Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector?
Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to i...
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ftleibnizopen:oai:oai.leibnizopen.de:iiaKVYsBBwLIz6xGOFON 2023-11-12T04:15:40+01:00 Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? Kutzer, Peter Szentiks, Claudia A. Bock, Sabine Fritsch, Guido Magyar, Tibor Schulze, Christoph Semmler, Torsten Ewers, Christa 2021 https://repository.publisso.de/resource/frl:6434313 https://doi.org/10.3390/microorganisms9091999 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465458/ https://www.mdpi.com/2076-2607/9/9/1999#supplementary eng eng https://creativecommons.org/licenses/by/4.0/ Microorganisms, 9(9):1999 wild boar core genome virulence MLST domestic animals Pasteurella multocida 2021 ftleibnizopen https://doi.org/10.3390/microorganisms9091999 2023-10-22T23:27:56Z Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German PmB-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 PmB isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three PmB isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 PmB-HS-isolates and the three PmB-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122RIRDC and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122RIRDC strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of PmB-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour PmB as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany. Other/Unknown Material Canis lupus Unknown Microorganisms 9 9 1999 |
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collection |
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ftleibnizopen |
language |
English |
topic |
wild boar core genome virulence MLST domestic animals Pasteurella multocida |
spellingShingle |
wild boar core genome virulence MLST domestic animals Pasteurella multocida Kutzer, Peter Szentiks, Claudia A. Bock, Sabine Fritsch, Guido Magyar, Tibor Schulze, Christoph Semmler, Torsten Ewers, Christa Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
topic_facet |
wild boar core genome virulence MLST domestic animals Pasteurella multocida |
description |
Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German PmB-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 PmB isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three PmB isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 PmB-HS-isolates and the three PmB-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122RIRDC and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122RIRDC strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of PmB-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour PmB as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany. |
author |
Kutzer, Peter Szentiks, Claudia A. Bock, Sabine Fritsch, Guido Magyar, Tibor Schulze, Christoph Semmler, Torsten Ewers, Christa |
author_facet |
Kutzer, Peter Szentiks, Claudia A. Bock, Sabine Fritsch, Guido Magyar, Tibor Schulze, Christoph Semmler, Torsten Ewers, Christa |
author_sort |
Kutzer, Peter |
title |
Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
title_short |
Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
title_full |
Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
title_fullStr |
Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
title_full_unstemmed |
Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
title_sort |
re-emergence and spread of haemorrhagic septicaemia in germany: the wolf as a vector? |
publishDate |
2021 |
url |
https://repository.publisso.de/resource/frl:6434313 https://doi.org/10.3390/microorganisms9091999 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465458/ https://www.mdpi.com/2076-2607/9/9/1999#supplementary |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Microorganisms, 9(9):1999 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microorganisms9091999 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
9 |
container_start_page |
1999 |
_version_ |
1782332958424694784 |