DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose
We investigated tissue and liver of 24 red deers (Cervus elaphus). Samples for analysis were collected from 4 localities, 8 different forests of Lithuania during 2004-2005 hunting seasons. We used 7 microsatellite primers (RT1, RT23, BM888, NVHRT16, NVHRT21, NVHRT48, NVHRT73) derived from reindeer (...
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Institutional Repository of Vytautas Magnus University
2013
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Online Access: | http://vdu.oai.elaba.lt/documents/1848151.pdf http://vdu.lvb.lt/VDU:ELABAETD1848151&prefLang=en_US |
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ftlithuaniansrc:oai:elaba:1848151 2023-05-15T18:04:21+02:00 DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose The application of microsatellite polymorphism for the genetical variability research of Lithuanian red deer (Cervus elaphus) Jakubanis, Irena Paulauskas, Algimantas info:eu-repo/date/embargoEnd/2013-01-30 application/pdf http://vdu.oai.elaba.lt/documents/1848151.pdf http://vdu.lvb.lt/VDU:ELABAETD1848151&prefLang=en_US lit eng lit eng Institutional Repository of Vytautas Magnus University http://vdu.oai.elaba.lt/documents/1848151.pdf http://vdu.lvb.lt/VDU:ELABAETD1848151&prefLang=en_US info:eu-repo/semantics/embargoedAccess red deer microsatellites populiation genetical variability info:eu-repo/semantics/masterThesis 2013 ftlithuaniansrc 2021-12-02T00:55:37Z We investigated tissue and liver of 24 red deers (Cervus elaphus). Samples for analysis were collected from 4 localities, 8 different forests of Lithuania during 2004-2005 hunting seasons. We used 7 microsatellite primers (RT1, RT23, BM888, NVHRT16, NVHRT21, NVHRT48, NVHRT73) derived from reindeer (Rangifer tarandus) to find out which of them is the best suitable for genetical investigations of red deer. BM888, NVHRT48 and NVHRT21 did not appeared suitable for whole population analysis because not in all samples was detected amplification. The analysis of alleles showed the rarest alleles is RT1 locus 224 bp alleles (0,042). Lithuanian red deers observed and expected heterozygosities are within the range of values previously found for reed deers. It was estimated that the biggest observed heterozygosity were Jurbarko localities forest (Ho= 0,75). The biggest effective number of alleles (ne=3,45) was observed in Panevėžio locality red deer population. According to our DNA and antedate done isoenzyme investigation the most genetically similar were Minčios and Ažvinčių red deer subpopulations; the most different were Sipailiškių and Mantvilių ones. Lithuanian red deer are in one cluster with Hungarian and Scottish red deers populations, they are in separate cluster with Swedish red deer population. Master Thesis Rangifer tarandus LSRC VL (Lithuanian Social Research Centre Virtual Library) |
institution |
Open Polar |
collection |
LSRC VL (Lithuanian Social Research Centre Virtual Library) |
op_collection_id |
ftlithuaniansrc |
language |
Lithuanian English |
topic |
red deer microsatellites populiation genetical variability |
spellingShingle |
red deer microsatellites populiation genetical variability Jakubanis, Irena DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
topic_facet |
red deer microsatellites populiation genetical variability |
description |
We investigated tissue and liver of 24 red deers (Cervus elaphus). Samples for analysis were collected from 4 localities, 8 different forests of Lithuania during 2004-2005 hunting seasons. We used 7 microsatellite primers (RT1, RT23, BM888, NVHRT16, NVHRT21, NVHRT48, NVHRT73) derived from reindeer (Rangifer tarandus) to find out which of them is the best suitable for genetical investigations of red deer. BM888, NVHRT48 and NVHRT21 did not appeared suitable for whole population analysis because not in all samples was detected amplification. The analysis of alleles showed the rarest alleles is RT1 locus 224 bp alleles (0,042). Lithuanian red deers observed and expected heterozygosities are within the range of values previously found for reed deers. It was estimated that the biggest observed heterozygosity were Jurbarko localities forest (Ho= 0,75). The biggest effective number of alleles (ne=3,45) was observed in Panevėžio locality red deer population. According to our DNA and antedate done isoenzyme investigation the most genetically similar were Minčios and Ažvinčių red deer subpopulations; the most different were Sipailiškių and Mantvilių ones. Lithuanian red deer are in one cluster with Hungarian and Scottish red deers populations, they are in separate cluster with Swedish red deer population. |
author2 |
Paulauskas, Algimantas |
format |
Master Thesis |
author |
Jakubanis, Irena |
author_facet |
Jakubanis, Irena |
author_sort |
Jakubanis, Irena |
title |
DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
title_short |
DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
title_full |
DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
title_fullStr |
DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
title_full_unstemmed |
DNR mikrosatelitinio polimorfizno panaudojimas Lietuvos tauriųjų elnių(Cervus elaphus)genetinės įvairovės tyrimuose |
title_sort |
dnr mikrosatelitinio polimorfizno panaudojimas lietuvos tauriųjų elnių(cervus elaphus)genetinės įvairovės tyrimuose |
publisher |
Institutional Repository of Vytautas Magnus University |
publishDate |
2013 |
url |
http://vdu.oai.elaba.lt/documents/1848151.pdf http://vdu.lvb.lt/VDU:ELABAETD1848151&prefLang=en_US |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_relation |
http://vdu.oai.elaba.lt/documents/1848151.pdf http://vdu.lvb.lt/VDU:ELABAETD1848151&prefLang=en_US |
op_rights |
info:eu-repo/semantics/embargoedAccess |
_version_ |
1766175706961674240 |