Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with...
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ftlithuaniansrc:oai:elaba:2952666 2023-05-15T13:47:28+02:00 Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil Venskutonis, Petras Rimantas Daukšas, Egidijus Sivik, Bjorn 2008 http://ktu.lvb.lt/KTU:ELABAPDB2952666&prefLang=en_US eng eng http://ktu.lvb.lt/KTU:ELABAPDB2952666&prefLang=en_US Food technology and biotechnology, Zagreb : University of Zagreb, 2008, Vol. 46, no. 2, p. 157-163 ISSN 1330-9862 Borage Candida antarctica Supercritical CO2 extraction Entrainer info:eu-repo/semantics/article 2008 ftlithuaniansrc 2021-12-02T00:33:53Z This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa. Article in Journal/Newspaper Antarc* Antarctica LSRC VL (Lithuanian Social Research Centre Virtual Library) |
institution |
Open Polar |
collection |
LSRC VL (Lithuanian Social Research Centre Virtual Library) |
op_collection_id |
ftlithuaniansrc |
language |
English |
topic |
Borage Candida antarctica Supercritical CO2 extraction Entrainer |
spellingShingle |
Borage Candida antarctica Supercritical CO2 extraction Entrainer Venskutonis, Petras Rimantas Daukšas, Egidijus Sivik, Bjorn Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
topic_facet |
Borage Candida antarctica Supercritical CO2 extraction Entrainer |
description |
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa. |
format |
Article in Journal/Newspaper |
author |
Venskutonis, Petras Rimantas Daukšas, Egidijus Sivik, Bjorn |
author_facet |
Venskutonis, Petras Rimantas Daukšas, Egidijus Sivik, Bjorn |
author_sort |
Venskutonis, Petras Rimantas |
title |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_short |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_full |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_fullStr |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_full_unstemmed |
Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil |
title_sort |
use of immobilised lipase from candida antarctica in supercritical fluid extraction of borage (borago officinalis l.) seed oil |
publishDate |
2008 |
url |
http://ktu.lvb.lt/KTU:ELABAPDB2952666&prefLang=en_US |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Food technology and biotechnology, Zagreb : University of Zagreb, 2008, Vol. 46, no. 2, p. 157-163 ISSN 1330-9862 |
op_relation |
http://ktu.lvb.lt/KTU:ELABAPDB2952666&prefLang=en_US |
_version_ |
1766247189660565504 |