Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain
Background: Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different popu...
Published in: | Parasites & Vectors |
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ftlithuaniansrc:oai:elaba:54924885 2023-05-15T14:31:14+02:00 Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain Kirillova, Viktorija Prakas, Petras Calero-Bernal, Rafael Gavarane, Inese Fernández-García, José Luis Martínez-González, Manuel Rudaitytė-Lukošienė, Eglė Martínez-Estéllez, Miguel Ángel Habela Butkauskas, Dalius Kirjušina, Muza 2018 application/pdf http://gtc.oai.elaba.lt/documents/54924885.pdf http://gtc.lvb.lt/GTC:ELABAPDB54924885&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-018-2694-y http://gtc.oai.elaba.lt/documents/54924885.pdf http://gtc.lvb.lt/GTC:ELABAPDB54924885&prefLang=en_US info:eu-repo/semantics/openAccess Parasites and vectors, London : BioMed Central Ltd., 2018, vol. 11, iss. 1, art. no. 173, p. 299-304 eISSN 1756-3305 Haplotypes Molecular characterisation Phylogeny Sarcocystis arctica Sarcocystis lutrae Wild carnivores info:eu-repo/semantics/article 2018 ftlithuaniansrc https://doi.org/10.1186/s13071-018-2694-y 2021-12-02T00:46:50Z Background: Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different populations in Latvia, Lithuania and Spain. Methods: Muscle samples from 411 red foxes (Vulpes vulpes) and 269 racoon dogs (Nyctereutes procyonoides) from Latvia, 41 red foxes from Lithuania and 22 red foxes from Spain were examined for the presence of Sarcocystis sarcocysts by light microscopy (LM). Sarcocystis spp. were identified by transmission electron microscopy (TEM) and molecular biology techniques. Results: Sarcocystis cysts were detected in 11/411 (2.7%) Latvian, 3/41 (7.3%) Lithuanian, and 6/22 (27.3%) Spanish red foxes, however, cysts were not observed in the muscles of racoon dogs. Based on LM, TEM, 18S rDNA, 28S rDNA, ITS1, cox1 and rpoB sequences, Sarcocystis arctica and Sarcocystis lutrae cysts were identified in red fox muscles from Latvia and Lithuania, whereas only S. arctica was detected in Spain. The 18S rDNA, 28S rDNA and ITS1 sequences from the 21 isolates of S. arctica from Latvia, Lithuania and Spain were identical. By contrast, two and four haplotypes were determined based on mtDNA cox1 and apicoplast rpoB sequences, respectively. Polymorphisms were not detected between the two isolates of S. lutrae from Latvia and Lithuania. Based on phylogenetic results, S. arctica and S. lutrae were most closely related to Sarcocystis spp. using predatory mammals as intermediate hosts and to Sarcocystis species with a bird-bird life-cycle. Conclusions: Based on current knowledge, the red fox and Arctic fox (Vulpes lagopus) could act as intermediate host for the same two Sarcocystis species. Molecular results suggest the existence of two genetic lineages of S. arctica, and such divergence relies on its geographical distribution but not on their intermediate host species. Article in Journal/Newspaper Arctic Fox Arctic Vulpes lagopus LSRC VL (Lithuanian Social Research Centre Virtual Library) Arctic Parasites & Vectors 11 1 |
institution |
Open Polar |
collection |
LSRC VL (Lithuanian Social Research Centre Virtual Library) |
op_collection_id |
ftlithuaniansrc |
language |
English |
topic |
Haplotypes Molecular characterisation Phylogeny Sarcocystis arctica Sarcocystis lutrae Wild carnivores |
spellingShingle |
Haplotypes Molecular characterisation Phylogeny Sarcocystis arctica Sarcocystis lutrae Wild carnivores Kirillova, Viktorija Prakas, Petras Calero-Bernal, Rafael Gavarane, Inese Fernández-García, José Luis Martínez-González, Manuel Rudaitytė-Lukošienė, Eglė Martínez-Estéllez, Miguel Ángel Habela Butkauskas, Dalius Kirjušina, Muza Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
