Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota)
PREMISE OF THE STUDY: Usnea antarctica and U. aurantiacoatra Parmeliaceae) are common lichens in the maritime Antarctic. These species share the same habitats on King George Island (South Shetland Islands, Antarctica) and are distinguishable based on reproductive strategies. METHODS AND RESULTS: We...
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Online Access: | https://repository.publisso.de/resource/frl:6406799 https://doi.org/10.3732/apps.1700054 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628029/ |
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ftleibnizopen:oai:oai.leibnizopen.de:CNlLoYoBbHMkKcxz_GU8 2023-10-09T21:46:18+02:00 Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) Lagostina,Elisa Dal Grande, Francesco Ott, Sieglinde Printzen, Christian 2017 https://repository.publisso.de/resource/frl:6406799 https://doi.org/10.3732/apps.1700054 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628029/ eng eng http:/creativecommons.org/licenses/by/4.0/ Applications in Plant Sciences, 5(9):1700054 microsatellites Antarctic lichens Usnea antarctica Parmeliaceae Usnea aurantiacoatra 2017 ftleibnizopen https://doi.org/10.3732/apps.1700054 2023-09-17T23:09:37Z PREMISE OF THE STUDY: Usnea antarctica and U. aurantiacoatra Parmeliaceae) are common lichens in the maritime Antarctic. These species share the same habitats on King George Island (South Shetland Islands, Antarctica) and are distinguishable based on reproductive strategies. METHODS AND RESULTS: We developed 23 fungus-specific simple sequence repeat (SSR) markers that cross-amplify between the two species. We used a low-coverage genome-skimming approach on one sample of each species to identify SSR repeats in the two species. Primers were designed for 3–4-bp repeats, and only the loci common to both species were selected for further analyses. Seventy-seven samples of the two species were selected to assess fungal specificity, genetic variability, and linkage of the markers. In addition, we tested cross-amplification in other Usnea species. CONCLUSIONS: The 23 newly designed SSR markers are suitable for population genetic and phylogeographic studies of Usnea species. Other/Unknown Material Antarc* Antarctic Antarctica King George Island South Shetland Islands Usnea antarctica LeibnizOpen (The Leibniz Association) Antarctic The Antarctic King George Island South Shetland Islands Applications in Plant Sciences 5 9 |
institution |
Open Polar |
collection |
LeibnizOpen (The Leibniz Association) |
op_collection_id |
ftleibnizopen |
language |
English |
topic |
microsatellites Antarctic lichens Usnea antarctica Parmeliaceae Usnea aurantiacoatra |
spellingShingle |
microsatellites Antarctic lichens Usnea antarctica Parmeliaceae Usnea aurantiacoatra Lagostina,Elisa Dal Grande, Francesco Ott, Sieglinde Printzen, Christian Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
topic_facet |
microsatellites Antarctic lichens Usnea antarctica Parmeliaceae Usnea aurantiacoatra |
description |
PREMISE OF THE STUDY: Usnea antarctica and U. aurantiacoatra Parmeliaceae) are common lichens in the maritime Antarctic. These species share the same habitats on King George Island (South Shetland Islands, Antarctica) and are distinguishable based on reproductive strategies. METHODS AND RESULTS: We developed 23 fungus-specific simple sequence repeat (SSR) markers that cross-amplify between the two species. We used a low-coverage genome-skimming approach on one sample of each species to identify SSR repeats in the two species. Primers were designed for 3–4-bp repeats, and only the loci common to both species were selected for further analyses. Seventy-seven samples of the two species were selected to assess fungal specificity, genetic variability, and linkage of the markers. In addition, we tested cross-amplification in other Usnea species. CONCLUSIONS: The 23 newly designed SSR markers are suitable for population genetic and phylogeographic studies of Usnea species. |
author |
Lagostina,Elisa Dal Grande, Francesco Ott, Sieglinde Printzen, Christian |
author_facet |
Lagostina,Elisa Dal Grande, Francesco Ott, Sieglinde Printzen, Christian |
author_sort |
Lagostina,Elisa |
title |
Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
title_short |
Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
title_full |
Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
title_fullStr |
Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
title_full_unstemmed |
Fungus-specific SSR markers in the Antarctic lichens Usnea antarctica and U. aurantiacoatra (Parmeliaceae, Ascomycota) |
title_sort |
fungus-specific ssr markers in the antarctic lichens usnea antarctica and u. aurantiacoatra (parmeliaceae, ascomycota) |
publishDate |
2017 |
url |
https://repository.publisso.de/resource/frl:6406799 https://doi.org/10.3732/apps.1700054 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628029/ |
geographic |
Antarctic The Antarctic King George Island South Shetland Islands |
geographic_facet |
Antarctic The Antarctic King George Island South Shetland Islands |
genre |
Antarc* Antarctic Antarctica King George Island South Shetland Islands Usnea antarctica |
genre_facet |
Antarc* Antarctic Antarctica King George Island South Shetland Islands Usnea antarctica |
op_source |
Applications in Plant Sciences, 5(9):1700054 |
op_rights |
http:/creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3732/apps.1700054 |
container_title |
Applications in Plant Sciences |
container_volume |
5 |
container_issue |
9 |
_version_ |
1779321987466788864 |