Paukščių hemosporidinių parazitų (Haemosporida) transmisijos ir sporogonijos tyrimai
Avian malarian and other haemoproteid parasites (Apicomplexa: Haemosporida) are widly spread among various groups of vertebrates, and are found in almost all continets (except Antarctica). Haemosporids are well investigated parazites, because they cause human malaria (Plasmodium), as well as, severe...
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| Format: | Bachelor Thesis |
| Language: | Lithuanian English |
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Institutional Repository of Vilnius University
2018
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| Online Access: | http://vu.lvb.lt/VU:ELABAETD35761438&prefLang=en_US |
| Summary: | Avian malarian and other haemoproteid parasites (Apicomplexa: Haemosporida) are widly spread among various groups of vertebrates, and are found in almost all continets (except Antarctica). Haemosporids are well investigated parazites, because they cause human malaria (Plasmodium), as well as, severe and even lethal diseases in birds (Haemoproteus). The purpose of this thesis is to identify possible vectors of haemoproteid parazites in Verkiai regional park. The goals of the thesis are: collect biting midges of Culicoides genus (Ceratopogonidae) in Verkiai regional park; identify the species of recently blood-engorged biting midges females; extract salivary glands from blood-engorged biting midges females and prepare their samples for further investigation; detect which of the collected specimens are infected with haemoproteids by using molecular investigation methods; detect natural vectors of haemoproteids using microscopy. A new method was applied preparing this thesis. Before investigating collected specimens for haemoproteid infections with molecular methods, salivary glands were extracted from collected biting midges. Research took plane at Verkiai regional park and in P. B. Šivickis laboratory of parasitology at Nature Research Centre (Vilnius, Lithuania) in 2016. During the time of research, 460 blood-engorged biting midges females of genus Culicoides were collected. A total of 15 different species were identified. Molecular methods revealed that 32 specimens of 7 Culicoides species, were infected with 12 different species of Haemoproteus parazites. Haemoproteus pallidus sporozoites were detected in two samples of salivary glands, which were both extracted from Culicoides kibunensis biting midge. For the first time ever, an insect, in which the parasite naturally completes all of the developmental stages, was detected by combining microscopy with molecular research methods. |
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