Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions.
To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download Understanding the structure of a bacterial population is essential in order to understand bacterial evolution. Estima...
Published in: | Frontiers in Microbiology |
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Main Authors: | , , , , , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Frontiers Research Foundation
2019
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Online Access: | http://hdl.handle.net/2336/620887 https://doi.org/10.3389/fmicb.2019.00317 |
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ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/620887 |
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openpolar |
institution |
Open Polar |
collection |
Hirsla - Landspítali University Hospital research archive |
op_collection_id |
ftlandspitaliuni |
language |
English |
topic |
accessory genome bacterial population structure core genome next generation sequencing pan-genome pneumococcus Gerlar Landfræðileg útbreiðsla Bakteríusjúkdómar Streptococcus pneumoniae Bacterial Structures Geographic Locations |
spellingShingle |
accessory genome bacterial population structure core genome next generation sequencing pan-genome pneumococcus Gerlar Landfræðileg útbreiðsla Bakteríusjúkdómar Streptococcus pneumoniae Bacterial Structures Geographic Locations van Tonder, Andries J Bray, James E Jolley, Keith A Jansen van Rensburg, Melissa Quirk, Sigríður J Haraldsson, Gunnsteinn Maiden, Martin C J Bentley, Stephen D Haraldsson, Ásgeir Erlendsdóttir, Helga Kristinsson, Karl G Brueggemann, Angela B Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
topic_facet |
accessory genome bacterial population structure core genome next generation sequencing pan-genome pneumococcus Gerlar Landfræðileg útbreiðsla Bakteríusjúkdómar Streptococcus pneumoniae Bacterial Structures Geographic Locations |
description |
To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download Understanding the structure of a bacterial population is essential in order to understand bacterial evolution. Estimating the core genome (those genes common to all, or nearly all, strains of a species) is a key component of such analyses. The size and composition of the core genome varies by dataset, but we hypothesized that the variation between different collections of the same bacterial species would be minimal. To investigate this, we analyzed the genome sequences of 3,118 pneumococci recovered from healthy individuals in Reykjavik (Iceland), Southampton (United Kingdom), Boston (United States), and Maela (Thailand). The analyses revealed a "supercore" genome (genes shared by all 3,118 pneumococci) of 558 genes, although an additional 354 core genes were shared by pneumococci from Reykjavik, Southampton, and Boston. Overall, the size and composition of the core and pan-genomes among pneumococci recovered in Reykjavik, Southampton, and Boston were similar. Maela pneumococci were distinctly different in that they had a smaller core genome and larger pan-genome. The pan-genome of Maela pneumococci contained several >25 Kb sequence regions (flanked by pneumococcal genes) that were homologous to genomic regions found in other bacterial species. Overall, our work revealed that some subsets of the global pneumococcal population are highly heterogeneous, and our hypothesis was rejected. This is an important finding in terms of understanding genetic variation among pneumococci and is also an essential point of consideration before generalizing the findings from a single dataset to the wider pneumococcal population. Wellcome Trust Biomedical Research Fund award Wellcome Trust Research Fellowship University of Oxford John Fell Fund award Wellcome Trust Eimskipa University Fund GlaxoSmithKline Biologicals SA Landspitali University ... |
author2 |
1 Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom. 2 Parasites and Microbes, Wellcome Sanger Institute, Hinxton, United Kingdom. 3 Department of Zoology, University of Oxford, Oxford, United Kingdom. 4 Department of Medicine, Imperial College London, London, United Kingdom. 5 Clinical Microbiology, University of Iceland and Landspitali University Hospital, Reykjavik, Iceland. 6 Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom. 7 Department of Pathology, University of Cambridge, Cambridge, United Kingdom. 8 Children's Hospital Iceland, Landspitali University Hospital, Reykjavik, Iceland. |
format |
Article in Journal/Newspaper |
author |
van Tonder, Andries J Bray, James E Jolley, Keith A Jansen van Rensburg, Melissa Quirk, Sigríður J Haraldsson, Gunnsteinn Maiden, Martin C J Bentley, Stephen D Haraldsson, Ásgeir Erlendsdóttir, Helga Kristinsson, Karl G Brueggemann, Angela B |
author_facet |
van Tonder, Andries J Bray, James E Jolley, Keith A Jansen van Rensburg, Melissa Quirk, Sigríður J Haraldsson, Gunnsteinn Maiden, Martin C J Bentley, Stephen D Haraldsson, Ásgeir Erlendsdóttir, Helga Kristinsson, Karl G Brueggemann, Angela B |
author_sort |
