Detection of Local Prostate Metabolites by HRMAs NMR Spectroscopy: A Comparative Study of Human and Rat Prostate Tissues

• Main Author: Stenman
• Other Authors: Katarina Stenman, Izabella Surowiec, Henrik Antti, Katrine Riklund, Pär Stattin, Anders Bergh and Gerhard Gröbner
• Language: English
• Published: Libertas Academica 2010
• Subjects: Magnetic Resonance Insights; Volume: 2010; Issue: 4; Northern Sweden
• Online Access: http://www.la-press.com/redirect_file.php?fileId=3216&filename=2356-MRI-Detection-of-Local-Prostate-Metabolites-by-HRMAs-NMR-Spectroscopy:-A-C.pdf&fileType=pdf

Katarina Stenman1, Izabella Surowiec4, Henrik Antti4, Katrine Riklund1, Pär Stattin3, Anders Bergh2 and Gerhard Gröbner41Departments of Radiation Sciences, Diagnostic Radiology, 2Medical Biosciences, Pathology, 3Surgical and Perioperative Sciences, Urology and Andrology, Umeå University and University Hospital of Northern Sweden, Umeå, Sweden. 4Department of Chemistry, Umeå University, Umeå, Sweden. AbstractThe use of magnetic resonance spectroscopy (MRS) for the detection of in-vivo metabolic perturbations is increasing in popularity in Prostate Cancer (PCa) research on both humans and rodent models. However, there are distinct metabolic differences between species and prostate areas; a fact making general conclusions about PCa difficult. Here, we use High Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HRMAS NMR) spectroscopy to provide tissue specific identification of metabolites and their relative ratios; information useful in providing insight into the biochemical pathways of the prostate. As our NMR-based approach reveals, human and rat prostate tissues have different metabolic signatures as reflected in numerous key metabolites, including citrate and choline compounds, but also aspartate, lysine, taurine, glutamate, glutamine, creatine and inositol. In general, distribution of these metabolites is not only highly dependent on the species (human versus rat), but also on the location (lobe/zone) in the prostate tissue and the sample pathology; an observation making HRMAS NMR of intact tissue samples a promising method for extracting differences and common features in various experimental prostate cancer models.