Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study...
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ftleibnizopen:oai:oai.leibnizopen.de:GdE_fYoBNQPDO7WIRtLS 2023-10-09T21:54:45+02:00 Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe Dafalla, Maysaa Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan Heckel, Gerald Ulrich, Rainer G. 2022 https://repository.publisso.de/resource/frl:6449375 https://doi.org/10.1007/s11262-022-01952-2 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025241/ https://link.springer.com/article/10.1007/s11262-022-01952-2#Sec2 eng eng CC BY 4.0 http://lobid.org/resources/99370676569406441#!, 59(2):323-332 Host specificity Hantavirus Host RT-qPCR Europe Bats 2022 ftleibnizopen https://doi.org/10.1007/s11262-022-01952-2 2023-09-10T23:09:14Z Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. Other/Unknown Material Nyctalus noctula LeibnizOpen (The Leibniz Association) Virus Genes 59 2 323 332 |
institution |
Open Polar |
collection |
LeibnizOpen (The Leibniz Association) |
op_collection_id |
ftleibnizopen |
language |
English |
topic |
Host specificity Hantavirus Host RT-qPCR Europe Bats |
spellingShingle |
Host specificity Hantavirus Host RT-qPCR Europe Bats Dafalla, Maysaa Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan Heckel, Gerald Ulrich, Rainer G. Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
topic_facet |
Host specificity Hantavirus Host RT-qPCR Europe Bats |
description |
Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. |
author |
Dafalla, Maysaa Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan Heckel, Gerald Ulrich, Rainer G. |
author_facet |
Dafalla, Maysaa Orłowska, Anna Keleş, Sinan Julian Straková, Petra Schlottau, Kore Jeske, Kathrin Hoffmann, Bernd Wibbelt, Gudrun Smreczak, Marcin Müller, Thomas Freuling, Conrad Martin Wang, Xuejing Rola, Jerzy Drewes, Stephan Fereidouni, Sasan Heckel, Gerald Ulrich, Rainer G. |
author_sort |
Dafalla, Maysaa |
title |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_short |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_full |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_fullStr |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_full_unstemmed |
Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe |
title_sort |
hantavirus brno loanvirus is highly specific to the common noctule bat (nyctalus noctula) and widespread in central europe |
publishDate |
2022 |
url |
https://repository.publisso.de/resource/frl:6449375 https://doi.org/10.1007/s11262-022-01952-2 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025241/ https://link.springer.com/article/10.1007/s11262-022-01952-2#Sec2 |
genre |
Nyctalus noctula |
genre_facet |
Nyctalus noctula |
op_source |
http://lobid.org/resources/99370676569406441#!, 59(2):323-332 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.1007/s11262-022-01952-2 |
container_title |
Virus Genes |
container_volume |
59 |
container_issue |
2 |
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323 |
op_container_end_page |
332 |
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1779318434059780096 |