A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus
This study compares the sensitivity of two methods for diagnosing avian haemosporidian parasite (Plasmodium, Haemoproteus and Leucocytozoon) prevalence in Siberian tits Poecile cinctus breeding in northern Finland: a polymerase chain reaction (PCR)-based method and microscopy examination of blood sm...
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ftlitinstagrecon:oai:elaba:3089097 2023-05-15T15:05:46+02:00 A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus Krams, Indrikis Suraka, Valērija Cīrule, Dina Hukkanen, Markku Tummeleht, Lea Mierauskas, Pranas Rytkonen, Seppo Rantala, Markus J. Vrublevska, Jolanta Orell, Markku Krama, Tatjana 2012 http://mru.lvb.lt/MRU:ELABAPDB3089097&prefLang=en_US lit eng lit eng http://mru.lvb.lt/MRU:ELABAPDB3089097&prefLang=en_US Annales Zoologici Fennici, Helsinki : Finnish Zoological and Botanical Publishing Board, 2012, Vol. 49, no. 5-6, p. 331-340 ISSN 0003-455X Siberian tit Haemosporidinian parasites info:eu-repo/semantics/article 2012 ftlitinstagrecon 2021-12-02T00:12:00Z This study compares the sensitivity of two methods for diagnosing avian haemosporidian parasite (Plasmodium, Haemoproteus and Leucocytozoon) prevalence in Siberian tits Poecile cinctus breeding in northern Finland: a polymerase chain reaction (PCR)-based method and microscopy examination of blood smears. During molecular analysis, we used PCR screening and RFLP as a post-PCR diagnostic step. PCR screening detected haemosporidian parasites in the blood of 38 out of 40 (95%) breeding Siberian tits. Microscopy examination of blood smears and PCR diagnostics showed the same prevalences for Leucocytozoon spp. infections. In contrast, prevalences of Haemoproteus spp. and Plasmodium spp. determined by molecular methods were significantly higher than the prevalence determined by microscopy screening. However, habitat and sex effects on the total count of parasites, Plasmodium spp., Haemoproteus spp. and Leucocytozoon spp. counts appeared to be similar when assessed with either screening method, giving the same interpretation for the ecological hypotheses tested. Finally, this study shows that transmission of avian malaria can occur in the area of the Arctic Circle in northern Europe. Article in Journal/Newspaper Arctic Northern Finland LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) Arctic |
institution |
Open Polar |
collection |
LAEI VL (Lithuanian Institute of Agrarian Economics Virtual Library) |
op_collection_id |
ftlitinstagrecon |
language |
Lithuanian English |
topic |
Siberian tit Haemosporidinian parasites |
spellingShingle |
Siberian tit Haemosporidinian parasites Krams, Indrikis Suraka, Valērija Cīrule, Dina Hukkanen, Markku Tummeleht, Lea Mierauskas, Pranas Rytkonen, Seppo Rantala, Markus J. Vrublevska, Jolanta Orell, Markku Krama, Tatjana A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
topic_facet |
Siberian tit Haemosporidinian parasites |
description |
This study compares the sensitivity of two methods for diagnosing avian haemosporidian parasite (Plasmodium, Haemoproteus and Leucocytozoon) prevalence in Siberian tits Poecile cinctus breeding in northern Finland: a polymerase chain reaction (PCR)-based method and microscopy examination of blood smears. During molecular analysis, we used PCR screening and RFLP as a post-PCR diagnostic step. PCR screening detected haemosporidian parasites in the blood of 38 out of 40 (95%) breeding Siberian tits. Microscopy examination of blood smears and PCR diagnostics showed the same prevalences for Leucocytozoon spp. infections. In contrast, prevalences of Haemoproteus spp. and Plasmodium spp. determined by molecular methods were significantly higher than the prevalence determined by microscopy screening. However, habitat and sex effects on the total count of parasites, Plasmodium spp., Haemoproteus spp. and Leucocytozoon spp. counts appeared to be similar when assessed with either screening method, giving the same interpretation for the ecological hypotheses tested. Finally, this study shows that transmission of avian malaria can occur in the area of the Arctic Circle in northern Europe. |
format |
Article in Journal/Newspaper |
author |
Krams, Indrikis Suraka, Valērija Cīrule, Dina Hukkanen, Markku Tummeleht, Lea Mierauskas, Pranas Rytkonen, Seppo Rantala, Markus J. Vrublevska, Jolanta Orell, Markku Krama, Tatjana |
author_facet |
Krams, Indrikis Suraka, Valērija Cīrule, Dina Hukkanen, Markku Tummeleht, Lea Mierauskas, Pranas Rytkonen, Seppo Rantala, Markus J. Vrublevska, Jolanta Orell, Markku Krama, Tatjana |
author_sort |
Krams, Indrikis |
title |
A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
title_short |
A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
title_full |
A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
title_fullStr |
A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
title_full_unstemmed |
A comparison of microscopy and PCR diagnostics for low intensity infections of haemosporidian parasites in the Siberian tit Poecile cinctus |
title_sort |
comparison of microscopy and pcr diagnostics for low intensity infections of haemosporidian parasites in the siberian tit poecile cinctus |
publishDate |
2012 |
url |
http://mru.lvb.lt/MRU:ELABAPDB3089097&prefLang=en_US |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Northern Finland |
genre_facet |
Arctic Northern Finland |
op_source |
Annales Zoologici Fennici, Helsinki : Finnish Zoological and Botanical Publishing Board, 2012, Vol. 49, no. 5-6, p. 331-340 ISSN 0003-455X |
op_relation |
http://mru.lvb.lt/MRU:ELABAPDB3089097&prefLang=en_US |
_version_ |
1766337414338445312 |