial. Molecular methods, such as metabarcoding, have the potential to rapidly expand our knowledge of biodiversity, supporting assessment, management, and conservation. In the marine environment, where hard substrata are more difficult to access than soft bottoms for quantitative ecological studies, Artificial Substrate Units (ASUs) allow for standardized sampling. We deployed ASUs within five regional seas (Baltic Sea, Northeast Atlantic Ocean, Mediterranean Sea, Black Sea, and Red Sea) for 12–26 months to measure the diversity and community composition of macroinvertebrates. We identified invertebrates using a traditional approach based on morphological characters, and by metabarcoding of the mitochondrial cytochrome oxidase I (COI) gene. We compared community composition and diversity metrics obtained using the two methods. Diversity was significantly correlated between data types. Metabarcoding of ASUs allowed for robust comparisons of community composition and diversity, but not all groups were successfully sequenced. All locations were significantly different in taxonomic composition as measured with both kinds of data. We recovered previously known regional biogeographical patterns in both datasets (e.g., low species diversity in the Black and Baltic Seas, affinity between the Bay of Biscay and the Mediterranean). We conclude that the two approaches provide complementary information and that metabarcoding shows great promise for marine monitoring. However, until its pitfalls are addressed, the use of metabarcoding in monitoring of rocky benthic assemblages should be used in addition to classical approaches rather than instead of them. Article in Journal/Newspaper Northeast Atlantic LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Ecology and Evolution 8 17 8908 8920 |
institution |
Open Polar |
collection |
LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
English |
topic |
Artificial Substrate Unit (ASU) COI Innovative monitoring Marine invertebrates Metabarcoding |
spellingShingle |
Artificial Substrate Unit (ASU) COI Innovative monitoring Marine invertebrates Metabarcoding Cahill, Abigail E. Pearman, John K. Borja, Angel Carugati, Laura Carvalho, Susana Danovaro, Roberto Dashfield, Sarah David, Romain Féral, Jean-Pierre Olenin, Sergej Šiaulys, Andrius Somerfield, Paul J. Trayanova, Antoaneta Uyarra, Maria C. Chenuil, Anne A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
topic_facet |
Artificial Substrate Unit (ASU) COI Innovative monitoring Marine invertebrates Metabarcoding |
description |
In a world of declining biodiversity, monitoring is becoming crucial. Molecular methods, such as metabarcoding, have the potential to rapidly expand our knowledge of biodiversity, supporting assessment, management, and conservation. In the marine environment, where hard substrata are more difficult to access than soft bottoms for quantitative ecological studies, Artificial Substrate Units (ASUs) allow for standardized sampling. We deployed ASUs within five regional seas (Baltic Sea, Northeast Atlantic Ocean, Mediterranean Sea, Black Sea, and Red Sea) for 12–26 months to measure the diversity and community composition of macroinvertebrates. We identified invertebrates using a traditional approach based on morphological characters, and by metabarcoding of the mitochondrial cytochrome oxidase I (COI) gene. We compared community composition and diversity metrics obtained using the two methods. Diversity was significantly correlated between data types. Metabarcoding of ASUs allowed for robust comparisons of community composition and diversity, but not all groups were successfully sequenced. All locations were significantly different in taxonomic composition as measured with both kinds of data. We recovered previously known regional biogeographical patterns in both datasets (e.g., low species diversity in the Black and Baltic Seas, affinity between the Bay of Biscay and the Mediterranean). We conclude that the two approaches provide complementary information and that metabarcoding shows great promise for marine monitoring. However, until its pitfalls are addressed, the use of metabarcoding in monitoring of rocky benthic assemblages should be used in addition to classical approaches rather than instead of them. |
format |
Article in Journal/Newspaper |
author |
Cahill, Abigail E. Pearman, John K. Borja, Angel Carugati, Laura Carvalho, Susana Danovaro, Roberto Dashfield, Sarah David, Romain Féral, Jean-Pierre Olenin, Sergej Šiaulys, Andrius Somerfield, Paul J. Trayanova, Antoaneta Uyarra, Maria C. Chenuil, Anne |
author_facet |
Cahill, Abigail E. Pearman, John K. Borja, Angel Carugati, Laura Carvalho, Susana Danovaro, Roberto Dashfield, Sarah David, Romain Féral, Jean-Pierre Olenin, Sergej Šiaulys, Andrius Somerfield, Paul J. Trayanova, Antoaneta Uyarra, Maria C. Chenuil, Anne |
author_sort |
Cahill, Abigail E. |
title |
A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
title_short |
A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
title_full |
A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
title_fullStr |
A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
title_full_unstemmed |
A comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
title_sort |
comparative analysis of metabarcoding and morphology-based identification of benthic communities across different regional seas |
publishDate |
2018 |
url |
http://ku.oai.elaba.lt/documents/31202685.pdf http://ku.lvb.lt/KU:ELABAPDB31202685&prefLang=en_US |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Ecology and evolution, Hoboken : Wiley, 2018, vol. 8, no. 17, p. 8908-8920 eISSN 2045-7758 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/ece3.4283 http://ku.oai.elaba.lt/documents/31202685.pdf http://ku.lvb.lt/KU:ELABAPDB31202685&prefLang=en_US |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/ece3.4283 |
container_title |
Ecology and Evolution |
container_volume |
8 |
container_issue |
17 |
container_start_page |
8908 |
op_container_end_page |
8920 |
_version_ |
1766143242190979072 |