Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania
The diaphragm muscles of eight sika deer (Cervus nippon) bred in Lithuania were examined for Sarcocystis cysts. Two Sarcocystis species, Sarcocystis taeniata, which were previously reported in Canadian moose (Alces alces) and Argentinean red deer (Cervus elaphus), and Sarcocystis pilosa n. sp. were...
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ftlithuaniansrc:oai:elaba:15881285 2023-05-15T13:13:35+02:00 Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania Prakas, Petras Butkauskas, Dalius Rudaitytė, Eglė Kutkienė, Liuda Sruoga, Aniolas Pūraitė, Irma 2016 http://lsmu.lvb.lt/LSMU:ELABAPDB15881285&prefLang=en_US eng eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-5057-7 http://lsmu.lvb.lt/LSMU:ELABAPDB15881285&prefLang=en_US Parasitology research, Berlin : Springer, 2016, vol. 115, iss. 8, p. 3021-3032 ISSN 0932-0113 Deer parasitology Muscle skeletal Sarcocystosis veterinary Sarcocystis Cyclooxygenase 1 Lithuania info:eu-repo/classification/udc/619: 616.99:599.735.3 info:eu-repo/semantics/article 2016 ftlithuaniansrc https://doi.org/10.1007/s00436-016-5057-7 2021-12-02T00:25:32Z The diaphragm muscles of eight sika deer (Cervus nippon) bred in Lithuania were examined for Sarcocystis cysts. Two Sarcocystis species, Sarcocystis taeniata, which were previously reported in Canadian moose (Alces alces) and Argentinean red deer (Cervus elaphus), and Sarcocystis pilosa n. sp. were described using light microscopy (LM), transmission electron microscopy (TEM), 18S ribosomal DNA (rDNA), and subunit I of cytochrome c oxidase (cox1) sequences analysis. By LM, cysts of S. taeniata were 424.8 × 57.9 (200-837 × 30-100) μm in size and had a thin (up to 1 μm) and smooth cyst wall, while short ribbon-like protrusions arising from broadened cone-shaped bases were seen under TEM. Cysts of S. pilosa (by LM) were ribbon-shaped, measured 848.5 × 63.8 (350-1700 × 30-125) μm and had thin 7-8-μm long hair-like protrusions. By TEM, cyst wall was type 7a-like; protrusions arose from 0.3 μm wide dome-shaped base with minute indentations of the parasitophorous vacuolar membrane near it, the surface of protrusions seemed to be smooth, and the ground substance layer was thin (0.18-0.22 μm). The 18S rDNA, in contrast to the cox1, lacked variability to discriminate S. pilosa from closely related Sarcocystis hjorti from the red deer and moose. S. taeniata, but not S. pilosa, showed a considerable intraspecific variation in both genes analyzed. The phylogenetic analyses based on 18S rDNA and cox1 sequences suggest that canids are definitive hosts of both S. taeniata and S. pilosa. This paper represents the first identification of Sarcocystis species in the sika deer by morphological and molecular methods. Article in Journal/Newspaper Alces alces LSRC VL (Lithuanian Social Research Centre Virtual Library) Parasitology Research 115 8 3021 3032 |
institution |
Open Polar |
collection |
LSRC VL (Lithuanian Social Research Centre Virtual Library) |
op_collection_id |
ftlithuaniansrc |
language |
English |
topic |
Deer parasitology Muscle skeletal Sarcocystosis veterinary Sarcocystis Cyclooxygenase 1 Lithuania info:eu-repo/classification/udc/619: 616.99:599.735.3 |
spellingShingle |
Deer parasitology Muscle skeletal Sarcocystosis veterinary Sarcocystis Cyclooxygenase 1 Lithuania info:eu-repo/classification/udc/619: 616.99:599.735.3 Prakas, Petras Butkauskas, Dalius Rudaitytė, Eglė Kutkienė, Liuda Sruoga, Aniolas Pūraitė, Irma Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
topic_facet |
Deer parasitology Muscle skeletal Sarcocystosis veterinary Sarcocystis Cyclooxygenase 1 Lithuania info:eu-repo/classification/udc/619: 616.99:599.735.3 |
description |
The diaphragm muscles of eight sika deer (Cervus nippon) bred in Lithuania were examined for Sarcocystis cysts. Two Sarcocystis species, Sarcocystis taeniata, which were previously reported in Canadian moose (Alces alces) and Argentinean red deer (Cervus elaphus), and Sarcocystis pilosa n. sp. were described using light microscopy (LM), transmission electron microscopy (TEM), 18S ribosomal DNA (rDNA), and subunit I of cytochrome c oxidase (cox1) sequences analysis. By LM, cysts of S. taeniata were 424.8 × 57.9 (200-837 × 30-100) μm in size and had a thin (up to 1 μm) and smooth cyst wall, while short ribbon-like protrusions arising from broadened cone-shaped bases were seen under TEM. Cysts of S. pilosa (by LM) were ribbon-shaped, measured 848.5 × 63.8 (350-1700 × 30-125) μm and had thin 7-8-μm long hair-like protrusions. By TEM, cyst wall was type 7a-like; protrusions arose from 0.3 μm wide dome-shaped base with minute indentations of the parasitophorous vacuolar membrane near it, the surface of protrusions seemed to be smooth, and the ground substance layer was thin (0.18-0.22 μm). The 18S rDNA, in contrast to the cox1, lacked variability to discriminate S. pilosa from closely related Sarcocystis hjorti from the red deer and moose. S. taeniata, but not S. pilosa, showed a considerable intraspecific variation in both genes analyzed. The phylogenetic analyses based on 18S rDNA and cox1 sequences suggest that canids are definitive hosts of both S. taeniata and S. pilosa. This paper represents the first identification of Sarcocystis species in the sika deer by morphological and molecular methods. |
format |
Article in Journal/Newspaper |
author |
Prakas, Petras Butkauskas, Dalius Rudaitytė, Eglė Kutkienė, Liuda Sruoga, Aniolas Pūraitė, Irma |
author_facet |
Prakas, Petras Butkauskas, Dalius Rudaitytė, Eglė Kutkienė, Liuda Sruoga, Aniolas Pūraitė, Irma |
author_sort |
Prakas, Petras |
title |
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
title_short |
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
title_full |
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
title_fullStr |
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
title_full_unstemmed |
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania |
title_sort |
morphological and molecular characterization of sarcocystis taeniata and sarcocystis pilosa n. sp. from the sika deer (cervus nippon) in lithuania |
publishDate |
2016 |
url |
http://lsmu.lvb.lt/LSMU:ELABAPDB15881285&prefLang=en_US |
genre |
Alces alces |
genre_facet |
Alces alces |
op_source |
Parasitology research, Berlin : Springer, 2016, vol. 115, iss. 8, p. 3021-3032 ISSN 0932-0113 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-016-5057-7 http://lsmu.lvb.lt/LSMU:ELABAPDB15881285&prefLang=en_US |
op_doi |
https://doi.org/10.1007/s00436-016-5057-7 |
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Parasitology Research |
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115 |
container_issue |
8 |
container_start_page |
3021 |
op_container_end_page |
3032 |
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1766259260685025280 |