Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica.
INTRODUCTION: The genus Brachyspira contains well-known enteric pathogens of veterinary significance, suggested agents of colonic disease in humans, and one potentially zoonotic agent. There are recent studies showing that Brachyspira are more widespread in the wildlife community than previously tho...
Published in: | Infection Ecology & Epidemiology |
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Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, Sweden
2015
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Subjects: | |
Online Access: | http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180610 https://doi.org/10.3402/iee.v5.29296 |
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ftlinkoepinguniv:oai:DiVA.org:liu-180610 |
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openpolar |
institution |
Open Polar |
collection |
LIU - Linköping University: Publications (DiVA) |
op_collection_id |
ftlinkoepinguniv |
language |
English |
topic |
Antarctica Brachyspira antimicrobial susceptibility brown skua intestinal spirochaete kelp goose phylogeny snowy sheathbill southern Atlantic region sub-Antarctic islands Microbiology Mikrobiologi |
spellingShingle |
Antarctica Brachyspira antimicrobial susceptibility brown skua intestinal spirochaete kelp goose phylogeny snowy sheathbill southern Atlantic region sub-Antarctic islands Microbiology Mikrobiologi Jansson, Désirée S Mushtaq, Memoona Johansson, Karl-Erik Bonnedahl, Jonas Waldenström, Jonas Andersson, Dan I Broman, Tina Berg, Charlotte Olsen, Björn Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
topic_facet |
Antarctica Brachyspira antimicrobial susceptibility brown skua intestinal spirochaete kelp goose phylogeny snowy sheathbill southern Atlantic region sub-Antarctic islands Microbiology Mikrobiologi |
description |
INTRODUCTION: The genus Brachyspira contains well-known enteric pathogens of veterinary significance, suggested agents of colonic disease in humans, and one potentially zoonotic agent. There are recent studies showing that Brachyspira are more widespread in the wildlife community than previously thought. There are no records of this genus in wildlife from the southern Atlantic region and Antarctica. Our aim was therefore, to determine whether intestinal spirochaetes of genus Brachyspira colonise marine and coastal birds in this region. METHOD: Faecal samples were collected from marine and coastal birds in the southern Atlantic region, including sub-Antarctic islands and Antarctica, in 2002, 2009, and 2012, with the aim to isolate and characterise zoonotic agents. In total, 205 samples from 11 bird species were selectively cultured for intestinal spirochaetes of genus Brachyspira. To identify isolates to species level, they were subjected to phenotyping, species-specific polymerase chain reactions, sequencing of partial 16S rRNA, NADH oxidase (nox), and tlyA genes, and phylogenetic analysis. Antimicrobial susceptibility tests were performed. RESULTS: Fourteen unique strains were obtained from 10 birds of three species: four snowy sheathbills (Chionis albus), three kelp geese (Chloephaga hybrida subsp. malvinarum), and three brown skua (Stercorarius antarcticus subsp. lonnbergi) sampled on the Falkland Islands, Tierra del Fuego in Argentina, South Georgia, South Shetland Islands, and the Antarctic Peninsula. Five Brachyspira strains were closely related to potentially enteropathogenic Brachyspira sp. of chickens: B. intermedia (n=2, from snowy sheathbills), and B. alvinipulli (n=3, from a kelp goose and two snowy sheathbills). Three strains from kelp geese were most similar to the presumed non-pathogenic species 'B. pulli' and B. murdochii, whereas the remaining six strains could not be attributed to currently known species. No isolates related to human strains were found. None of the tested strains showed decreased susceptibility to tiamulin, valnemulin, doxycycline, tylvalosin, lincomycin, or tylosin. CONCLUSIONS: This is the first report of intestinal spirochaetes from this region. Despite limitations of current diagnostic methods, our results, together with earlier studies, show that Brachyspira spp., including potentially pathogenic strains, occur globally among free-living avian hosts, and that this genus encompasses a higher degree of biodiversity than previously recognised. |
format |
Article in Journal/Newspaper |
author |
Jansson, Désirée S Mushtaq, Memoona Johansson, Karl-Erik Bonnedahl, Jonas Waldenström, Jonas Andersson, Dan I Broman, Tina Berg, Charlotte Olsen, Björn |
author_facet |
Jansson, Désirée S Mushtaq, Memoona Johansson, Karl-Erik Bonnedahl, Jonas Waldenström, Jonas Andersson, Dan I Broman, Tina Berg, Charlotte Olsen, Björn |
author_sort |
Jansson, Désirée S |
title |
Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
title_short |
Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
title_full |
Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
title_fullStr |
Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
title_full_unstemmed |
Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. |
title_sort |
intestinal spirochaetes (genus brachyspira) colonise wild birds in the southern atlantic region and antarctica. |
publisher |
Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, Sweden |
publishDate |
2015 |
url |
http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180610 https://doi.org/10.3402/iee.v5.29296 |
long_lat |
ENVELOPE(-60.633,-60.633,-63.883,-63.883) ENVELOPE(25.750,25.750,66.500,66.500) |
geographic |
