Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis)
Salmon lice (Lepeophtheirus salmonis) are parasitic copepods, living mainly on Atlantic salmon and leading to large economical losses in aquaculture every year. Due to the emergence of resistances to several drugs, alternative treatments are developed, including treatment with hydrogen peroxide, fre...
Published in: | Cell Stress and Chaperones |
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Online Access: | https://repository.publisso.de/resource/frl:6410419 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741587/ https://doi.org/10.1007/s12192-017-0830-9 |
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ftleibnizopen:oai:oai.leibnizopen.de:0C_NeYsBBwLIz6xGtTqD 2023-11-12T04:14:41+01:00 Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) Borchel, Andreas Komisarczuk, Anna Z. Rebl, Alexander Goldammer, Tom Nilsen, Frank 2017 https://repository.publisso.de/resource/frl:6410419 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741587/ https://doi.org/10.1007/s12192-017-0830-9 eng eng Cell stress & chaperones, 23(1): 127-139 2017 ftleibnizopen https://doi.org/10.1007/s12192-017-0830-9 2023-10-30T00:26:18Z Salmon lice (Lepeophtheirus salmonis) are parasitic copepods, living mainly on Atlantic salmon and leading to large economical losses in aquaculture every year. Due to the emergence of resistances to several drugs, alternative treatments are developed, including treatment with hydrogen peroxide, freshwater or thermal treatment. The present study gives a first overview of the thermotolerance and stress response of salmon lice. Sea lice nauplii acclimated to 10 °C can survive heat shocks up to 30 °C and are capable of hardening by a sublethal heat shock. We searched in the genome for heat shock protein (HSP) encoding genes and tested their inducibility after heat shock, changes in salinity and treatment with hydrogen peroxide, employing microfluidic qPCRs. We assessed 38 candidate genes, belonging to the small HSP, HSP40, HSP70 and HSP90 families. Nine of these genes showed strong induction after a non-lethal heat shock. In contrast, only three and two of these genes were induced after changes in salinity and incubation in hydrogen peroxide, respectively. This work provides the basis for further work on the stress response on the economically important parasite L. salmonis. Other/Unknown Material Atlantic salmon Copepods Unknown Cell Stress and Chaperones 23 1 127 139 |
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English |
description |
Salmon lice (Lepeophtheirus salmonis) are parasitic copepods, living mainly on Atlantic salmon and leading to large economical losses in aquaculture every year. Due to the emergence of resistances to several drugs, alternative treatments are developed, including treatment with hydrogen peroxide, freshwater or thermal treatment. The present study gives a first overview of the thermotolerance and stress response of salmon lice. Sea lice nauplii acclimated to 10 °C can survive heat shocks up to 30 °C and are capable of hardening by a sublethal heat shock. We searched in the genome for heat shock protein (HSP) encoding genes and tested their inducibility after heat shock, changes in salinity and treatment with hydrogen peroxide, employing microfluidic qPCRs. We assessed 38 candidate genes, belonging to the small HSP, HSP40, HSP70 and HSP90 families. Nine of these genes showed strong induction after a non-lethal heat shock. In contrast, only three and two of these genes were induced after changes in salinity and incubation in hydrogen peroxide, respectively. This work provides the basis for further work on the stress response on the economically important parasite L. salmonis. |
author |
Borchel, Andreas Komisarczuk, Anna Z. Rebl, Alexander Goldammer, Tom Nilsen, Frank |
spellingShingle |
Borchel, Andreas Komisarczuk, Anna Z. Rebl, Alexander Goldammer, Tom Nilsen, Frank Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
author_facet |
Borchel, Andreas Komisarczuk, Anna Z. Rebl, Alexander Goldammer, Tom Nilsen, Frank |
author_sort |
Borchel, Andreas |
title |
Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
title_short |
Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
title_full |
Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
title_fullStr |
Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
title_full_unstemmed |
Systematic identification and characterization of stress-inducible heat shock proteins (HSPs) in the salmon louse (Lepeophtheirus salmonis) |
title_sort |
systematic identification and characterization of stress-inducible heat shock proteins (hsps) in the salmon louse (lepeophtheirus salmonis) |
publishDate |
2017 |
url |
https://repository.publisso.de/resource/frl:6410419 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741587/ https://doi.org/10.1007/s12192-017-0830-9 |
genre |
Atlantic salmon Copepods |
genre_facet |
Atlantic salmon Copepods |
op_source |
Cell stress & chaperones, 23(1): 127-139 |
op_doi |
https://doi.org/10.1007/s12192-017-0830-9 |
container_title |
Cell Stress and Chaperones |
container_volume |
23 |
container_issue |
1 |
container_start_page |
127 |
op_container_end_page |
139 |
_version_ |
1782332274013896704 |