Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff
By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruf...
Published in: | Ecology and Evolution |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley Open Access
2012
|
Subjects: | |
Online Access: | https://eprints.whiterose.ac.uk/108352/ https://eprints.whiterose.ac.uk/108352/1/Gene%20expression%20divergence%20and%20nucleotide%20differentiation%20between%20males%20of%20different%20color%20morphs%20and%20mating%20strategies%20in%20the%20ruff.pdf https://doi.org/10.1002/ece3.370 |
id |
ftleedsuniv:oai:eprints.whiterose.ac.uk:108352 |
---|---|
record_format |
openpolar |
spelling |
ftleedsuniv:oai:eprints.whiterose.ac.uk:108352 2023-05-15T17:58:41+02:00 Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff Ekblom, R. Farrell, L. L. Lank, D. B. Burke, T. 2012-10-11 text https://eprints.whiterose.ac.uk/108352/ https://eprints.whiterose.ac.uk/108352/1/Gene%20expression%20divergence%20and%20nucleotide%20differentiation%20between%20males%20of%20different%20color%20morphs%20and%20mating%20strategies%20in%20the%20ruff.pdf https://doi.org/10.1002/ece3.370 en eng Wiley Open Access https://eprints.whiterose.ac.uk/108352/1/Gene%20expression%20divergence%20and%20nucleotide%20differentiation%20between%20males%20of%20different%20color%20morphs%20and%20mating%20strategies%20in%20the%20ruff.pdf Ekblom, R., Farrell, L. L., Lank, D. B. et al. (1 more author) (2012) Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff. Ecology and Evolution, 2 (10). pp. 2485-2500. ISSN 2045-7758 cc_by_nc_3 CC-BY-NC Article PeerReviewed 2012 ftleedsuniv https://doi.org/10.1002/ece3.370 2023-01-30T21:48:57Z By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruff (Philomachus pugnax). Ruff males show enormous variation in the coloration of ornamental feathers, used for individual recognition. This polymorphism is linked to reproductive strategies, with dark males (Independents) defending territories on leks against other Independents, whereas white morphs (Satellites) co-occupy Independent's courts without agonistic interactions. Previous work found a strong genetic component for mating strategy, but the genes involved were not identified. We present feather transcriptome data of more than 6,000 de-novo sequenced ruff genes (although with limited coverage for many of them). None of the identified genes showed significant expression divergence between males, but many genetic markers showed nucleotide differentiation between different color morphs and mating strategies. These include several feather keratin genes, splicing factors, and the Xg blood-group gene. Many of the genes with significant genetic structure between mating strategies have not yet been annotated and their functions remain to be elucidated. We also conducted in-depth investigations of 28 pre-identified coloration candidate genes. Two of these (EDNRB and TYR) were specifically expressed in black- and rust-colored males, respectively. We have demonstrated the utility of next generation transcriptome sequencing for identifying and genotyping large number of genetic markers in a non-model species without previous genomic resources, and highlight the potential of this approach for addressing the genetic basis of ecologically important variation. Article in Journal/Newspaper Philomachus pugnax Ruff White Rose Research Online (Universities of Leeds, Sheffield & York) Ecology and Evolution 2 10 2485 2505 |
institution |
Open Polar |
collection |
White Rose Research Online (Universities of Leeds, Sheffield & York) |
op_collection_id |
ftleedsuniv |
language |
English |
description |
By next generation transcriptome sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have used this approach (RNA-Seq) to investigate the genetic basis for differences in plumage coloration and mating strategies in a non-model bird species, the ruff (Philomachus pugnax). Ruff males show enormous variation in the coloration of ornamental feathers, used for individual recognition. This polymorphism is linked to reproductive strategies, with dark males (Independents) defending territories on leks against other Independents, whereas white morphs (Satellites) co-occupy Independent's courts without agonistic interactions. Previous work found a strong genetic component for mating strategy, but the genes involved were not identified. We present feather transcriptome data of more than 6,000 de-novo sequenced ruff genes (although with limited coverage for many of them). None of the identified genes showed significant expression divergence between males, but many genetic markers showed nucleotide differentiation between different color morphs and mating strategies. These include several feather keratin genes, splicing factors, and the Xg blood-group gene. Many of the genes with significant genetic structure between mating strategies have not yet been annotated and their functions remain to be elucidated. We also conducted in-depth investigations of 28 pre-identified coloration candidate genes. Two of these (EDNRB and TYR) were specifically expressed in black- and rust-colored males, respectively. We have demonstrated the utility of next generation transcriptome sequencing for identifying and genotyping large number of genetic markers in a non-model species without previous genomic resources, and highlight the potential of this approach for addressing the genetic basis of ecologically important variation. |
format |
Article in Journal/Newspaper |
author |
Ekblom, R. Farrell, L. L. Lank, D. B. Burke, T. |
spellingShingle |
Ekblom, R. Farrell, L. L. Lank, D. B. Burke, T. Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
author_facet |
Ekblom, R. Farrell, L. L. Lank, D. B. Burke, T. |
author_sort |
Ekblom, R. |
title |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_short |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_full |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_fullStr |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_full_unstemmed |
Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
title_sort |
gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff |
publisher |
Wiley Open Access |
publishDate |
2012 |
url |
https://eprints.whiterose.ac.uk/108352/ https://eprints.whiterose.ac.uk/108352/1/Gene%20expression%20divergence%20and%20nucleotide%20differentiation%20between%20males%20of%20different%20color%20morphs%20and%20mating%20strategies%20in%20the%20ruff.pdf https://doi.org/10.1002/ece3.370 |
genre |
Philomachus pugnax Ruff |
genre_facet |
Philomachus pugnax Ruff |
op_relation |
https://eprints.whiterose.ac.uk/108352/1/Gene%20expression%20divergence%20and%20nucleotide%20differentiation%20between%20males%20of%20different%20color%20morphs%20and%20mating%20strategies%20in%20the%20ruff.pdf Ekblom, R., Farrell, L. L., Lank, D. B. et al. (1 more author) (2012) Gene expression divergence and nucleotide differentiation between males of different color morphs and mating strategies in the ruff. Ecology and Evolution, 2 (10). pp. 2485-2500. ISSN 2045-7758 |
op_rights |
cc_by_nc_3 |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1002/ece3.370 |
container_title |
Ecology and Evolution |
container_volume |
2 |
container_issue |
10 |
container_start_page |
2485 |
op_container_end_page |
2505 |
_version_ |
1766167381037547520 |