Antarctic The Antarctic Antarctic Peninsula South Shetland Islands Argentina Chionis Pulli |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula South Shetland Islands Argentina Chionis Pulli |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica antarcticus Brown Skua Chionis albus Snowy Sheathbill South Shetland Islands Stercorarius antarcticus Tierra del Fuego |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica antarcticus Brown Skua Chionis albus Snowy Sheathbill South Shetland Islands Stercorarius antarcticus Tierra del Fuego |
op_relation |
Infection Ecology & Epidemiology, 2000-8686, 2015, 5:1, http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180610 doi:10.3402/iee.v5.29296 PMID 26584828 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3402/iee.v5.29296 |
container_title |
Infection Ecology & Epidemiology |
container_volume |
5 |
container_issue |
1 |
container_start_page |
29296 |
_version_ |
1766258759193067520 |
spelling |
ftlinkoepinguniv:oai:DiVA.org:liu-180610 2023-05-15T13:53:34+02:00 Intestinal spirochaetes (genus Brachyspira) colonise wild birds in the southern Atlantic region and Antarctica. Jansson, Désirée S Mushtaq, Memoona Johansson, Karl-Erik Bonnedahl, Jonas Waldenström, Jonas Andersson, Dan I Broman, Tina Berg, Charlotte Olsen, Björn 2015 application/pdf http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180610 https://doi.org/10.3402/iee.v5.29296 eng eng Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, Sweden Department of Animal Breeding and Genetics, Global Bioinformatics Centre, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden Department of Biomedical Sciences and Veterinary Public HealthSwedish University of Agricultural Sciences, Uppsala, Sweden Department of Infectious Diseases, Kalmar County Hospital, Kalmar, Sweden; Centre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden Centre for Ecology and Evolution in Microbial Model Systems, Linnaeus University, Kalmar, Sweden Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden Division of CBRN Defence and SecurityFOI – Swedish Defence Research Agency, Umeå, Sweden Department of Animal Environment and HealthSwedish University of Agricultural Sciences, Skara, Sweden Section of Infectious Diseases Department of Medical Sciences, Uppsala University, Uppsala, Sweden Infection Ecology & Epidemiology, 2000-8686, 2015, 5:1, http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-180610 doi:10.3402/iee.v5.29296 PMID 26584828 info:eu-repo/semantics/openAccess Antarctica Brachyspira antimicrobial susceptibility brown skua intestinal spirochaete kelp goose phylogeny snowy sheathbill southern Atlantic region sub-Antarctic islands Microbiology Mikrobiologi Article in journal info:eu-repo/semantics/article text 2015 ftlinkoepinguniv https://doi.org/10.3402/iee.v5.29296 2022-05-01T08:24:34Z INTRODUCTION: The genus Brachyspira contains well-known enteric pathogens of veterinary significance, suggested agents of colonic disease in humans, and one potentially zoonotic agent. There are recent studies showing that Brachyspira are more widespread in the wildlife community than previously thought. There are no records of this genus in wildlife from the southern Atlantic region and Antarctica. Our aim was therefore, to determine whether intestinal spirochaetes of genus Brachyspira colonise marine and coastal birds in this region. METHOD: Faecal samples were collected from marine and coastal birds in the southern Atlantic region, including sub-Antarctic islands and Antarctica, in 2002, 2009, and 2012, with the aim to isolate and characterise zoonotic agents. In total, 205 samples from 11 bird species were selectively cultured for intestinal spirochaetes of genus Brachyspira. To identify isolates to species level, they were subjected to phenotyping, species-specific polymerase chain reactions, sequencing of partial 16S rRNA, NADH oxidase (nox), and tlyA genes, and phylogenetic analysis. Antimicrobial susceptibility tests were performed. RESULTS: Fourteen unique strains were obtained from 10 birds of three species: four snowy sheathbills (Chionis albus), three kelp geese (Chloephaga hybrida subsp. malvinarum), and three brown skua (Stercorarius antarcticus subsp. lonnbergi) sampled on the Falkland Islands, Tierra del Fuego in Argentina, South Georgia, South Shetland Islands, and the Antarctic Peninsula. Five Brachyspira strains were closely related to potentially enteropathogenic Brachyspira sp. of chickens: B. intermedia (n=2, from snowy sheathbills), and B. alvinipulli (n=3, from a kelp goose and two snowy sheathbills). Three strains from kelp geese were most similar to the presumed non-pathogenic species 'B. pulli' and B. murdochii, whereas the remaining six strains could not be attributed to currently known species. No isolates related to human strains were found. None of the tested strains showed decreased susceptibility to tiamulin, valnemulin, doxycycline, tylvalosin, lincomycin, or tylosin. CONCLUSIONS: This is the first report of intestinal spirochaetes from this region. Despite limitations of current diagnostic methods, our results, together with earlier studies, show that Brachyspira spp., including potentially pathogenic strains, occur globally among free-living avian hosts, and that this genus encompasses a higher degree of biodiversity than previously recognised. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica antarcticus Brown Skua Chionis albus Snowy Sheathbill South Shetland Islands Stercorarius antarcticus Tierra del Fuego LIU - Linköping University: Publications (DiVA) Antarctic The Antarctic Antarctic Peninsula South Shetland Islands Argentina Chionis ENVELOPE(-60.633,-60.633,-63.883,-63.883) Pulli ENVELOPE(25.750,25.750,66.500,66.500) Infection Ecology & Epidemiology 5 1 29296 |