Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease
Copyright © 2020 American Chemical Society. RNA aptamers have garnered attention for diagnostic applications due to their ability to recognize diverse targets. Oligomers of 42-mer amyloid β-protein (Aβ42), whose accumulation is relevant to the pathology of Alzheimer's disease (AD), are among th...
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ftlatrobeunivfig:oai:figshare.com:article/13601138 2023-05-15T14:58:39+02:00 Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease Y Obata K Murakami T Kawase K Hirose Naotaka Izuo T Shimizu K Irie 2021-01-17T23:44:57Z https://doi.org/10.26181/6004cbfa649cc https://figshare.com/articles/journal_contribution/Detection_of_Amyloid_Oligomers_with_RNA_Aptamers_in_AppNL-G-F_NL-G-F_Mice_A_Model_of_Arctic_Alzheimer_s_Disease/13601138 unknown doi:10.26181/6004cbfa649cc https://figshare.com/articles/journal_contribution/Detection_of_Amyloid_Oligomers_with_RNA_Aptamers_in_AppNL-G-F_NL-G-F_Mice_A_Model_of_Arctic_Alzheimer_s_Disease/13601138 CC BY-NC 4.0 CC-BY-NC Uncategorized Science & Technology Physical Sciences Chemistry Multidisciplinary PRION PROTEIN SELECTION DNA CONFORMATIONS Text Journal contribution 2021 ftlatrobeunivfig https://doi.org/10.26181/6004cbfa649cc 2021-11-18T00:07:07Z Copyright © 2020 American Chemical Society. RNA aptamers have garnered attention for diagnostic applications due to their ability to recognize diverse targets. Oligomers of 42-mer amyloid β-protein (Aβ42), whose accumulation is relevant to the pathology of Alzheimer's disease (AD), are among the most difficult molecules for aptamer recognition because they are prone to aggregate in heterogeneous forms. In addition to designing haptens for in vitro selection of aptamers, the difficulties involved in determining their effect on Aβ42 oligomerization impede aptamer research. We previously developed three RNA aptamers (E22P-AbD4, -AbD31, and -AbD43) with high affinity for protofibrils (PFs) derived from a toxic Aβ42 dimer. Notably, these aptamers recognized diffuse staining, which likely originated from PFs or higher-order oligomers with curvilinear structures in a knock-in AppNL-G-F/NL-G-F mouse, carrying the Arctic mutation that preferentially induced the formation of PFs, in addition to a PS2Tg2576 mouse. To determine which oligomeric sizes were mainly altered by the aptamer, ion mobility-mass spectrometry (IM-MS) was carried out. One aptamer, E22P-AbD43, formed adducts with the Aβ42 monomer and dimer, leading to suppression of further oligomerization. These findings support the utility of these aptamers as diagnostics for AD. Other Non-Article Part of Journal/Newspaper Arctic La Trobe University (Melbourne): Figshare Arctic |
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La Trobe University (Melbourne): Figshare |
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Uncategorized Science & Technology Physical Sciences Chemistry Multidisciplinary PRION PROTEIN SELECTION DNA CONFORMATIONS |
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Uncategorized Science & Technology Physical Sciences Chemistry Multidisciplinary PRION PROTEIN SELECTION DNA CONFORMATIONS Y Obata K Murakami T Kawase K Hirose Naotaka Izuo T Shimizu K Irie Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
topic_facet |
Uncategorized Science & Technology Physical Sciences Chemistry Multidisciplinary PRION PROTEIN SELECTION DNA CONFORMATIONS |
description |
Copyright © 2020 American Chemical Society. RNA aptamers have garnered attention for diagnostic applications due to their ability to recognize diverse targets. Oligomers of 42-mer amyloid β-protein (Aβ42), whose accumulation is relevant to the pathology of Alzheimer's disease (AD), are among the most difficult molecules for aptamer recognition because they are prone to aggregate in heterogeneous forms. In addition to designing haptens for in vitro selection of aptamers, the difficulties involved in determining their effect on Aβ42 oligomerization impede aptamer research. We previously developed three RNA aptamers (E22P-AbD4, -AbD31, and -AbD43) with high affinity for protofibrils (PFs) derived from a toxic Aβ42 dimer. Notably, these aptamers recognized diffuse staining, which likely originated from PFs or higher-order oligomers with curvilinear structures in a knock-in AppNL-G-F/NL-G-F mouse, carrying the Arctic mutation that preferentially induced the formation of PFs, in addition to a PS2Tg2576 mouse. To determine which oligomeric sizes were mainly altered by the aptamer, ion mobility-mass spectrometry (IM-MS) was carried out. One aptamer, E22P-AbD43, formed adducts with the Aβ42 monomer and dimer, leading to suppression of further oligomerization. These findings support the utility of these aptamers as diagnostics for AD. |
format |
Other Non-Article Part of Journal/Newspaper |
author |
Y Obata K Murakami T Kawase K Hirose Naotaka Izuo T Shimizu K Irie |
author_facet |
Y Obata K Murakami T Kawase K Hirose Naotaka Izuo T Shimizu K Irie |
author_sort |
Y Obata |
title |
Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
title_short |
Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
title_full |
Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
title_fullStr |
Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
title_full_unstemmed |
Detection of Amyloid β Oligomers with RNA Aptamers in AppNL-G-F/NL-G-F Mice: A Model of Arctic Alzheimer’s Disease |
title_sort |
detection of amyloid β oligomers with rna aptamers in appnl-g-f/nl-g-f mice: a model of arctic alzheimer’s disease |
publishDate |
2021 |
url |
https://doi.org/10.26181/6004cbfa649cc https://figshare.com/articles/journal_contribution/Detection_of_Amyloid_Oligomers_with_RNA_Aptamers_in_AppNL-G-F_NL-G-F_Mice_A_Model_of_Arctic_Alzheimer_s_Disease/13601138 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
doi:10.26181/6004cbfa649cc https://figshare.com/articles/journal_contribution/Detection_of_Amyloid_Oligomers_with_RNA_Aptamers_in_AppNL-G-F_NL-G-F_Mice_A_Model_of_Arctic_Alzheimer_s_Disease/13601138 |
op_rights |
CC BY-NC 4.0 |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.26181/6004cbfa649cc |
_version_ |
1766330775358144512 |