Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral)
While amino acid (AA) geochronology has been widely applied to foraminiferal biomineral proteins, there has been limited assessment of the potential of isolating an ‘intra-crystalline’ fraction of proteins to improve the reliability of AA geochronology for foraminifera. In this study, bleaching expe...
Published in: | Quaternary Geochronology |
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Language: | English |
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2021
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Online Access: | https://eprints.whiterose.ac.uk/167737/ https://eprints.whiterose.ac.uk/167737/1/Wheeler_et_al_2020_QG_Nps_AAR_SI_code.R https://eprints.whiterose.ac.uk/167737/2/Wheeler_et_al_2020_QG_Nps_AAR_AAM.docx https://eprints.whiterose.ac.uk/167737/3/Wheeler_et_al_2020_QG_Nps_AAR_AAM_SI.xlsx https://doi.org/10.1016/j.quageo.2020.101131 |
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ftleedsuniv:oai:eprints.whiterose.ac.uk:167737 2024-04-28T08:28:34+00:00 Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) Wheeler, Lucy J. Penkman, Kirsty E.H. Sejrup, Hans Petter 2021-02 other text https://eprints.whiterose.ac.uk/167737/ https://eprints.whiterose.ac.uk/167737/1/Wheeler_et_al_2020_QG_Nps_AAR_SI_code.R https://eprints.whiterose.ac.uk/167737/2/Wheeler_et_al_2020_QG_Nps_AAR_AAM.docx https://eprints.whiterose.ac.uk/167737/3/Wheeler_et_al_2020_QG_Nps_AAR_AAM_SI.xlsx https://doi.org/10.1016/j.quageo.2020.101131 en eng https://eprints.whiterose.ac.uk/167737/1/Wheeler_et_al_2020_QG_Nps_AAR_SI_code.R https://eprints.whiterose.ac.uk/167737/2/Wheeler_et_al_2020_QG_Nps_AAR_AAM.docx https://eprints.whiterose.ac.uk/167737/3/Wheeler_et_al_2020_QG_Nps_AAR_AAM_SI.xlsx Wheeler, Lucy J., Penkman, Kirsty E.H. orcid.org/0000-0002-6226-9799 and Sejrup, Hans Petter (2021) Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral). Quaternary Geochronology. 101131. ISSN 1871-1014 cc_by_nc_nd Article PeerReviewed 2021 ftleedsuniv https://doi.org/10.1016/j.quageo.2020.101131 2024-04-09T23:38:30Z While amino acid (AA) geochronology has been widely applied to foraminiferal biomineral proteins, there has been limited assessment of the potential of isolating an ‘intra-crystalline’ fraction of proteins to improve the reliability of AA geochronology for foraminifera. In this study, bleaching experiments were carried out on the foraminifer Neogloboquadrina pachyderma (sinistral) from an independently dated core from the south eastern Norwegian Sea spanning the last 120 ka. Results show that this species contains a bleach-resistant fraction of biomineral proteins, and that this intra-crystalline fraction may behave as a closed system. Racemisation in both the intra-crystalline and whole-protein fractions was found to systematically increase with time. Isoleucine epimerisation showed reliable trends with depth when analysed by ion exchange chromatography (IEC); analysis by reverse-phase liquid chromatography (RP-HPLC) resulted in reliable age-D/L relationships for aspartic acid, glutamic acid and alanine. Isolating the intra-crystalline fraction with a 48 h bleach treatment reduced the influence of contamination in younger material. Therefore, this paper recommends that the intra-crystalline approach is used when analysing Neogloboquadrina pachyderma (sinistral) for AA geochronology, especially for younger samples. Material-specific regression equations developed in this study successfully convert IEC A/I to RP-HPLC D/L values for aspartic acid, glutamic acid and alanine, enabling integration of geochronologies developed using different methods for this class of biomineral. Article in Journal/Newspaper Neogloboquadrina pachyderma Norwegian Sea White Rose Research Online (Universities of Leeds, Sheffield & York) Quaternary Geochronology 61 101131 |
