Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector?

Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to i...

Full description

Bibliographic Details
Published in:Microorganisms
Main Authors: Kutzer, Peter, Szentiks, Claudia A., Bock, Sabine, Fritsch, Guido, Magyar, Tibor, Schulze, Christoph, Semmler, Torsten, Ewers, Christa
Language:English
Published: 2021
Subjects:
wild boar
core genome
virulence
MLST
domestic animals
Pasteurella multocida
Canis lupus
Online Access:https://repository.publisso.de/resource/frl:6434313
https://doi.org/10.3390/microorganisms9091999
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465458/
https://www.mdpi.com/2076-2607/9/9/1999#supplementary
Description
Summary:Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by Pasteurella (P.) multocida capsular type B (PmB) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (Canis lupus) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German PmB-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 PmB isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three PmB isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 PmB-HS-isolates and the three PmB-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122RIRDC and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122RIRDC strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of PmB-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour PmB as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany.

Similar Items