Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease

Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-ass...

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Main Authors: Marsian, J, Hurdiss, DL, Ranson, NA, Ritala, A, Paley, R, Cano, I, Lomonossoff, GP
Other Authors: Guzzo, F
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media 2019
Subjects:
atlantic cod
Online Access:https://eprints.whiterose.ac.uk/149337/
https://eprints.whiterose.ac.uk/149337/1/fpls-10-00880.pdf
id ftleedsuniv:oai:eprints.whiterose.ac.uk:149337
record_format openpolar
spelling ftleedsuniv:oai:eprints.whiterose.ac.uk:149337 2023-05-15T15:27:19+02:00 Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease Marsian, J Hurdiss, DL Ranson, NA Ritala, A Paley, R Cano, I Lomonossoff, GP Guzzo, F 2019-07 text https://eprints.whiterose.ac.uk/149337/ https://eprints.whiterose.ac.uk/149337/1/fpls-10-00880.pdf en eng Frontiers Media https://eprints.whiterose.ac.uk/149337/1/fpls-10-00880.pdf Marsian, J, Hurdiss, DL, Ranson, NA orcid.org/0000-0002-3640-5275 et al. (4 more authors) (2019) Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease. Frontiers in Plant Science, 10. 880. ISSN 1664-462X cc_by_4 CC-BY Article NonPeerReviewed 2019 ftleedsuniv 2023-01-30T22:21:17Z Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. Article in Journal/Newspaper atlantic cod White Rose Research Online (Universities of Leeds, Sheffield & York)
institution Open Polar
collection White Rose Research Online (Universities of Leeds, Sheffield & York)
op_collection_id ftleedsuniv
language English
description Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
author2 Guzzo, F
format Article in Journal/Newspaper
author Marsian, J
Hurdiss, DL
Ranson, NA
Ritala, A
Paley, R
Cano, I
Lomonossoff, GP
spellingShingle Marsian, J
Hurdiss, DL
Ranson, NA
Ritala, A
Paley, R
Cano, I
Lomonossoff, GP
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
author_facet Marsian, J
Hurdiss, DL
Ranson, NA
Ritala, A
Paley, R
Cano, I
Lomonossoff, GP
author_sort Marsian, J
title Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_short Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_fullStr Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_full_unstemmed Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease
title_sort plant-made nervous necrosis virus-like particles protect fish against disease
publisher Frontiers Media
publishDate 2019
url https://eprints.whiterose.ac.uk/149337/
https://eprints.whiterose.ac.uk/149337/1/fpls-10-00880.pdf
genre atlantic cod
genre_facet atlantic cod
op_relation https://eprints.whiterose.ac.uk/149337/1/fpls-10-00880.pdf
Marsian, J, Hurdiss, DL, Ranson, NA orcid.org/0000-0002-3640-5275 et al. (4 more authors) (2019) Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease. Frontiers in Plant Science, 10. 880. ISSN 1664-462X
op_rights cc_by_4
op_rightsnorm CC-BY
_version_ 1766357768219918336

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