Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit
N-acetyltransferase 2 (NAT2) is the main enzyme metabolizing isoniazid and genotype-based treatment has been studied for years without becoming common practice. To investigate whether genotype-based isoniazid treatment is feasible in Greenland, we sequenced the coding sequence of NAT2 and determined...
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ftleibnizopen:oai:oai.leibnizopen.de:rY8ByYkBdbrxVwz6IPm5 2023-08-27T04:09:45+02:00 Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit Birch Kristensen, Emilie Yakimov, Victor Bjorn-Mortensen, Karen Soborg, Bolette Koch, Anders Andersson, Mikael Birch Kristensen, Kasper Wilk Michelsen, Sascha Skotte, Line Ahrendt Bjerregaard, Anne Blaszkewicz, Meinolf Golka, Klaus Hengstler, Jan Feenstra, Bjarke Melbye, Mads Geller, Frank 2018 https://repository.publisso.de/resource/frl:6414832 https://doi.org/10.17179/excli2018-1671 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295636/ https://www.excli.de/index.php/excli/article/view/660/1865 eng eng https://creativecommons.org/licenses/by/4.0/ EXCLI journal, 17:1043-1053 Greenland NAT2 genotype status NAT2 enzyme activity N-acetyltransferase 2 caffeine test isoniazid 2018 ftleibnizopen https://doi.org/10.17179/excli2018-1671 2023-08-06T23:33:12Z N-acetyltransferase 2 (NAT2) is the main enzyme metabolizing isoniazid and genotype-based treatment has been studied for years without becoming common practice. To investigate whether genotype-based isoniazid treatment is feasible in Greenland, we sequenced the coding sequence of NAT2 and determined the NAT2 enzyme-activity by caffeine test. No additional genetic variants were identified in the coding sequence of NAT2, so that genotype status in 260 study participants could be assessed by a well-established 7-SNP panel. Studying the enzyme activity by the ratio of the two caffeine metabolites AFMU and 1X in 260 participants showed a high rate of slow phenotypes with intermediate or rapid genotype. These misclassifications were mainly observed in urine samples with pH<3, a deviation from the standard protocol due to the field work character of the study, where immediate pH adjustment to pH=3.5 was not possible. We excluded these samples. For the remaining 143 individuals with pH>3, we observed a moderate level of discrepancies (19 of the 116 individuals with intermediate or rapid genotype status having a slow phenotype). Further investigation showed that drinking coffee and not tea or cola was the most important factor for high levels of both metabolites. The concordance between phenotype and genotype status with regard to slow metabolism supported the recommendation of lower isoniazid doses in individuals with slow genotype status in order to avoid liver injury, a frequent side effect. The phenotypical variation observed for individuals with intermediate or rapid genotype status warrants further research before increased dosing of isoniazid can be recommended. Other/Unknown Material Greenland greenlandic inuit LeibnizOpen (The Leibniz Association) Greenland |
institution |
Open Polar |
collection |
LeibnizOpen (The Leibniz Association) |
op_collection_id |
ftleibnizopen |
language |
English |
topic |
Greenland NAT2 genotype status NAT2 enzyme activity N-acetyltransferase 2 caffeine test isoniazid |
spellingShingle |
Greenland NAT2 genotype status NAT2 enzyme activity N-acetyltransferase 2 caffeine test isoniazid Birch Kristensen, Emilie Yakimov, Victor Bjorn-Mortensen, Karen Soborg, Bolette Koch, Anders Andersson, Mikael Birch Kristensen, Kasper Wilk Michelsen, Sascha Skotte, Line Ahrendt Bjerregaard, Anne Blaszkewicz, Meinolf Golka, Klaus Hengstler, Jan Feenstra, Bjarke Melbye, Mads Geller, Frank Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
topic_facet |
Greenland NAT2 genotype status NAT2 enzyme activity N-acetyltransferase 2 caffeine test isoniazid |
description |
N-acetyltransferase 2 (NAT2) is the main enzyme metabolizing isoniazid and genotype-based treatment has been studied for years without becoming common practice. To investigate whether genotype-based isoniazid treatment is feasible in Greenland, we sequenced the coding sequence of NAT2 and determined the NAT2 enzyme-activity by caffeine test. No additional genetic variants were identified in the coding sequence of NAT2, so that genotype status in 260 study participants could be assessed by a well-established 7-SNP panel. Studying the enzyme activity by the ratio of the two caffeine metabolites AFMU and 1X in 260 participants showed a high rate of slow phenotypes with intermediate or rapid genotype. These misclassifications were mainly observed in urine samples with pH<3, a deviation from the standard protocol due to the field work character of the study, where immediate pH adjustment to pH=3.5 was not possible. We excluded these samples. For the remaining 143 individuals with pH>3, we observed a moderate level of discrepancies (19 of the 116 individuals with intermediate or rapid genotype status having a slow phenotype). Further investigation showed that drinking coffee and not tea or cola was the most important factor for high levels of both metabolites. The concordance between phenotype and genotype status with regard to slow metabolism supported the recommendation of lower isoniazid doses in individuals with slow genotype status in order to avoid liver injury, a frequent side effect. The phenotypical variation observed for individuals with intermediate or rapid genotype status warrants further research before increased dosing of isoniazid can be recommended. |
author |
Birch Kristensen, Emilie Yakimov, Victor Bjorn-Mortensen, Karen Soborg, Bolette Koch, Anders Andersson, Mikael Birch Kristensen, Kasper Wilk Michelsen, Sascha Skotte, Line Ahrendt Bjerregaard, Anne Blaszkewicz, Meinolf Golka, Klaus Hengstler, Jan Feenstra, Bjarke Melbye, Mads Geller, Frank |
author_facet |
Birch Kristensen, Emilie Yakimov, Victor Bjorn-Mortensen, Karen Soborg, Bolette Koch, Anders Andersson, Mikael Birch Kristensen, Kasper Wilk Michelsen, Sascha Skotte, Line Ahrendt Bjerregaard, Anne Blaszkewicz, Meinolf Golka, Klaus Hengstler, Jan Feenstra, Bjarke Melbye, Mads Geller, Frank |
author_sort |
Birch Kristensen, Emilie |
title |
Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
title_short |
Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
title_full |
Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
title_fullStr |
Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
title_full_unstemmed |
Study of correlation between the NAT2 phenotype and genotype status among Greenlandic Inuit |
title_sort |
study of correlation between the nat2 phenotype and genotype status among greenlandic inuit |
publishDate |
2018 |
url |
https://repository.publisso.de/resource/frl:6414832 https://doi.org/10.17179/excli2018-1671 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295636/ https://www.excli.de/index.php/excli/article/view/660/1865 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland greenlandic inuit |
genre_facet |
Greenland greenlandic inuit |
op_source |
EXCLI journal, 17:1043-1053 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.17179/excli2018-1671 |
_version_ |
1775351323617656832 |