A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chroma...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Katlenburg-Lindau : Copernicus Publ.
2020
|
Subjects: | |
Online Access: | https://oa.tib.eu/renate/handle/123456789/6269 https://doi.org/10.34657/5316 |
id |
ftleibnizopen:oai:oai.leibnizopen.de:qhN2DYsBBwLIz6xG5e9f |
---|---|
record_format |
openpolar |
spelling |
ftleibnizopen:oai:oai.leibnizopen.de:qhN2DYsBBwLIz6xG5e9f 2023-11-05T03:39:20+01:00 A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut 2020 application/pdf https://oa.tib.eu/renate/handle/123456789/6269 https://doi.org/10.34657/5316 eng eng Katlenburg-Lindau : Copernicus Publ. CC BY 4.0 Unported https://creativecommons.org/licenses/by/4.0/ Ocean science : OS 16 (2020), Nr. 4 dissolved free (DFCHO) dissolved combined carbohydrates (DCCHO) oceanic seawater electro-dialysis (ED) Arctic ice 550 article Text 2020 ftleibnizopen https://doi.org/10.34657/5316 2023-10-08T23:34:19Z An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. Leibniz_Fonds publishedVersion Article in Journal/Newspaper Arctic ice core LeibnizOpen (The Leibniz Association) |
institution |
Open Polar |
collection |
LeibnizOpen (The Leibniz Association) |
op_collection_id |
ftleibnizopen |
language |
English |
topic |
dissolved free (DFCHO) dissolved combined carbohydrates (DCCHO) oceanic seawater electro-dialysis (ED) Arctic ice 550 |
spellingShingle |
dissolved free (DFCHO) dissolved combined carbohydrates (DCCHO) oceanic seawater electro-dialysis (ED) Arctic ice 550 Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
topic_facet |
dissolved free (DFCHO) dissolved combined carbohydrates (DCCHO) oceanic seawater electro-dialysis (ED) Arctic ice 550 |
description |
An optimized method is presented to determine dissolved free (DFCHO) and dissolved combined carbohydrates (DCCHO) in saline matrices, such as oceanic seawater, Arctic ice core samples or brine using a combination of a desalination with electro-dialysis (ED) and high-performance anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Free neutral sugars, such as glucose and galactose, were found with 95 %–98 % recovery rates. Free amino sugars and free uronic acids were strongly depleted during ED at pH=8, but an adjustment of the pH could result in higher recoveries (58 %–59 % for amino sugars at pH=11; 45 %–49 % for uronic acids at pH=1.5). The applicability of this method for the analysis of DCCHO was evaluated with standard solutions and seawater samples compared with another established desalination method using membrane dialysis. DFCHO in field samples from different regions on Earth ranged between 11 and 118 nM and DCCHO between 260 and 1410 nM. This novel method has the potential to contribute to a better understanding of biogeochemical processes in the oceans and sea–air transfer processes of organic matter into the atmosphere in future studies. Leibniz_Fonds publishedVersion |
format |
Article in Journal/Newspaper |
author |
Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut |
author_facet |
Zeppenfeld, Sebastian van Pinxteren, Manuela Engel, Anja Herrmann, Hartmut |
author_sort |
Zeppenfeld, Sebastian |
title |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_short |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_fullStr |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_full_unstemmed |
A protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ED) – combined with HPAEC-PAD |
title_sort |
protocol for quantifying mono- and polysaccharides in seawater and related saline matrices by electro-dialysis (ed) – combined with hpaec-pad |
publisher |
Katlenburg-Lindau : Copernicus Publ. |
publishDate |
2020 |
url |
https://oa.tib.eu/renate/handle/123456789/6269 https://doi.org/10.34657/5316 |
genre |
Arctic ice core |
genre_facet |
Arctic ice core |
op_source |
Ocean science : OS 16 (2020), Nr. 4 |
op_rights |
CC BY 4.0 Unported https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.34657/5316 |
_version_ |
1781695147977736192 |