van Tonder, Andries J |
title |
Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
title_short |
Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
title_full |
Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
title_fullStr |
Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
title_full_unstemmed |
Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. |
title_sort |
genomic analyses of >3,100 nasopharyngeal pneumococci revealed significant differences between pneumococci recovered in four different geographical regions. |
publisher |
Frontiers Research Foundation |
publishDate |
2019 |
url |
http://hdl.handle.net/2336/620887 https://doi.org/10.3389/fmicb.2019.00317 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Frontiers in microbiology |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2019.00317/full Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. 2019, 10: 317 Front Microbiol 1664-302X 30858837 doi:10.3389/fmicb.2019.00317 http://hdl.handle.net/2336/620887 Frontiers in microbiology |
op_rights |
Open Access - Opinn aðgangur |
op_doi |
https://doi.org/10.3389/fmicb.2019.00317 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1766042517524971520 |
spelling |
ftlandspitaliuni:oai:www.hirsla.lsh.is:2336/620887 2023-05-15T16:52:20+02:00 Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. van Tonder, Andries J Bray, James E Jolley, Keith A Jansen van Rensburg, Melissa Quirk, Sigríður J Haraldsson, Gunnsteinn Maiden, Martin C J Bentley, Stephen D Haraldsson, Ásgeir Erlendsdóttir, Helga Kristinsson, Karl G Brueggemann, Angela B 1 Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom. 2 Parasites and Microbes, Wellcome Sanger Institute, Hinxton, United Kingdom. 3 Department of Zoology, University of Oxford, Oxford, United Kingdom. 4 Department of Medicine, Imperial College London, London, United Kingdom. 5 Clinical Microbiology, University of Iceland and Landspitali University Hospital, Reykjavik, Iceland. 6 Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom. 7 Department of Pathology, University of Cambridge, Cambridge, United Kingdom. 8 Children's Hospital Iceland, Landspitali University Hospital, Reykjavik, Iceland. 2019-04 http://hdl.handle.net/2336/620887 https://doi.org/10.3389/fmicb.2019.00317 en eng Frontiers Research Foundation https://www.frontiersin.org/articles/10.3389/fmicb.2019.00317/full Genomic Analyses of >3,100 Nasopharyngeal Pneumococci Revealed Significant Differences Between Pneumococci Recovered in Four Different Geographical Regions. 2019, 10: 317 Front Microbiol 1664-302X 30858837 doi:10.3389/fmicb.2019.00317 http://hdl.handle.net/2336/620887 Frontiers in microbiology Open Access - Opinn aðgangur Frontiers in microbiology accessory genome bacterial population structure core genome next generation sequencing pan-genome pneumococcus Gerlar Landfræðileg útbreiðsla Bakteríusjúkdómar Streptococcus pneumoniae Bacterial Structures Geographic Locations Article 2019 ftlandspitaliuni https://doi.org/10.3389/fmicb.2019.00317 2022-05-29T08:22:25Z To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked Download Understanding the structure of a bacterial population is essential in order to understand bacterial evolution. Estimating the core genome (those genes common to all, or nearly all, strains of a species) is a key component of such analyses. The size and composition of the core genome varies by dataset, but we hypothesized that the variation between different collections of the same bacterial species would be minimal. To investigate this, we analyzed the genome sequences of 3,118 pneumococci recovered from healthy individuals in Reykjavik (Iceland), Southampton (United Kingdom), Boston (United States), and Maela (Thailand). The analyses revealed a "supercore" genome (genes shared by all 3,118 pneumococci) of 558 genes, although an additional 354 core genes were shared by pneumococci from Reykjavik, Southampton, and Boston. Overall, the size and composition of the core and pan-genomes among pneumococci recovered in Reykjavik, Southampton, and Boston were similar. Maela pneumococci were distinctly different in that they had a smaller core genome and larger pan-genome. The pan-genome of Maela pneumococci contained several >25 Kb sequence regions (flanked by pneumococcal genes) that were homologous to genomic regions found in other bacterial species. Overall, our work revealed that some subsets of the global pneumococcal population are highly heterogeneous, and our hypothesis was rejected. This is an important finding in terms of understanding genetic variation among pneumococci and is also an essential point of consideration before generalizing the findings from a single dataset to the wider pneumococcal population. Wellcome Trust Biomedical Research Fund award Wellcome Trust Research Fellowship University of Oxford John Fell Fund award Wellcome Trust Eimskipa University Fund GlaxoSmithKline Biologicals SA Landspitali University ... Article in Journal/Newspaper Iceland Hirsla - Landspítali University Hospital research archive Frontiers in Microbiology 10 |