institution |
Open Polar |
collection |
White Rose Research Online (Universities of Leeds, Sheffield & York) |
op_collection_id |
ftleedsuniv |
language |
English |
description |
While amino acid (AA) geochronology has been widely applied to foraminiferal biomineral proteins, there has been limited assessment of the potential of isolating an ‘intra-crystalline’ fraction of proteins to improve the reliability of AA geochronology for foraminifera. In this study, bleaching experiments were carried out on the foraminifer Neogloboquadrina pachyderma (sinistral) from an independently dated core from the south eastern Norwegian Sea spanning the last 120 ka. Results show that this species contains a bleach-resistant fraction of biomineral proteins, and that this intra-crystalline fraction may behave as a closed system. Racemisation in both the intra-crystalline and whole-protein fractions was found to systematically increase with time. Isoleucine epimerisation showed reliable trends with depth when analysed by ion exchange chromatography (IEC); analysis by reverse-phase liquid chromatography (RP-HPLC) resulted in reliable age-D/L relationships for aspartic acid, glutamic acid and alanine. Isolating the intra-crystalline fraction with a 48 h bleach treatment reduced the influence of contamination in younger material. Therefore, this paper recommends that the intra-crystalline approach is used when analysing Neogloboquadrina pachyderma (sinistral) for AA geochronology, especially for younger samples. Material-specific regression equations developed in this study successfully convert IEC A/I to RP-HPLC D/L values for aspartic acid, glutamic acid and alanine, enabling integration of geochronologies developed using different methods for this class of biomineral. |
format |
Article in Journal/Newspaper |
author |
Wheeler, Lucy J. Penkman, Kirsty E.H. Sejrup, Hans Petter |
spellingShingle |
Wheeler, Lucy J. Penkman, Kirsty E.H. Sejrup, Hans Petter Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
author_facet |
Wheeler, Lucy J. Penkman, Kirsty E.H. Sejrup, Hans Petter |
author_sort |
Wheeler, Lucy J. |
title |
Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
title_short |
Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
title_full |
Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
title_fullStr |
Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
title_full_unstemmed |
Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral) |
title_sort |
assessing the intra-crystalline approach to amino acid geochronology of neogloboquadrina pachyderma (sinistral) |
publishDate |
2021 |
url |
https://eprints.whiterose.ac.uk/167737/ https://eprints.whiterose.ac.uk/167737/1/Wheeler_et_al_2020_QG_Nps_AAR_SI_code.R https://eprints.whiterose.ac.uk/167737/2/Wheeler_et_al_2020_QG_Nps_AAR_AAM.docx https://eprints.whiterose.ac.uk/167737/3/Wheeler_et_al_2020_QG_Nps_AAR_AAM_SI.xlsx https://doi.org/10.1016/j.quageo.2020.101131 |
genre |
Neogloboquadrina pachyderma Norwegian Sea |
genre_facet |
Neogloboquadrina pachyderma Norwegian Sea |
op_relation |
https://eprints.whiterose.ac.uk/167737/1/Wheeler_et_al_2020_QG_Nps_AAR_SI_code.R https://eprints.whiterose.ac.uk/167737/2/Wheeler_et_al_2020_QG_Nps_AAR_AAM.docx https://eprints.whiterose.ac.uk/167737/3/Wheeler_et_al_2020_QG_Nps_AAR_AAM_SI.xlsx Wheeler, Lucy J., Penkman, Kirsty E.H. orcid.org/0000-0002-6226-9799 and Sejrup, Hans Petter (2021) Assessing the intra-crystalline approach to amino acid geochronology of Neogloboquadrina pachyderma (sinistral). Quaternary Geochronology. 101131. ISSN 1871-1014 |
op_rights |
cc_by_nc_nd |
op_doi |
https://doi.org/10.1016/j.quageo.2020.101131 |
container_title |
Quaternary Geochronology |
container_volume |
61 |
container_start_page |
101131 |
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1797587056533176320 |