Current knowledge about European eel Anguilla anguilla (L.) mtDNA D-loop region haplotypic variety

• Published in: Acta Zoologica Lituanica
• Main Authors: Butkauskas, Dalius, Ragauskas, Adomas, Sruoga, Aniolas, Ložys, Linas, Tzeng, Wann-Nian
• Format: Article in Journal/Newspaper
• Language: Lithuanian; English
• Published: 2009
• Subjects: Anguilla-anguilla; European eel; MtDNA; D-loop; Haplotype; Panmixia; IBD; IBT; Anguilla anguilla
• Online Access: http://lmavb.lvb.lt/LMAVB:ELABAPDB5854459&prefLang=en_US

Today, there are only two information sources about Anguilla anguilla mtDNA D-loop region haplotypic variety and both are quite old. Our research group updated the ten year-old mtDNA D-loop region database of 80 sequences by adding the homological sequences of 45 European eels caught in Lithuanian territorial waters, i.e., in the Baltic Sea, the Curonian Lagoon, and in inland Lakes Dringis and Siesartis. Even 37 unique haplotypes of European eel were found. Based on all available mtDNA D-loop sequence data, 115 polymorphic sites (S), 132 mutations (η), 0.9965 (± 0.002) overall haplotype diversity (H) and 0.02523 nucleotide diversity (π) were determinated. No significant clustering related to the sampling location was identified after the evaluation of the distribution patterns of different haplotypes in the phylogenetic tree constructed using 125 European eel mtDNA D-loop sequences. However, limited genetic differentiation based on geographic distribution of some haplogroups of A. anguilla became evident after the refinement of haplotype relationships using a network approach. The ambiguous clustering of specimens representing geographically related sampling locations in the phylogenetic tree derived using the neighbor-joining method and a non-random distribution of some haplotypes in the median joining network are discussed.