Cross-species applicability of microsatellite Markers for investigation of sea ducks (Mergini) genetic differentiation

• Main Authors: Sruoga, Aniolas, Slavėnaitė, Sigita, Butkauskas, Dalius, Gražulevičius, Gediminas
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2008
• Subjects: Anseriformes; Sea ducks; Microsatellites; Genetic variability; Melanitta fusca; velvet scoter
• Online Access: http://lmavb.lvb.lt/LMAVB:ELABAPDB5864410&prefLang=en_US

Genetic studies of waterfowl have provided insufficient information on the evolutionary history of the sea duck tribe (Mergini, Anseriformes), as highly variable molecular markers have not been identified. Cross-species applicability of microsatellites has been shown for several bird families. Therefore, the objective of our work was to examine whether specific primers used previously for Anatidae, Phasianidae and Laridae taxons could amplify microsatellite loci of sea duck species: Long-tailed duck (Clangula hyemalis), Goosander (Mergus merganser) and Velvet Scoter (Melanitta fusca). Tissue samples were collected and DNA was extracted by rapid salt extraction method. Amplification of DNA fragments was carried out using specific microsatellite primers of APH21, Aalmu1, Sfimu4, Sfimu5 (Anatidae), ADL209, ADL115 (Phasianidae) and K71, RGB28 (Laridae). Four primer pairs (APH21, Aalmu1, K71, and nSfimu4) were suitable for investigation of interspecies genetic variability among Long-tailed duck and Velvet Scoter. Intraspecies specificity has been detected for primer pair ADL 209 in all three duck species. The primer pair APH21 was selected as most promising for investigation of intraspecies variability of Long-tailed duck and Velvet Scoter.