Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom
Bacterioplankton provide important ecosystem functions by carrying out biogeochemical cycling of organic matter. Playing an important role in the microbial loop they help remineralize carbon and nutrients. Bacteria also interact with phytoplankton during phytoplankton blooms. However, fundamental un...
Main Author: | |
---|---|
Format: | Bachelor Thesis |
Language: | English |
Published: |
Linnéuniversitetet, Institutionen för biologi och miljö (BOM)
2018
|
Subjects: | |
Online Access: | http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-79200 |
id |
ftlinnaeusuniv:oai:DiVA.org:lnu-79200 |
---|---|
record_format |
openpolar |
spelling |
ftlinnaeusuniv:oai:DiVA.org:lnu-79200 2023-05-15T17:38:37+02:00 Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom Amnebrink, Dennis 2018 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-79200 eng eng Linnéuniversitetet, Institutionen för biologi och miljö (BOM) http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-79200 info:eu-repo/semantics/openAccess Transcriptomics bacteria microbes DOM marine environment bioinformatics Other Biological Topics Annan biologi Bioinformatics and Systems Biology Bioinformatik och systembiologi Student thesis info:eu-repo/semantics/bachelorThesis text 2018 ftlinnaeusuniv 2022-11-03T15:51:20Z Bacterioplankton provide important ecosystem functions by carrying out biogeochemical cycling of organic matter. Playing an important role in the microbial loop they help remineralize carbon and nutrients. Bacteria also interact with phytoplankton during phytoplankton blooms. However, fundamental understanding on the underlying molecular mechanisms involved in the degradation of phytoplankton-derived organic matter is still in its infancy. Therefore, we analysed data from a mesocosm experiment following a natural phytoplankton-bloom from an upwelling system in the North- East Atlantic Ocean. The purpose was to contribute a mechanistic understanding based on functional gene expression analysis of natural microbial assemblages. Our results show the difference in functional gene expression within a bacterial metacommunity and how this functional response drastically switches between bloom build up and senescence. Transcripts showed a broad change in gene expression involving major SEED categories, with the bloom senescence phase exhibiting a higher relative abundance in major categories such as Carbohydrates, Protein Metabolism and Amino Acids and Derivatives. Within these categories genes connected to carbon utilization and transport systems (Ton and Tol) as well as chemotaxis showed a higher abundance during bloom senescence. The change in functionality based on transcripts showed a different bacterial community composition appearing over a very short time. We thus conclude that the bacterial functional gene expression response between build-up and degradation bloom phases is remarkably different and associated with a change in the identity of bacteria with active expression. Our findings highlight the importance of bacterial substrate specialists with different functional roles during different time points of phytoplankton blooms. Bachelor Thesis North East Atlantic Linnaeus University Kalmar Växjö: Publications (DiVA) |
institution |
Open Polar |
collection |
Linnaeus University Kalmar Växjö: Publications (DiVA) |
op_collection_id |
ftlinnaeusuniv |
language |
English |
topic |
Transcriptomics bacteria microbes DOM marine environment bioinformatics Other Biological Topics Annan biologi Bioinformatics and Systems Biology Bioinformatik och systembiologi |
spellingShingle |
Transcriptomics bacteria microbes DOM marine environment bioinformatics Other Biological Topics Annan biologi Bioinformatics and Systems Biology Bioinformatik och systembiologi Amnebrink, Dennis Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
topic_facet |
Transcriptomics bacteria microbes DOM marine environment bioinformatics Other Biological Topics Annan biologi Bioinformatics and Systems Biology Bioinformatik och systembiologi |
description |
Bacterioplankton provide important ecosystem functions by carrying out biogeochemical cycling of organic matter. Playing an important role in the microbial loop they help remineralize carbon and nutrients. Bacteria also interact with phytoplankton during phytoplankton blooms. However, fundamental understanding on the underlying molecular mechanisms involved in the degradation of phytoplankton-derived organic matter is still in its infancy. Therefore, we analysed data from a mesocosm experiment following a natural phytoplankton-bloom from an upwelling system in the North- East Atlantic Ocean. The purpose was to contribute a mechanistic understanding based on functional gene expression analysis of natural microbial assemblages. Our results show the difference in functional gene expression within a bacterial metacommunity and how this functional response drastically switches between bloom build up and senescence. Transcripts showed a broad change in gene expression involving major SEED categories, with the bloom senescence phase exhibiting a higher relative abundance in major categories such as Carbohydrates, Protein Metabolism and Amino Acids and Derivatives. Within these categories genes connected to carbon utilization and transport systems (Ton and Tol) as well as chemotaxis showed a higher abundance during bloom senescence. The change in functionality based on transcripts showed a different bacterial community composition appearing over a very short time. We thus conclude that the bacterial functional gene expression response between build-up and degradation bloom phases is remarkably different and associated with a change in the identity of bacteria with active expression. Our findings highlight the importance of bacterial substrate specialists with different functional roles during different time points of phytoplankton blooms. |
format |
Bachelor Thesis |
author |
Amnebrink, Dennis |
author_facet |
Amnebrink, Dennis |
author_sort |
Amnebrink, Dennis |
title |
Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
title_short |
Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
title_full |
Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
title_fullStr |
Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
title_full_unstemmed |
Transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
title_sort |
transcriptomic profiling of marine bacteria between development and senescence phases of a phytoplankton bloom |
publisher |
Linnéuniversitetet, Institutionen för biologi och miljö (BOM) |
publishDate |
2018 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-79200 |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_relation |
http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-79200 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766139136303955968 |