topic_facet |
Haplotypes Molecular characterisation Phylogeny Sarcocystis arctica Sarcocystis lutrae Wild carnivores |
description |
Background: Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different populations in Latvia, Lithuania and Spain. Methods: Muscle samples from 411 red foxes (Vulpes vulpes) and 269 racoon dogs (Nyctereutes procyonoides) from Latvia, 41 red foxes from Lithuania and 22 red foxes from Spain were examined for the presence of Sarcocystis sarcocysts by light microscopy (LM). Sarcocystis spp. were identified by transmission electron microscopy (TEM) and molecular biology techniques. Results: Sarcocystis cysts were detected in 11/411 (2.7%) Latvian, 3/41 (7.3%) Lithuanian, and 6/22 (27.3%) Spanish red foxes, however, cysts were not observed in the muscles of racoon dogs. Based on LM, TEM, 18S rDNA, 28S rDNA, ITS1, cox1 and rpoB sequences, Sarcocystis arctica and Sarcocystis lutrae cysts were identified in red fox muscles from Latvia and Lithuania, whereas only S. arctica was detected in Spain. The 18S rDNA, 28S rDNA and ITS1 sequences from the 21 isolates of S. arctica from Latvia, Lithuania and Spain were identical. By contrast, two and four haplotypes were determined based on mtDNA cox1 and apicoplast rpoB sequences, respectively. Polymorphisms were not detected between the two isolates of S. lutrae from Latvia and Lithuania. Based on phylogenetic results, S. arctica and S. lutrae were most closely related to Sarcocystis spp. using predatory mammals as intermediate hosts and to Sarcocystis species with a bird-bird life-cycle. Conclusions: Based on current knowledge, the red fox and Arctic fox (Vulpes lagopus) could act as intermediate host for the same two Sarcocystis species. Molecular results suggest the existence of two genetic lineages of S. arctica, and such divergence relies on its geographical distribution but not on their intermediate host species. |
format |
Article in Journal/Newspaper |
author |
Kirillova, Viktorija Prakas, Petras Calero-Bernal, Rafael Gavarane, Inese Fernández-García, José Luis Martínez-González, Manuel Rudaitytė-Lukošienė, Eglė Martínez-Estéllez, Miguel Ángel Habela Butkauskas, Dalius Kirjušina, Muza |
author_facet |
Kirillova, Viktorija Prakas, Petras Calero-Bernal, Rafael Gavarane, Inese Fernández-García, José Luis Martínez-González, Manuel Rudaitytė-Lukošienė, Eglė Martínez-Estéllez, Miguel Ángel Habela Butkauskas, Dalius Kirjušina, Muza |
author_sort |
Kirillova, Viktorija |
title |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_short |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_full |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_fullStr |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_full_unstemmed |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_sort |
identification and genetic characterization of sarcocystis arctica and sarcocystis lutrae in red foxes (vulpes vulpes) from baltic states and spain |
publishDate |
2018 |
url |
http://gtc.oai.elaba.lt/documents/54924885.pdf http://gtc.lvb.lt/GTC:ELABAPDB54924885&prefLang=en_US |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Fox Arctic Vulpes lagopus |
genre_facet |
Arctic Fox Arctic Vulpes lagopus |
op_source |
Parasites and vectors, London : BioMed Central Ltd., 2018, vol. 11, iss. 1, art. no. 173, p. 299-304 eISSN 1756-3305 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-018-2694-y http://gtc.oai.elaba.lt/documents/54924885.pdf http://gtc.lvb.lt/GTC:ELABAPDB54924885&prefLang=en_US |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s13071-018-2694-y |
container_title |
Parasites & Vectors |
container_volume |
11 |
container_issue |
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1766304906356981760 |