Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers
DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were us...
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ftlithuaniansrc:oai:elaba:15345360 2023-05-15T18:04:21+02:00 Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas 2014 http://vdu.lvb.lt/VDU:ELABAPDB15345360&prefLang=en_US eng eng http://vdu.lvb.lt/VDU:ELABAPDB15345360&prefLang=en_US Вестник охотоведения : научно-практический и теоретический журнал, Балашиха : Российский государственный аграрный заочный университет, 2014, т. 11, № 2, p. 226-230 ISSN 1994-411X mtDNA. D-loop. Microsatellite. Roe deer Capreolus capreolus Genetic diversity info:eu-repo/classification/udc/57 info:eu-repo/semantics/article 2014 ftlithuaniansrc 2021-12-02T00:15:32Z DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were used. Total number of alleles per locus varied from 2 to 9 and number of effective alleles varied from 2,0 to 7,5. Analysis of molecular variance (AMOVA) showed that genetic diversity of the populations yielded 79 % of the total genetic diversity and among populations 21 % of the total genetic diversity. Mitochondrial DNA was amplified using L-Pro and H-493 primers, which amplify a 436 bp fragment from the first hypervariable domain of the mtDNA control region. Six haplotypes and two haplogroups were identified in population of roe deer in Lithuania. A total 41 variable sites were detected in mtDNA variable region. H2, H3, H4, H5 and H6 haplotypes were specific in roe deer from Lithuania. Haplotype diversity Hd = 0.854 and nucleotide diversity Pi = 0.01981 were calculated. Results of DNA markers analysis revealed high level molecular genetic variation in roe deer population in Lithuania. Article in Journal/Newspaper Rangifer tarandus LSRC VL (Lithuanian Social Research Centre Virtual Library) |
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Open Polar |
collection |
LSRC VL (Lithuanian Social Research Centre Virtual Library) |
op_collection_id |
ftlithuaniansrc |
language |
English |
topic |
mtDNA. D-loop. Microsatellite. Roe deer Capreolus capreolus Genetic diversity info:eu-repo/classification/udc/57 |
spellingShingle |
mtDNA. D-loop. Microsatellite. Roe deer Capreolus capreolus Genetic diversity info:eu-repo/classification/udc/57 Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
topic_facet |
mtDNA. D-loop. Microsatellite. Roe deer Capreolus capreolus Genetic diversity info:eu-repo/classification/udc/57 |
description |
DNA markers play a major role in genetic analysis of mammals. The aim of this study was to assess the genetic structure of roe deer in Lithuania using microsatellite and D-loop sequences analysis. Seven microsattelite derived from rein deer (Rangifer tarandus) with published primer sequences were used. Total number of alleles per locus varied from 2 to 9 and number of effective alleles varied from 2,0 to 7,5. Analysis of molecular variance (AMOVA) showed that genetic diversity of the populations yielded 79 % of the total genetic diversity and among populations 21 % of the total genetic diversity. Mitochondrial DNA was amplified using L-Pro and H-493 primers, which amplify a 436 bp fragment from the first hypervariable domain of the mtDNA control region. Six haplotypes and two haplogroups were identified in population of roe deer in Lithuania. A total 41 variable sites were detected in mtDNA variable region. H2, H3, H4, H5 and H6 haplotypes were specific in roe deer from Lithuania. Haplotype diversity Hd = 0.854 and nucleotide diversity Pi = 0.01981 were calculated. Results of DNA markers analysis revealed high level molecular genetic variation in roe deer population in Lithuania. |
format |
Article in Journal/Newspaper |
author |
Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas |
author_facet |
Pūraitė, Irma Paulauskas, Algimantas Sruoga, Aniolas |
author_sort |
Pūraitė, Irma |
title |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_short |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_full |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_fullStr |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_full_unstemmed |
Genetic analysis of roe deer (Capreolus capreolus L.) using DNA markers |
title_sort |
genetic analysis of roe deer (capreolus capreolus l.) using dna markers |
publishDate |
2014 |
url |
http://vdu.lvb.lt/VDU:ELABAPDB15345360&prefLang=en_US |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Вестник охотоведения : научно-практический и теоретический журнал, Балашиха : Российский государственный аграрный заочный университет, 2014, т. 11, № 2, p. 226-230 ISSN 1994-411X |
op_relation |
http://vdu.lvb.lt/VDU:ELABAPDB15345360&prefLang=en_US |
_version_ |
1766175710268882944 |